Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Non-transfected (-) and transfected (+) HepG2 whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with IDH1 antibody diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody

Western Blot: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - A. 30 ug Neuro2A whole cell extract B. 30 ug C8D30 whole cell extractC. 30 ug NIH-3T3 whole cell extract D. 30 ug Raw 264.7 whole cell extract E. 30 ug C2Cl2 whole cell extract 10 % SDS-PAGE IDH1 antibody dilution: 1:1000

Immunohistochemistry-Paraffin: Isocitrate dehydrogenase Antibody [NBP1-31599] - Paraffin-embedded colon ca, using antibody at 1:100 dilution.

Immunoprecipitation: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - HepG2 whole cell lysate/extract A : 30 ug whole cell lysate/extract of IDH1 protein expressing HepG2 cells B : Control with 2.5 ug of pre-immune rabbit IgG C : Immunoprecipitation of IDH-1 protein by 2.5 ug of IDH-1 antibody 10% SDS-PAGE The immunoprecipitated IDH-1 protein was detected by IDH-1 antibody diluted at 1 : 1000. EasyBlot anti-rabbit IgG (HRP) was used as a secondary reagent.

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat colon.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded rat duodenum.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse intestine.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse liver.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody [NBP1-31599] - Isocitrate Dehydrogenase 1/IDH1 antibody detects Isocitrate Dehydrogenase 1/IDH1 protein at cytoplasm by immunohistochemical analysis.Sample: Paraffin-embedded mouse brain.Isocitrate Dehydrogenase 1/IDH1 stained by Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000.Antigen Retrieval: Citrate buffer, pH 6.0, 15 min

Wild-type (WT) and Isocitrate Dehydrogenase 1/IDH1 knockout (KO) HeLa cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:500. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody, and the signal was developed with Trident ECL plus-Enhanced.

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Various tissue extracts (50 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with Isocitrate Dehydrogenase 1/IDH1 antibody (NBP1-31599) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

• Reactivity: Hu, Mu, Rt, RM
• Applications: WB, IHC, IP, KO, Mycoplasma
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free Summary

• Description: Novus Biologicals Knockout (KO) Validated Rabbit Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free (NBP1-31599) is a polyclonal antibody validated for use in IHC, WB and IP. Anti-Isocitrate Dehydrogenase 1/IDH1 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Recombinant protein encompassing a sequence within the center region of human Isocitrate Dehydrogenase 1/IDH1. The exact sequence is proprietary.
• Localization: Cytoplasm, Peroxisome
• Predicted Species: Rhesus Macaque (100%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IDH1
• Purity: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry 1:1000-1:10000
  • Immunohistochemistry-Paraffin 1:100-1:1000
  • Immunoprecipitation 1:100-1:1000
  • Knockdown Validated
  • Knockout Validated
  • Western Blot 1:1000-1:10000
• Theoretical MW: 47 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
• Buffer: PBS, 20% Glycerol
• Preservative: 0.025% Proclin 300
• Purity: Antigen Affinity-purified

Alternate Names for Isocitrate Dehydrogenase 1/IDH1 Antibody - BSA Free

• Cytosolic NADP-isocitrate dehydrogenase
• EC 1.1.1.42
• IDCD
• IDH
• IDH1
• IDP
• IDPC
• isocitrate dehydrogenase [NADP] cytoplasmic
• isocitrate dehydrogenase 1 (NADP+), soluble
• Isocitrate Dehydrogenase 1
• NADP(+)-specific ICDH
• NADP-dependent isocitrate dehydrogenase, cytosolic
• NADP-dependent isocitrate dehydrogenase, peroxisomal
• Oxalosuccinate decarboxylase
• PICD

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-31599)(1)

Publication using NBP1-31599

• de Oliveira B M, Matsumura C Y et al. Quantitative proteomic analysis reveals metabolic alterations, calcium dysregulation, and increased expression of extracellular matrix proteins in laminin alpha 2 chain-deficient muscle. Mol Cell Proteomics 2014-01-11 [PMID: 24994560] (WB, Mouse)

Review for Isocitrate Dehydrogenase 1/IDH1 Antibody (NBP1-31599) (1)

Average Rating: 5

(Based on 1 review)

We have 1 review tested in 1 species: Human.

reviewed by: Verified Customer WB Human 12/11/2023

Summary

• Application: Western Blot
• Sample Tested: fibroblasts
• Species: Human
• Lot: 43313

Bioinformatics

• Gene Symbol: IDH1