FAB100A) and either (A) Mouse Anti-Human L‑Selectin/CD62L Monoclonal Antibody (Catalog # BBA24) or (B) isotype control antibody (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " alt="PBMC lymphocytes were stained with Mouse Anti-Human CD3 epsilon APC‑conjugated Monoclonal Antibody (Catalog # FAB100A) and either (A) Mouse Anti-Human L‑Selectin/CD62L Monoclonal Antibody (Catalog # BBA24) or (B) isotype control antibody (Catalog # MAB002) followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins. " class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB9787P-100 | |||
| Carrier Free | FAB9787U-100UG | |||
| Carrier Free | FAB9787V-100UG | |||
| Carrier Free | FAB9787N-100UG | |||
| Carrier Free | FAB9787S-100UG | |||
| Carrier Free | FAB9787G-100UG | |||
| Carrier Free | FAB9787R-100 | |||
| Carrier Free | FAB9787T-100 | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB100-65388-0.025mg | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF728-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP2-52556-0.025mg | |||
576-LS) was serially diluted and captured by Rat Anti-Mouse L‑Selectin/CD62L Monoclonal Antibody (Catalog # MAB5762) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse L‑Selectin/CD62L Monoclonal Antibody (Catalog # MAB5761) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse L‑Selectin/CD62L Fc Chimera (Catalog # 576-LS) was serially diluted and captured by Rat Anti-Mouse L‑Selectin/CD62L Monoclonal Antibody (Catalog # MAB5762) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse L‑Selectin/CD62L Monoclonal Antibody (Catalog # MAB5761) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB5762-SP | |||
| Carrier Free | FAB5762V-100UG | |||
| Carrier Free | FAB5762U-100UG | |||
| Carrier Free | FAB5762T-100UG | |||
| Carrier Free | FAB5762S-100UG | |||
| Carrier Free | FAB5762R-100UG | |||
| Carrier Free | FAB5762N-100UG | |||
| Carrier Free | FAB5762G-100UG | |||
CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." alt="L‑Selectin/CD62L was detected in perfusion fixed frozen sections of rat thymus using 15 µg/mL Goat Anti-Rat L‑Selectin/CD62L Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1534) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | AF1534-SP | |||
576-LS) was serially diluted and captured by Rat Anti-Mouse L‑Selectin/CD62L Monoclonal Antibody (Catalog # MAB5762) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse L‑Selectin/CD62L Monoclonal Antibody (Catalog # MAB5761) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Mouse L‑Selectin/CD62L Fc Chimera (Catalog # 576-LS) was serially diluted and captured by Rat Anti-Mouse L‑Selectin/CD62L Monoclonal Antibody (Catalog # MAB5762) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rat Anti-Mouse L‑Selectin/CD62L Monoclonal Antibody (Catalog # MAB5761) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | FAB5761F | |||
| Carrier Free | MAB5761-SP | |||
| Carrier Free | FAB5761V-100UG | |||
| Carrier Free | FAB5761U-100UG | |||
| Carrier Free | FAB5761T-100UG | |||
| Carrier Free | FAB5761S-100UG | |||
| Carrier Free | FAB5761R-100UG | |||
| Carrier Free | FAB5761N-100UG | |||
| Carrier Free | FAB5761G-100UG | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB1534-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB576-SP | |||
| Carrier Free | FAB576V-100UG | |||
| Carrier Free | FAB576U-100UG | |||
| Carrier Free | FAB576T-100UG | |||
| Carrier Free | FAB576S-100UG | |||
| Carrier Free | FAB576R-100UG | |||
| Carrier Free | FAB576N-100UG | |||
| Carrier Free | FAB576G-100UG | |||
F0101B) and Mouse anti-Human PE-conjugated CD3 Monoclonal Antibody (FAB100P). Quadrant markers were set based on isotype control antibody staining (MAB003, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." alt="Human PBMC either (A) resting or (B) treated with 100 ng/ml PMA for 24 hr, were stained with Mouse anti-Human L-Selectin/CD62 monoclonal antibody (Catalog # MAB728) followed by Allophycocyanin-conjugated anti-Mouse IgG Secondary Antibody (F0101B) and Mouse anti-Human PE-conjugated CD3 Monoclonal Antibody (FAB100P). Quadrant markers were set based on isotype control antibody staining (MAB003, data not shown). Staining was performed using our Staining Membrane-Associated Proteins protocol." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB728-SP | |||
