Lactoperoxidase Antibody [FITC]

• Reactivity: Hu, Bv, Pm
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: FITC

Lactoperoxidase Antibody [FITC] Summary

• Immunogen: Synthetic peptide made to an internal portion of the human Lactoperoxidase protein (within residues 300-400). [Swiss-Prot# P22079]
• Localization: Secreted, extracellular space.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: LPO
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This Lactoperoxidase antibody is useful for Western blot analysis where a band is seen at ~100 kDa. Caution, do not use milk for the blocking or dilution buffers. The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Theoretical MW: 100 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Sheep (81%).

Packaging, Storage & Formulations

• Storage: Store at 4C in the dark.
• Buffer: PBS
• Preservative: 0.05% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for Lactoperoxidase Antibody [FITC]

• EC 1.11.1
• lactoperoxidase
• MGC129990
• MGC129991
• Salivary peroxidase
• SAPX
• SPOEC 1.11.1.7

Background

Lactoperoxidase (LPO) is a heme-containing glycoprotein enzyme of about 78 kD molecular size expressed highly in mammary gland, salivary gland and bronchial submucosal glands, and consequently found in large amounts in secretory fluids such as milk, tears, saliva, and lung fluids. Lactoperoxidase belongs to mammalian peroxidase family with a prosthetic heme group covalently linked to the proteins and other members of this family include myeloperoxidase (MPO), eosinophil peroxidase (EPO), and thyroid peroxidase (TPO). In the presence of H2O2, lactoperoxidase oxidizes thiocyanate (SCN-) more efficiently than other peroxidases, whereas MPO, EPO, and TPO most efficiently oxidize chloride, bromide, and iodide, respectively, to form hypo(pseudo)halides. After extracellular transport, SCN- gets oxidized either by peroxidases to form hypothiocyanite (OSCN-) or nonenzymatically by hypochlorite (OCl-). SCN exporters are the cystic fibrosis transmembrane conductance regulators, calcium-activated chloride channels, and pendrin/Slc26A4. OSCN- is tissue-innocuous but has a broad-spectrum microbicidal and microbistatic activity.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: LPO