MGMT Antibody (MT 23.2)


Western Blot: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT in MCF7 lysate using NB100-168 at 1:1000 dilution.
Immunocytochemistry/ Immunofluorescence: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT (Green) in Hela cells using NB100-168 at a 1:50 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.
Immunohistochemistry-Paraffin: MGMT Antibody (MT 23.2) [NB100-168] - IHC analysis of a formalin fixed paraffin-embedded (FFPE) human kidney using 1:2000 conc. of MGMT antibody (clone MT 23.2) on a Bond Rx autostainer more
Flow Cytometry: MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on HeLa cells with MGMT Antibody (MT 23.2) antibody NB100-168 (blue) and a matched isotype control (orange). Cells were fixed more
Immunocytochemistry/ Immunofluorescence: MGMT Antibody (MT 23.2) [NB100-168] - MCF7 cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The cells were more
Flow (Intracellular): MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on Jurkat cells with MGMT Antibody (MT 23.2) antibody NB100-168 (blue) and a matched isotype control NBP2-27231 (orange). more
Flow Cytometry: MGMT Antibody (MT 23.2) [NB100-168] - Detection of MGMT in Human Jurkat Cell Line by Flow Cytometry. Human Jurkat cell line was stained with Mouse Anti- MGMT Monoclonal Antibody (Catalog # NB100-168, more
Flow (Intracellular): MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on Raji cells with MGMT Antibody (MT 23.2) antibody NB100-168AF647 (blue) and a matched isotype control NBP2-27231AF647 more
Flow (Intracellular): MGMT Antibody (MT 23.2) [NB100-168] - An intracellular stain was performed on Jurkat cells with MGMT Antibody (MT 23.2) antibody NB100-168AF647 (blue) and a matched isotype control (orange). Cells more
Simple Western: MGMT Antibody (MT 23.2) [NB100-168] - Simple Western lane view shows a specific band for MGMT in 0.2 mg/ml of MCF-7 lysate. This experiment was performed under reducing conditions using the 12-230 kDa more

Product Details

Reactivity Hu, Mu(-)Species Glossary
Applications WB, Simple Western, Flow, Flow-IC, ICC/IF, IHC, IHC-P, CyTOF-ready
MT 23.2
1.0 mg/ml

Order Details

MGMT Antibody (MT 23.2) Summary

Recombinant human MGMT (O6-methylguanine-DNA methyltransferase) purified from E. coli [UniProt# P16455]
Protein G purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Western Blot 1:1000
  • Simple Western 1:1000
  • Flow Cytometry 1-2 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:500
  • Immunohistochemistry 1:500-1000
  • Immunohistochemistry-Paraffin 1:500-1:1000
  • CyTOF-ready
  • Flow (Intracellular)
Application Notes
This MGMT (MT 23.2) antibody is useful for Flow Cytometry, Immunocytochemistry/Immunofluorescence, Immunohistochemistry on paraffin-embedded sections and Western Blot. In WB, a band at approx. 24 kDa can be seen. In ICC/IF, strong staining has been seen in CEM cells and no signal has been seen in TK6 cells.

In Simple Western only 10 - 15 ul of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
24 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
NB100-168 in the following applications:

  • IHC
    3 publications
  • 2 publications
  • WB
    9 publications

Reactivity Notes

Human. This antibody is mouse reactivity negative.

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
1.0 mg/ml
Protein G purified

Alternate Names for MGMT Antibody (MT 23.2)

  • 6-O-methylguanine-DNA methyltransferase
  • EC
  • methylated-DNA--protein-cysteine methyltransferase
  • methylguanine-DNA methyltransferase
  • MGMT
  • O-6-methylguanine-DNA methyltransferase
  • O6-methylguanine-DNA methyltransferase
  • O-6-methylguanine-DNA-alkyltransferase


MGMT (6-O-methylguanine-DNA methyltransferase) is a DNA repair enzyme with the ability to protect cells from carcinogenic effects of alkylating agents by removing adducts from the O(6) position of guanine. MGMT repairs alkylated guanine residues in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme, and in this so called 'suicidal reaction', the enzyme is irreversibly inactivated. Localized in the nucleus, MGMT is ubiquitously expressed in normal human tissues with overexpression observed in several types of cancerous cells wherein it provides resistance against alkylating drugs. However, it is found epigenetically silenced in some cancers that include lymphoma, breast and prostate cancer, as well as retinoblastoma, wherein this silencing is thought to be due to promoter methylation which interferes with transcriptional factors binding. MGMT silencing has been linked to mutations in other tumor-related genes including silencing of p53 (non-small cell lung cancer and astrocytic tumors), k-ras gene mutations (gastric and colorectal cancer), and methylation of CDKN1A gene (encodes p21) as well as CDKN2A gene (encodes p16).


