L-Selectin/CD62L Antibody (DREG56) - BSA Free

Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.

Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.

• Reactivity: Hu
• Applications: WB, Flow, IHC, IHC-Fr, IP, CyTOF-ready
• Clone: DREG56
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

L-Selectin/CD62L Antibody (DREG56) - BSA Free Summary

• Immunogen: PMA-activated human peripheral blood leukocytes
• Specificity: The mouse monoclonal antibody DREG56 recognizes CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells. HLDA V; WS Code S056
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: SELL
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • Flow Cytometry 2 ug/ml
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
  • Western Blot 1:1000
• Application Notes: Western Blot: non-reducing conditions Functional: Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV. This antibody is CyTOF ready.
• Theoretical MW: 76 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at 4C. Do not freeze.
• Buffer: PBS (pH 7.4)
• Preservative: 15mM Sodium Azide
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for L-Selectin/CD62L Antibody (DREG56) - BSA Free

• CD62L antigen
• CD62L
• gp90-MEL
• hLHRc
• LAM1
• LAM-1
• LAM1LECAM1
• LECAM1
• LEU8
• Leu-8
• Leukocyte adhesion molecule 1
• Leukocyte surface antigen Leu-8
• Leukocyte-endothelial cell adhesion molecule 1
• LNHR
• LNHRTQ1
• LSEL
• L-Selectin
• LYAM1
• Lyam-1
• LYAM1CD62 antigen-like family member L
• Lymph node homing receptor
• lymphocyte adhesion molecule 1
• pln homing receptor
• PLNHR
• selectin L
• SELL
• TQ1

Background

L-selectin, also known as CD62L, is a type I transmembrane glycoprotein that is primarily expressed on leukocytes and has a role in cell adhesion and migration (1,2). L-selectin is closely related to the other family members E- and P-selectin (1,2). Human L-selectin protein is encoded by SELL and is 372 amino acids (aa) in length with a predicted molecular weight (MW) of ~30 kDa (1,2). However, due to glycosylation the observed MW ranges between 65-100 kDa and glycosylation is cell-type dependent (1,2). The L-selectin protein contains an N-terminal C-type lectin domain (CTLD), an epidermal growth factor (EGF)-like domain, two sequence consensus repeat (CSR) domains, a cleavage site, a transmembrane (TM) domain, and a short cytoplasmic tail (1,2).

L-selectin expressed on leukocytes binds to ligands expressed by endothelial cells where it plays a role in lymphocyte homing to secondary lymphoid organs (2-5). L-selectin specifically recognizes and binds to sulfated sialyl-Lewis epitopes of O-linked glycans (2-4). Ligands for L-selectin include glycosylation-dependent cell adhesion molecule-1 (GlyCAM-1), CD34, mucosal vascular addressin cell adhesion molecule-1 (MAdCAM-1), and P-selectin glycoprotein ligand-1 (PSGL-1) (2,4). Elevated levels of selectin ligands on tumor cells are associated with cancer progression and metastasis (3). High levels of L-selectin and soluble L-selectin (sL-selectin) has been implicated in a number of pathologies from viral infection and allergies, to sepsis and multiple sclerosis (2,4,5). For example, L-selectin has been shown to play a role in human immunodeficiency virus (HIV) infection. HIV envelope glycans, such as gp120, binds to L-selectin/CD62L on CD4+ T cells, facilitating viral adhesion (2,5). A disintegrin and metalloproteinase (ADAM)17 is the primary enzyme responsible for L-selectin shedding in leukocytes, which is triggered in response to inflammatory signals (1,2,5). AMAD17 inhibitors block L-selectin shedding and reduce viral release (2,5). Given their role in cancer and other diseases, selectins and their ligands are potential targets for therapeutic intervention (3,5). For instance, murine models have shown that anti-L-selectin antibodies can delay onset of graft versus host disease (5).

References

1. Ivetic A. (2018). A head-to-tail view of L-selectin and its impact on neutrophil behaviour. Cell and Tissue Research, 371(3), 437-453. https://doi.org/10.1007/s00441-017-2774-x

2. Ivetic, A., Hoskins Green, H. L., & Hart, S. J. (2019). L-selectin: a major regulator of leukocyte adhesion, migration and signaling. Frontiers in Immunology, 10, 1068. https://doi.org/10.3389/fimmu.2019.01068

3. Borsig L. (2018). Selectins in cancer immunity. Glycobiology, 28(9), 648-655. https://doi.org/10.1093/glycob/cwx105

4. Kneuer, C., Ehrhardt, C., Radomski, M. W., & Bakowsky, U. (2006). Selectins-potential pharmacological targets?. Drug Discovery Today, 11(21-22), 1034-1040. https://doi.org/10.1016/j.drudis.2006.09.004

5. Segura, J., He, B., Ireland, J., Zou, Z., Shen, T., Roth, G., & Sun, P. D. (2021). The role of L-Selectin in HIV infection. Frontiers in Microbiology, 12, 725741. https://doi.org/10.3389/fmicb.2021.725741

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for L-Selectin/CD62L Antibody (NBP1-42795). (Showing 1 - 1 of 1 FAQ).

1. Is this antibody (NBP1-42795) validated for IHC Paraffin sections?
   • We have only validated our L-selectin Antibody (NBP1-42795) in IHC for frozen sections. We are unaware of its performance for paraffin embedded sections. However, I would recommend our Innovator's Reward program for your customer. Please contact us at innovators@novusbio.com for additional information on this program which gives an opportunity to the researches to test a product for an untested application/species.

Bioinformatics

• Gene Symbol: SELL