L-Selectin/CD62L Antibody (95218) [Fluorescein]

Mouse splenocytes were stained with Rat Anti-Mouse L-Selectin/CD62L Fluorescein-conjugated Monoclonal Antibody (Catalog # FAB5761F, filled histogram) or isotype control antibody (Catalog # IC006F, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Mu
• Applications: Flow
• Clone: 95218
• Clonality: Monoclonal
• Host: Rat
• Conjugate: Fluorescein

L-Selectin/CD62L Antibody (95218) [Fluorescein] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse L‑Selectin/CD62L
  Met1-Asn332
  Accession # P18337
• Specificity: Detects mouse L-Selectin/CD62L in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human L-Selectin, recombinant mouse (rm) E-Selectin, or rmP-Selectin is observed.
• Source: N/A
• Isotype: IgG2a
• Clonality: Monoclonal
• Host: Rat
• Gene: SELL
• Purity: Protein A or G purified from hybridoma culture supernatant
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8degrees C. Do not use past expiration date. Protect from light.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide
• Purity: Protein A or G purified from hybridoma culture supernatant

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for L-Selectin/CD62L Antibody (95218) [Fluorescein]

• CD62L antigen
• CD62L
• gp90-MEL
• hLHRc
• LAM1
• LAM-1
• LAM1LECAM1
• LECAM1
• LEU8
• Leu-8
• Leukocyte adhesion molecule 1
• Leukocyte surface antigen Leu-8
• Leukocyte-endothelial cell adhesion molecule 1
• LNHR
• LNHRTQ1
• LSEL
• L-Selectin
• LYAM1
• Lyam-1
• LYAM1CD62 antigen-like family member L
• Lymph node homing receptor
• lymphocyte adhesion molecule 1
• pln homing receptor
• PLNHR
• selectin L
• SELL
• TQ1

Background

L-Selectin (Leukocyte Selectin), also known as CD62L, LAM-1, LECAM-1, LECCAM-1, TQ1, Leu-8, MEL-14 antigen, DREG, and Lymph Node Homing Receptor, a member of the Selectin family, is a cell surface glycoprotein expressed constitutively on a wide variety of leukocytes. Two forms of L-Selectin have been reported, apparently arising as a result of post-translational modifications. The lymphocyte form shows an apparent molecular weight of 74 kDa, while the neutrophil form is 90-100 kDa. Human and mouse L-Selectin share 76% amino acid sequence homology. L-Selectin plays a role in the migration of lymphocytes into peripheral lymph nodes and sites of chronic inflammation, and of neutrophils into acute inflammatory sites. Acting in cooperation with P-Selectin and E-Selectin, L-Selectin mediates the initial interaction of circulating leukocytes with endothelial cells that produces a characteristic "rolling" of the leukocytes on the endothelium. This initial interaction involving ICAM-1 and VCAM-1 leads eventually to extravasation of the white blood cell through the blood vessel wall into the extracellular matrix tissue.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for L-Selectin/CD62L Antibody (FAB5761F)(1)

Publication using FAB5761F

• Guabiraba R, Besnard A, Menezes G, Secher T, Jabir M, Amaral S, Braun H, Lima-Junior R, Ribeiro R, Cunha F, Teixeira M, Beyaert R, Graham G, Liew F IL-33 targeting attenuates intestinal mucositis and enhances effective tumor chemotherapy in mice. Mucosal Immunol, 2014-01-15;7(5):1079-93. 2014-01-15 [PMID: 24424522] (Flow Cytometry, Mouse)

Bioinformatics

• Gene Symbol: SELL
• Uniprot:
  • P18337()