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for MGMT Antibody (NB100-168)(18)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, WB.

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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using NB100-168 Applications Species
KotSarenko K, Lylo V, Ruban T et al. Effects of Some Growth Factors and Cytokines on the Expression of the Repair Enzyme MGMT and Protein MARP in Human Cells In Vitro : Effect of Some Growth Factors and Cytokines. Biochem. Genet. Mar 27 2018 [PMID: 29589213] (Human) Human
Altinoz MA, Elmaci I, Bolukbasi FH et al. MGMT gene variants, temozolomide myelotoxicity and glioma risk. A concise literature survey including an illustrative case. J Chemother. Apr 23 2017 [PMID: 28436299] (Human) Human
Oktay Y, ulgen E, Can O et al. IDH-mutant glioma specific association of rs55705857 located at 8q24.21 involves MYC deregulation. Sci Rep Jun 10 2016 [PMID: 27282637] (IHC) IHC
Stepanenko AA, Andreieva SV, Korets KV et al. Temozolomide promotes genomic and phenotypic changes in glioblastoma cells. Cancer Cell Int 2016 May 05 [PMID: 27158244] (WB) WB
Fang Q, Inanc B, Schamus S et al. HSP90 regulates DnA repair via the interaction between XRCC1 and DnA polymerase B. Nat Commun. 2014 Nov 26 [PMID: 25423885] (WB) WB
Kewitz S, Stiefel M, Kramm CM, Staege MS. Impact of O6-methylguanine-DNA methyltransferase (MGMT) promoter methylation and MGMT expression on dacarbazine resistance of Hodgkin's lymphoma cells. Leuk Res. 2013 Nov 13 [PMID: 24284332] (WB, Human) WB Human
Persano L, Pistollato F, Rampazzo E et al. BMP2 sensitizes glioblastoma stem-like cells to Temozolomide by affecting HIF-1 alpha stability and MGMT expression Cell Death Dis 2012 Oct 18 [PMID: 23076220] (WB, Human) WB Human
Lylo, V V, Matsevich L L, Kotsarenko, E V et al. Activation of Gene Expression of the O6 Methylguanine DNA Transferase Repair Enzyme upon the Influence of EMAP II Cytokine in Human Cells In Vitro. Cytology and Genetics 2011 [PMID: 22329163]
Herzig MC, Hildreth K, Huamani J, Perez M, Goins BA, McMahan CA, Reddick RL, Walter CA. Human O(6) -methylguanine-DNA methyltransferase containing C145A does not prevent hepatocellular carcinoma in C3HeB/FeJ transgenic mice. Mol Carcinog. 2011 Dec 27. [PMID: 22213062] (WB, Mouse) WB Mouse
Doll C, Craighead P, Lees-Miller S, Demetrick D, Eliasziw M, Magliocco A. Expression of Mgmt in Cervical Cancer: a Potential Biomarker for Response to Radiation in Cervical Cancer. 21st Annual Scientific Meeting of the Canadian Association of Radiation Oncologists joint with Canadian Organization of Medical; Toronto; Canada. 9-Oct-07.
Show All 18 Publications.

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Product General Protocols

View specific protocols for MGMT Antibody (NB100-168): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for MGMT Antibody (NB100-168) (0)

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Secondary Antibodies


Isotype Controls

Other Available Formats

Alexa Fluor 350 NB100-168AF350
Alexa Fluor 405 NB100-168AF405
Alexa Fluor 488 NB100-168AF488
Alexa Fluor 532 NB100-168AF532
Alexa Fluor 594 NB100-168AF594
Alexa Fluor 647 NB100-168AF647
Alexa Fluor 700 NB100-168AF700
Alexa Fluor 750 NB100-168AF750
Allophycocyanin NB100-168APC
Allophycocyanin/Cy7 NB100-168APCCY7
Biotin NB100-168B
DyLight 350 NB100-168UV
DyLight 405 NB100-168V
DyLight 488 NB100-168G
DyLight 550 NB100-168R
DyLight 594 NB100-168DL594
DyLight 650 NB100-168C
DyLight 680 NB100-168FR
DyLight 755 NB100-168IR
FITC NB100-168F
HRP NB100-168H
Janelia Fluor 549 NB100-168JF549
Janelia Fluor 646 NB100-168JF646
PE NB100-168PE
PE/Atto594 NB100-168PEATT594
PE/Cy5.5 NB100-168PECY55
PE/Cy7 NB100-168PECY7
PerCP NB100-168PCP

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Gene Symbol MGMT