Lactoperoxidase Antibody [FITC]

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Product Details

Summary
Product Discontinued
View other related Lactoperoxidase Primary Antibodies

Order Details


    • Catalog Number
      NB110-61572F
    • Availability
      Product Discontinued

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Lactoperoxidase Antibody [FITC] Summary

Immunogen
Synthetic peptide made to an internal portion of the human Lactoperoxidase protein (within residues 300-400). [Swiss-Prot# P22079]
Localization
Secreted, extracellular space.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
LPO
Purity
Immunogen affinity purified
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Applications/Dilutions

Dilutions
  • Immunohistochemistry 1:10 - 1:500
Application Notes
This Lactoperoxidase antibody is useful for Western blot analysis where a band is seen at ~100 kDa. Caution, do not use milk for the blocking or dilution buffers. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
100 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Human, monkey and bovine. Immunogen has 81% homology to sheep and 73% homology to mouse and rat.

Packaging, Storage & Formulations

Storage
Store at 4C in the dark.
Buffer
PBS
Preservative
0.05% Sodium Azide
Purity
Immunogen affinity purified

Notes

This conjugate is made on demand. Actual recovery may vary from the stated volume of this product. The volume will be greater than or equal to the unit size stated on the datasheet.

Alternate Names for Lactoperoxidase Antibody [FITC]

  • EC 1.11.1
  • lactoperoxidase
  • MGC129990
  • MGC129991
  • Salivary peroxidase
  • SAPX
  • SPOEC 1.11.1.7

Background

Lactoperoxidase (LPO) is a heme-containing glycoprotein enzyme of about 78 kD molecular size expressed highly in mammary gland, salivary gland and bronchial submucosal glands, and consequently found in large amounts in secretory fluids such as milk, tears, saliva, and lung fluids. Lactoperoxidase belongs to mammalian peroxidase family with a prosthetic heme group covalently linked to the proteins and other members of this family include myeloperoxidase (MPO), eosinophil peroxidase (EPO), and thyroid peroxidase (TPO). In the presence of H2O2, lactoperoxidase oxidizes thiocyanate (SCN-) more efficiently than other peroxidases, whereas MPO, EPO, and TPO most efficiently oxidize chloride, bromide, and iodide, respectively, to form hypo(pseudo)halides. After extracellular transport, SCN- gets oxidized either by peroxidases to form hypothiocyanite (OSCN-) or nonenzymatically by hypochlorite (OCl-). SCN exporters are the cystic fibrosis transmembrane conductance regulators, calcium-activated chloride channels, and pendrin/Slc26A4. OSCN- is tissue-innocuous but has a broad-spectrum microbicidal and microbistatic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Bioinformatics

Gene Symbol LPO