ITK Antibody (5G6)

Images

 
Western Blot: ITK Antibody (5G6) [NBP2-37578] - Western blot analysis using ITK mouse mAb against Jurkat cell lysate.
Immunocytochemistry/ Immunofluorescence: ITK Antibody (5G6) [NBP2-37578] - Immunofluorescence analysis of Jurkat cells using ITK mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye.
Flow Cytometry: ITK Antibody (5G6) [NBP2-37578] - Flow cytometric analysis of Jurkat cells using ITK mouse mAb (blue) and negative control (red).
Western Blot: ITK Antibody (5G6) [NBP2-37578] - Western blot analysis using ITK mAb against human ITK (AA: 2-120) recombinant protein. (Expected MW is 39.7 kDa)
ELISA: ITK Antibody (5G6) [NBP2-37578] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF
Clone
5G6
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

ITK Antibody (5G6) Summary

Immunogen
Purified recombinant fragment of human ITK expressed in E. Coli.
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
ITK
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500 - 1:2000
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
Theoretical MW
72 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for ITK Antibody (5G6)

  • EC 2.7.10
  • EC 2.7.10.2
  • EMT
  • EMTMGC126257
  • homolog of mouse T-cell itk/tsk
  • IL2-inducible T-cell kinase
  • ITK
  • Kinase EMT
  • LYK
  • LYKMGC126258
  • PSCTK2
  • T-cell-specific kinase
  • Tcsk
  • tyrosine-protein kinase ITK/TSK
  • Tyrosine-protein kinase Lyk

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Product General Protocols

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Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol ITK