KLF10 Antibody (2Y1Q6)

Western Blot: KLF10 Antibody (2Y1Q6) [NBP3-16133] - Western blot analysis of extracts of various cell lines, using KLF10 Rabbit mAb (NBP3-16133) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

• Reactivity: Hu, Mu
• Applications: WB, ELISA
• Clone: 2Y1Q6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated

KLF10 Antibody (2Y1Q6) Summary

• Description: Novus Biologicals Rabbit KLF10 Antibody (2Y1Q6) (NBP3-16133) is a recombinant monoclonal antibody validated for use in WB and ELISA. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human KLF10 (Q13118). MLNFGASLQQTAEERMEMISERPKESMYSWNKTAEKSDFEAVEALMSMSCSWKSDFKKYVENRPVTPVSDLSEEENLLPGTPDFHTIPAFCLTPPYSPSD
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: KLF10
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Western Blot 1:500 - 1:1000
• Theoretical MW: 53 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.3), 50% glycerol, 0.05% BSA
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for KLF10 Antibody (2Y1Q6)

• EGRA
• EGR-alpha
• KLF10
• Krueppel-like factor 10
• Kruppel-like factor 10
• TGFB inducible early growth response
• TGFB-inducible early growth response protein 1
• TIEG1
• TIEG-1
• TIEG1TIEG
• Transforming growth factor-beta-inducible early growth response protein 1

Background

Transforming growth factor-beta inducible early gene 1 (TIEG1) is a member of the 3 zinc-finger family of Kruppel-like transcription factors. It is also known as Kruppel-like factor 10 (KLF10). TIEG1 functions as an inducer or repressor of gene transcription to regulate the actions of estrogen and TGFbeta signaling and influences the processes of proliferation, differentiation, and apoptosis. Alternative names for TIEG1 include EGR-alpha, EGRA, and TIEG.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: KLF10