L-Selectin/CD62L Antibody (DREG56) [FITC] Summary
PMA-activated human peripheral blood leukocytes
The mouse monoclonal antibody DREG56 recognizes CD62L / L-selectin, a 65-76 kDa cell surface protein, expressed by neutrophils, monocytes, and subsets of T, B, and NK cells, that interacts with specific carbohydrates exposed on activated endothelial cells. HLDA V; WS Code S056
Protein A purified
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- Western Blot 1:100-1:2000
- Flow Cytometry
- Immunohistochemistry 1:10-1:500
- Immunohistochemistry-Frozen 1:10-1:500
- Immunoprecipitation 1:10-1:500
The reagent is designed for Flow Cytometry analysis of human blood cells using 20 ul reagent / 100 ul of whole blood or 10^6 cells in a suspension.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Packaging, Storage & Formulations
Store at 4C in the dark.
PBS and 0.2% (w/v) high grade BSA (protease free)
15mM Sodium Azide
Protein A purified
Store in the dark. Avoid prolonged exposure to light.
Alternate Names for L-Selectin/CD62L Antibody (DREG56) [FITC]
- CD62L antigen
- Leukocyte adhesion molecule 1
- Leukocyte surface antigen Leu-8
- Leukocyte-endothelial cell adhesion molecule 1
- LYAM1CD62 antigen-like family member L
- Lymph node homing receptor
- lymphocyte adhesion molecule 1
- pln homing receptor
- selectin L
CD62L (L-selectin) is an adhesion glycoprotein that is constitutively expressed on the cell surface of leukocytes and mediates their homing to inflammatory sites and peripheral lymph nodes by enabling rolling along the venular wall. CD62L is also involved in activation-induced neutrophil aggregation. Activation-dependent CD62L shedding, however, counteracts neutrophil rolling. CD62L has also signaling roles including enhance of chemokine receptor expression. Similarly to CD62P, the major ligand of CD62L is PSGL-1 (P-selectin glycoprotein ligand-1). The level of CD62L expression can be used to help distinguish naive T cells from effector/memory T cells.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed
for 1 year from date of receipt.
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