CEBP alpha Antibody (5B7)

Images

 
Western Blot: CEBP alpha Antibody (5B7) [NBP2-37497] - Western blot analysis using CEBPA mouse mAb against Jurkat (1), k562 (2), and HepG2 (3) cell lysate.
Immunocytochemistry/ Immunofluorescence: CEBP alpha Antibody (5B7) [NBP2-37497] - Immunofluorescence analysis of HeLa cells using CEBPA mouse mAb (green). Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been ...read more
Immunohistochemistry: CEBP alpha Antibody (5B7) [NBP2-37497] - Immunohistochemical analysis of paraffin-embedded rectum tissues using CEBPA mouse mAb with DAB staining.
Flow Cytometry: CEBP alpha Antibody (5B7) [NBP2-37497] - Flow cytometric analysis of MCF-7 cells using CEBPA mouse mAb (green) and negative control (red).
Immunocytochemistry/ Immunofluorescence: CEBP alpha Antibody (5B7) [NBP2-37497] - Immunofluorescence analysis of HeLa cells. Blue: DRAQ5 fluorescent DNA dye. Red: Actin filaments have been labeled with Alexa Fluor-555 ...read more
ELISA: CEBP alpha Antibody (5B7) [NBP2-37497] - Red: Control Antigen (100ng); Purple: Antigen (10ng); Green: Antigen (50ng); Blue: Antigen (100ng);

Product Details

Summary
Reactivity HuSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P
Clone
5B7
Clonality
Monoclonal
Host
Mouse
Conjugate
Unconjugated

Order Details

CEBP alpha Antibody (5B7) Summary

Immunogen
Synthesized peptide of human CEBPA (AA: C-RKSRDKAKRNVETKV).
Isotype
IgG1
Clonality
Monoclonal
Host
Mouse
Gene
CEBPA
Purity
Unpurified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • ELISA 1:10000
  • Flow Cytometry 1:200 - 1:400
  • Immunocytochemistry/Immunofluorescence 1:200 - 1:1000
  • Immunohistochemistry 1:200 - 1:1000
  • Immunohistochemistry-Paraffin 1:200 - 1:1000
  • Western Blot 1:500 - 1:2000
Theoretical MW
42 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
Ascites
Preservative
0.03% Sodium Azide
Purity
Unpurified

Alternate Names for CEBP alpha Antibody (5B7)

  • C/EBP alpha
  • C/EBP-alpha
  • CCAAT/enhancer binding protein (C/EBP), alpha
  • CCAAT/enhancer-binding protein alpha
  • CEBP alpha
  • CEBP
  • CEBPA

Background

CCAAT/enhancer-binding proteins (C/EBP) consist of a family of transcription factors that play an important role in regulating the balance between cell growth & differentiation. The various isoforms of C/EBP (alpha, beta, gamma, delta, epsilon, zeta) exhibit similar DNA-binding specificities and contain a leucine zipper dimerization domain (1). C/EBP alpha (2) can form heterodimers with related protein C/EBP beta and gamma. C/EBP alpha directly interacts with CDK2and CDK4 and arrests cell proliferation by inhibiting these kinases (3). C/EBP alpha has been shown to bind to the promoter and modulate the expression of leptin, a protein that plays an important role in body weight homeostasis (4). C/EBP alpha also has prominent antimitotic activity and may play a role of a tumor suppressor.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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ICC/IF Video Protocol

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Secondary Antibodies

 

Isotype Controls

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Blogs on CEBP alpha.

Transcription Factor Antibodies Used In Landmark Evolutionary Study
We at Novus Biologicals offer a full antibody database targeted to transcription factor research. Recently, CEBP antibodieswere used in a research study exploring the evolution of gene regulation in various vertebrates. The results revealed surprising...  Read full blog post.

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Bioinformatics

Gene Symbol CEBPA