L-Selectin/CD62L Antibody (95218) [Biotin]

• Reactivity: Mu
• Applications: ELISA(Det)
• Clone: 95218
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Biotin
• Concentration: LYOPH

L-Selectin/CD62L Antibody (95218) [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse L‑Selectin/CD62L
  Trp39-Asn332
  Accession # P18337
• Specificity: Detects mouse L‑Selectin/CD62L in ELISAs and Western blots. In Western blots, no cross‑reactivity with recombinant human L-Selectin, recombinant mouse (rm) E-Selectin, or rmP-Selectin is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SELL
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.5-2.0 ug/mL
  • ELISA Standard (Matched Pair)
• Application Notes: ELISA Capture: Mouse L-Selectin/CD62L Antibody (Catalog # MAB5762)
  ELISA Detection: Mouse L-Selectin/CD62L Biotinylated Antibody (Catalog # BAM5761)
  Standard: Recombinant Mouse L-Selectin/CD62L Fc Chimera (Catalog # 576-LS)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.5 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for L-Selectin/CD62L Antibody (95218) [Biotin]

• CD62L antigen
• CD62L
• gp90-MEL
• hLHRc
• LAM1
• LAM-1
• LAM1LECAM1
• LECAM1
• LEU8
• Leu-8
• Leukocyte adhesion molecule 1
• Leukocyte surface antigen Leu-8
• Leukocyte-endothelial cell adhesion molecule 1
• LNHR
• LNHRTQ1
• LSEL
• L-Selectin
• LYAM1
• Lyam-1
• LYAM1CD62 antigen-like family member L
• Lymph node homing receptor
• lymphocyte adhesion molecule 1
• pln homing receptor
• PLNHR
• selectin L
• SELL
• TQ1

Background

L‑Selectin (Leukocyte Selectin; also known as CD62L, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL‑14 antigen, DREG, and lymph node homing receptor), a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L‑Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is 90‑100 kDa. Human and mouse L‑Selectin share 76% amino acid sequence homology. L‑Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L‑Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction involving ICAM-1 and VCAM-1 leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue. ELISA techniques have shown that detectable levels of soluble L‑Selectin are present in the biological fluids of apparently normal individuals. Levels of L‑Selectin may be elevated or lowered in subjects with a variety of pathological conditions.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for L-Selectin/CD62L Antibody (BAM5761)(1)

Publication using BAM5761

• Novobrantseva, T;Manfra, D;Ritter, J;Razlog, M;O'Nuallain, B;Zafari, M;Nowakowska, D;Basinski, S;Phennicie, RT;Nguyen, PA;Brehm, MA;Sazinsky, S;Feldman, I; Preclinical Efficacy of VTX-0811: A Humanized First-in-Class PSGL-1 mAb Targeting TAMs to Suppress Tumor Growth Cancers 2024-08-06 [PMID: 39199551] (ELISA Detection, N/A)

Bioinformatics

• Gene Symbol: SELL
• Uniprot:
  • P18337()