| Carrier Free | FAB728V-100UG | |||
| Carrier Free | FAB728U-100UG | |||
| Carrier Free | FAB728T-100UG | |||
| Carrier Free | FAB728S-100UG | |||
| Carrier Free | FAB728R-100UG | |||
| Carrier Free | FAB728N-100UG | |||
| Carrier Free | FAB728G-100UG | |||
ADP2) was serially diluted and captured by Mouse Anti-Human L‑Selectin/CD62L Monoclonal Antibody (Catalog # BBA24) coated on a Clear Polystyrene Microplate (Catalog # ![Flow Cytometry: L-Selectin/CD62L Antibody (FMC46) [NB100-65388] - Flow Cytometry: L-Selectin/CD62L Antibody (FMC46) [PerCP] [NB100-65388PCP] - A cell surface stain was performed on human peripheral blood lymphocytes with L-Selectin/CD62L (FMC46) antibody NB100-65388PCP (blue) and a matched isotype control NBP2-27231PCP (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex. Image using the PerCP form of this antibody.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-FMC46-Flow-Cytometry-NB100-65388-img0003.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (FMC46) [NB100-65388] - Flow Cytometry: L-Selectin/CD62L Antibody (FMC46) [PerCP] [NB100-65388PCP] - A cell surface stain was performed on human peripheral blood lymphocytes with L-Selectin/CD62L (FMC46) antibody NB100-65388PCP (blue) and a matched isotype control NBP2-27231PCP (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Peridinin-Chlorophyll-Protein complex. Image using the PerCP form of this antibody.")
![Flow Cytometry: L-Selectin/CD62L Antibody (FMC46) [NB100-65388] - Staining of human peripheral blood granulocytes with Mouse anti Human CD62L:FITC](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-FMC46-Flow-Cytometry-NB100-65388-img0002.jpg)
ADP2) was serially diluted and captured by Mouse Anti-Human L‑Selectin/CD62L Monoclonal Antibody (Catalog # ![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0003.jpg "Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against human SELL (AA: 83-186) recombinant protein. (Expected MW is 37.4 kDa)")
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of MCF-7 cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0007.jpg)
![Western Blot: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis using SELL mAb against HEK293 (1) and SELL (AA:83-186)-hIgGFc transfected HEK293 (2) cell lysate.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Western-Blot-NBP2-52556-img0004.jpg)
![Immunocytochemistry/Immunofluorescence: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Analysis of Hela cells using SELL mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor- 555 phalloidin.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-Immunocytochemistry-Immunofluorescence-NBP2-52556-img0006.jpg)
![ELISA: L-Selectin/CD62L Antibody (8C8B7) [NBP2-52556] - Black line: Control Antigen (100 ng); Purple line: Antigen(10ng); Blue line: Antigen (50 ng); Red line: Antigen (100 ng);](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-8C8B7-ELISA-NBP2-52556-img0005.jpg)
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG56-Flow-Cytometry-NBP1-42795-img0015.jpg "Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Surface staining pattern of human peripheral blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.")
![Flow Cytometry: L-Selectin/CD62L Antibody (DREG56) [NBP1-42795] - Separation of human CD62L positive lymphocytes (red-filled) from CD62L negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD62L (DREG56) purified antibody (concentration in sample 1 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/L-Selectin-CD62L-Antibody-DREG56-Flow-Cytometry-NBP1-42795-img0014.jpg)
