L-Selectin/CD62L Antibody (DREG56)

Images

 
Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the PE/Cy7 conjugate of NBP1-42795. Staining of normal human peripheral blood cells with Anti-Human CD4 FITC and Mouse IgG1 ? Isotype ...read more
Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the DyLight 405 conjugate of NBP1-42795. Staining of L-Selectin in human PBMCs using anti-L-Selectin antibody. Image from verified ...read more
Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the FITC conjugate of NBP1-42795. Staining of normal human peripheral blood cells with Anti-Human CD4 APC and Mouse IgG1 K Isotype Control ...read more
Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the PE conjugate of NBP1-42795. See Notes
Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the PE conjugate of NBP1-42795. Staining of L-Selectin in human PBMC using anti-L-Selectin antibody. The primary antibody was used at a ...read more
Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Analysis using the PE/Cy5 conjugate of NBP1-42795. Staining of normal human peripheral blood cells with PE-Cy5 Mouse IgG1 kappa Isotype Control (open ...read more

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, Flow, Func, IHC, IHC-Fr, IP, CyTOF-ready
Clone
DREG56
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated
Concentration
1 mg/ml

L-Selectin/CD62L Antibody (DREG56) Summary

Immunogen
PMA-activated human peripheral blood leukocytes
Specificity
The mouse monoclonal antibody DREG56 recognizes CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells. HLDA V; WS Code S056
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
SELL
Purity
Protein A purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:1000
  • Flow Cytometry 2 ug/ml
  • Functional
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:200
  • Immunoprecipitation 1:50
  • CyTOF-ready
Application Notes
Western Blot: non-reducing conditions Functional: Particularly effective in blocking of lymphocyte CD62L-mediated binding to peripheral lymph node HEV

Packaging, Storage & Formulations

Storage
Store at 4C. Do not freeze.
Buffer
PBS (pH 7.4)
Preservative
15mM Sodium Azide
Concentration
1 mg/ml
Purity
Protein A purified

Alternate Names for L-Selectin/CD62L Antibody (DREG56)

  • CD62L antigen
  • CD62L
  • gp90-MEL
  • hLHRc
  • LAM1
  • LAM-1
  • LAM1LECAM1
  • LECAM1
  • LEU8
  • Leu-8
  • Leukocyte adhesion molecule 1
  • Leukocyte surface antigen Leu-8
  • Leukocyte-endothelial cell adhesion molecule 1
  • LNHR
  • LNHRTQ1
  • LSEL
  • L-Selectin
  • LYAM1
  • Lyam-1
  • LYAM1CD62 antigen-like family member L
  • Lymph node homing receptor
  • lymphocyte adhesion molecule 1
  • pln homing receptor
  • PLNHR
  • selectin L
  • SELL
  • TQ1

Background

CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol

FAQs for L-Selectin/CD62L Antibody (NBP1-42795). (Showing 1 - 1 of 1 FAQ).

  1. Is this antibody (NBP1-42795) validated for IHC Paraffin sections?
    • We have only validated our L-selectin Antibody (NBP1-42795) in IHC for frozen sections. We are unaware of its performance for paraffin embedded sections. However, I would recommend our Innovator's Reward program for your customer. Please contact us at innovators@novusbio.com for additional information on this program which gives an opportunity to the researches to test a product for an untested application/species.

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NBP1-42795AF405
Alexa Fluor 488 Labeled NBP1-42795AF488
Alexa Fluor 647 Labeled NBP1-42795AF647
Alexa Fluor 700 Labeled NBP1-42795AF700
Allophycocyanin Labeled NBP1-42795APC
DyLight 350 Labeled NBP1-42795UV
DyLight 405 Labeled NBP1-42795V
DyLight 488 Labeled NBP1-42795G
DyLight 680 Labeled NBP1-42795FR
DyLight 755 Labeled NBP1-42795IR
FITC Labeled NBP1-42791
PE Labeled NBP1-42795PE
PerCP Labeled NBP1-42795PCP

Additional L-Selectin/CD62L Products

Bioinformatics Tool for L-Selectin/CD62L Antibody (NBP1-42795)

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Diseases for L-Selectin/CD62L Antibody (NBP1-42795)

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Pathways for L-Selectin/CD62L Antibody (NBP1-42795)

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PTMs for L-Selectin/CD62L Antibody (NBP1-42795)

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Research Areas for L-Selectin/CD62L Antibody (NBP1-42795)

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Blogs on L-Selectin/CD62L.

L-selectin (CD62L antigen, Leukocyte surface antigen Leu-8)
L-selectin is a member of the selectin family of glycoprotein adhesion and homing receptors that recognize sialyated carbohydrate groups and regulate lymphocyte-endothelial cell interactions. It is a type I transmembrane cell adhesion molecule (CAM) a...  Read full blog post.

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Bioinformatics

Gene Symbol SELL
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