Lactoperoxidase Antibody

Images

 
Western Blot: Lactoperoxidase Antibody [NB110-61572] - Detection of LPO in MDA-MB231 cell lysate using NB110-61572.

Product Details

Summary
Reactivity Hu, Bv, PmSpecies Glossary
Applications WB
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.7 mg/ml

Lactoperoxidase Antibody Summary

Immunogen
Synthetic peptide made to an internal portion of the human Lactoperoxidase protein (within residues 300-400). [Swiss-Prot# P22079]
Localization
Secreted, extracellular space.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LPO
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
Application Notes
This Lactoperoxidase antibody is useful for Western blot analysis where a band is seen at ~100 kDa. Caution, do not use milk for the blocking or dilution buffers.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Positive Control
HeLa Lysate (NB800-PC1)

Reactivity Notes

Human, monkey and bovine. Immunogen has 81% homology to sheep and 73% homology to mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
1.7 mg/ml
Purity
Immunogen affinity purified

Alternate Names for Lactoperoxidase Antibody

  • EC 1.11.1
  • lactoperoxidase
  • MGC129990
  • MGC129991
  • Salivary peroxidase
  • SAPX
  • SPOEC 1.11.1.7

Background

Lactoperoxidase (LPO) is a heme-containing glycoprotein enzyme of about 78 kD molecular size expressed highly in mammary gland, salivary gland and bronchial submucosal glands, and consequently found in large amounts in secretory fluids such as milk, tears, saliva, and lung fluids. Lactoperoxidase belongs to mammalian peroxidase family with a prosthetic heme group covalently linked to the proteins and other members of this family include myeloperoxidase (MPO), eosinophil peroxidase (EPO), and thyroid peroxidase (TPO). In the presence of H2O2, lactoperoxidase oxidizes thiocyanate (SCN-) more efficiently than other peroxidases, whereas MPO, EPO, and TPO most efficiently oxidize chloride, bromide, and iodide, respectively, to form hypo(pseudo)halides. After extracellular transport, SCN- gets oxidized either by peroxidases to form hypothiocyanite (OSCN-) or nonenzymatically by hypochlorite (OCl-). SCN exporters are the cystic fibrosis transmembrane conductance regulators, calcium-activated chloride channels, and pendrin/Slc26A4. OSCN- is tissue-innocuous but has a broad-spectrum microbicidal and microbistatic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP2-24916
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC, IHC-P
NBP2-24915
Species: Hu, Mu, Rt, Bv
Applications: WB, IHC-P
NBP1-32108
Species: Hu, Rt
Applications: WB, ICC/IF
NBP2-45897
Species: Hu
Applications: WB, IHC
AF3667
Species: Hu, Mu
Applications: WB, Simple Western, IHC, ICC
NBP2-16684
Species: Hu, Mu, Rt
Applications: WB, IHC, IHC-P
NBP1-89726
Species: Hu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-49118
Species: Hu
Applications: IHC, IHC-P
NB300-731
Species: Hu, Mu, Rt, GP, Rb, Sh, Ye
Applications: WB, ChIP, B/N, Flow, GS, ICC/IF, IHC, IHC-P, IP
NBP1-50811
Species: Hu
Applications: WB, ELISA, IA, S-ELISA
NBP1-05027
Species: Hu
Applications: WB, ELISA, IHC, IHC-P, PEP-ELISA, S-ELISA
NBP2-22449
Species: Hu, Mu, Rt, Fe, GP, Rb
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP, GS
NBP2-20383
Species: Hu, Mu
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-34294
Species: Hu, Mu, Rt
Applications: WB, Flow, IHC-P
NB600-41532
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-P
NB600-935
Species: Bv
Applications: WB, ELISA, IP
NB110-61572
Species: Hu, Bv, Pm
Applications: WB

Publications for Lactoperoxidase Antibody (NB110-61572) (0)

There are no publications for Lactoperoxidase Antibody (NB110-61572).
By submitting your publication information earn gift cards and discounts for future purchases.

Reviews for Lactoperoxidase Antibody (NB110-61572) (0)

There are no reviews for Lactoperoxidase Antibody (NB110-61572). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

View specific protocols for Lactoperoxidase Antibody (NB110-61572): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for Lactoperoxidase Antibody (NB110-61572) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-61572AF405
Alexa Fluor 488 Labeled NB110-61572AF488
Alexa Fluor 647 Labeled NB110-61572AF647
Alexa Fluor 700 Labeled NB110-61572AF700
Biotin Labeled NB110-61572B
DyLight 405 Labeled NB110-61572V
DyLight 488 Labeled NB110-61572G
DyLight 550 Labeled NB110-61572R
DyLight 650 Labeled NB110-61572C
HRP Labeled NB110-61572H

Additional Lactoperoxidase Products

Bioinformatics Tool for Lactoperoxidase Antibody (NB110-61572)

Discover related pathways, diseases and genes to Lactoperoxidase Antibody (NB110-61572). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for Lactoperoxidase Antibody (NB110-61572)

Discover more about diseases related to Lactoperoxidase Antibody (NB110-61572).
 

Pathways for Lactoperoxidase Antibody (NB110-61572)

View related products by pathway.

PTMs for Lactoperoxidase Antibody (NB110-61572)

Learn more about PTMs related to Lactoperoxidase Antibody (NB110-61572).
 

Research Areas for Lactoperoxidase Antibody (NB110-61572)

Find related products by research area.

Blogs on Lactoperoxidase

There are no specific blogs for Lactoperoxidase, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our Lactoperoxidase Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol LPO