MGMT Antibody (MT 23.2)


Western Blot: MGMT Antibody (MT 23.2) [NB100-168] - Analysis of MGMT (MT 23.2) antibody (NB100-168) - Detection of MGMT in MCF7 lysate using NB100-168 at 1:1000 dilution.
Immunocytochemistry/ Immunofluorescence: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT (Green) in Hela cells using NB100-168 at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunocytochemistry/ Immunofluorescence: MGMT Antibody (MT 23.2) [NB100-168] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were more
Simple Western: MGMT Antibody (MT 23.2) [NB100-168] - Simple Western lane view shows a specific band for MGMT in 0.2 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, Mu(-)Species Glossary
Applications WB, Simple Western, Flow, ICC/IF, IHC, IHC-P
MT 23.2
1.0 mg/ml

MGMT Antibody (MT 23.2) Summary

Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli [UniProt# P16455]
Protein G purified
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Test in a species/application not listed above to receive a full credit towards a future purchase.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified


Application Notes
This MGMT (MT 23.2) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, a band at ~24 kDa can be seen. In ICC/IF, strong staining has been seen in CEM cells and no signal has been seen in TK6 cells.In Simple Western only 10-15 uL of the recommended dilution is used per data point.

The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
24 kDa
Read Publications using
NB100-168 in the following applications:

  • IHC
    3 publications
  • 2 publications
  • WB
    8 publications

Reactivity Notes

Human. This antibody does not work in mouse.

Alternate Names for MGMT Antibody (MT 23.2)

  • 6-O-methylguanine-DNA methyltransferase
  • EC
  • methylated-DNA--protein-cysteine methyltransferase
  • methylguanine-DNA methyltransferase
  • O-6-methylguanine-DNA methyltransferase
  • O6-methylguanine-DNA methyltransferase
  • O-6-methylguanine-DNA-alkyltransferase


MGMT (6-O-methylguanine-DNA methyltransferase) is a DNA repair enzyme with the ability to protect cells from carcinogenic effects of alkylating agents by removing adducts from the O(6) position of guanine. MGMT repairs alkylated guanine residues in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme, and in this so called 'suicidal reaction', the enzyme is irreversibly inactivated. Localized in the nucleus, MGMT is ubiquitously expressed in normal human tissues with overexpression observed in several types of cancerous cells wherein it provides resistance against alkylating drugs. Howeverit is found epigenetically silenced in some cancers that include lymphoma, breast and prostate cancer, as well as retinoblastoma, wherein this silencing is thought to be due to promoter methylation which interferes with transcriptional factors binding. Indeed, MGMT silencing has been linked to mutations in other tumor-related genes including silencing of p53 (non-small cell lung cancer and astrocytic tumors), k-ras gene mutations (gastric and colorectal cancer), and methylation of CDKN1A gene (encodes p21) as well as CDKN2A gene (encodes p16).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MGMT Antibody (NB100-168)(15)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NB100-168 Applications Species
Oktay Y, ulgen E, Can O et al. IDH-mutant glioma specific association of rs55705857 located at 8q24.21 involves MYC deregulation. Sci Rep Jun 10 2016 12:00AM [PMID:27282637] (IHC) IHC
Oktay Y, ulgen E, Can O et al. IDH-mutant glioma specific association of rs55705857 located at 8q24.21 involves MYC deregulation. Sci Rep 2016 Jun 10 [PMID:27282637] (IHC) IHC
Fang Q, Inanc B, Schamus S et al. HSP90 regulates DnA repair via the interaction between XRCC1 and DnA polymerase B. Nat Commun. 2014 Nov 26 [PMID:25423885] (WB) WB
Kewitz S, Stiefel M, Kramm CM, Staege MS. Impact of O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation and MGMT expression on dacarbazine resistance of Hodgkin's lymphoma cells. Leuk Res. 2013 Nov 13 [PMID:24284332] (WB, Human) WB Human
Persano L, Pistollato F, Rampazzo E et al. BMP2 sensitizes glioblastoma stem-like cells to Temozolomide by affecting HIF-1 alpha stability and MGMT expression Cell Death Dis 2012 Oct 18 [PMID:23076220] (WB, Human) WB Human
Lylo, V V, Matsevich L L, Kotsarenko, E V et al. Activation of Gene Expression of the O6 Methylguanine DNA Transferase Repair Enzyme upon the Influence of EMAP II Cytokine in Human Cells In Vitro. Cytology and Genetics 2011 [PMID:22329163]
Herzig MC, Hildreth K, Huamani J, Perez M, Goins BA, McMahan CA, Reddick RL, Walter CA. Human O(6) -methylguanine-DNA methyltransferase containing C145A does not prevent hepatocellular carcinoma in C3HeB/FeJ transgenic mice. Mol Carcinog. 2011 Dec 27. [PMID:22213062] (WB, Mouse) WB Mouse
Della Puppa A, Persano L, Masi G et al. MGMT expression and promoter methylation status may depend on the site of surgical sample collection within glioblastoma: a possible pitfall in stratification of patients?. J Neurooncol. 2011 Jul 2. [PMID:21725802]
Tang JB, Svilar D, Trivedi RN, Wang XH, Goellner EM, Moore B, Hamilton RL, Banze LA, Brown AR, Sobol RW. N-methylpurine DNA glycosylase and DNA polymerase beta modulate BER inhibitor potentiation of glioma cells to temozolomide. Neuro Oncol;13(5):471-86. 2011 May. [PMID:21377995] (WB, Human) WB Human
Pistollato F, Abbadi S, Rampazzo E et al. Intratumoral hypoxic gradient drives stem cells distribution and MGMT expression in glioblastoma. Stem Cells 2010 May [PMID:20309962] (IHC-P, WB, Human) IHC-P, WB Human
Show All 15 Publications.

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Product General Protocols

View specific protocols for MGMT Antibody (NB100-168): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

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