IDH2 Antibody

Images

 
Western Blot: IDH2 Antibody [NBP2-22166] - 1) Non transfected 293T cells. 2) IDH2-flag (c-term) transfected 293T cells. WB image submitted by a verified customer review.
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody [NBP2-22166] - 293T cells transfected with IDH2-flag expression construct were fixed and stained with anti-IDH2 antibody followed by Alexa Fluor 488 anti-rabbit ...read more
Immunohistochemistry: IDH2 Antibody [NBP2-22166] - Staining of IDH2 in mouse intestine.
Western Blot: IDH2 Antibody [NBP2-22166] - Analysis of Caki-1 cell lysate (A), human skeletal muscle tissue (B), mouse skeletal muscle tissue (C), and rat skeletal muscle tissue (D) using IDH2 antibody at a ...read more
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody [NBP2-22166] - IDH2 antibody was tested in U2OS cells with DyLight (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight 550 (red).

Product Details

Summary
Reactivity Hu, Mu, Rt, Gp, Ha, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

IDH2 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human IDH2 protein (between residues 375-452) [UniProt P48735].
Localization
Mitochondria
Predicted Species
Guinea Pig (100%), Rabbit (100%), Hamster (100%), Primate (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IDH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1 - 2 ug/mL
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
Application Notes
In Western blot a band is observed ~48 kDa. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Control
Human Skeletal Muscle Whole Tissue Lysate (Adult Whole Normal)
Control Peptide
IDH2 Peptide (NBP2-22166PEP)
Reviewed Applications
Read 2 Reviews rated 5
using
NBP2-22166 in the following applications:

Publications
Read Publications using
NBP2-22166 in the following applications:

  • WB
    3 publications

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IDH2 Antibody

  • D2HGA2
  • EC 1.1.1.42
  • ICD-M
  • IDH
  • IDHM
  • IDP
  • IDPM
  • isocitrate dehydrogenase [NADP], mitochondrial
  • isocitrate dehydrogenase 2 (NADP+), mitochondrial
  • mNADP-IDH
  • NADP(+)-specific ICDH
  • Oxalosuccinate decarboxylase

Background

Isocitrate dehydrogenase 2, also known as IDH, IDP, oxalosuccinate decarboxylase, IDPM, and mNADP-IDH, catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH2 is imperative for metabolic regulation and energy production, primarily via the citric acid cycle. In addition, IDH2 has been implicated in tight interactions with the pyruvate dehydrogenase complex. IDH2 is localized in the mitochondria and is found in stems, roots and leaves. Recent research has found that mutations in IDH2 can block histone demethylation and result in an inability for cells to differentiate in gliomas (PMID: 22343901). Further studies have also linked IDH2 to both gallbladder and bile duct cancer (PMID: 22180306).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP2-22166
Species: Hu, Mu, Rt, Gp, Ha, Pm, Rb
Applications: WB, ICC/IF, IHC, IHC-P

Publications for IDH2 Antibody (NBP2-22166)(4)

Reviews for IDH2 Antibody (NBP2-22166) (2) 52

Average Rating: 5
(Based on 2 reviews)
We have 2 reviews tested in 1 species: Human.

Reviews using NBP2-22166:
Filter by Applications
ICC
(1)
WB
(1)
All Applications
Filter by Species
Human
(2)
All Species
Images Ratings Applications Species Date Details
Immunocytochemistry IDH2 NBP2-22166
Enlarge
5
reviewed by:
Anonymous
ICC Human 01/02/2020
View

Summary

ApplicationImmunocytochemistry
Sample Tested293T
SpeciesHuman
LotA-1
Western Blot IDH2 NBP2-22166
Enlarge
5
reviewed by:
Anonymous
WB Human 01/02/2020
View

Summary

ApplicationWestern Blot
Sample Tested293T
SpeciesHuman
LotA-1

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IDH2 Antibody (NBP2-22166) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 350 NBP2-22166AF350
Alexa Fluor 405 NBP2-22166AF405
Alexa Fluor 488 NBP2-22166AF488
Alexa Fluor 532 NBP2-22166AF532
Alexa Fluor 594 NBP2-22166AF594
Alexa Fluor 647 NBP2-22166AF647
Alexa Fluor 700 NBP2-22166AF700
Alexa Fluor 750 NBP2-22166AF750
Biotin NBP2-22166B
DyLight 350 NBP2-22166UV
DyLight 405 NBP2-22166V
DyLight 488 NBP2-22166G
DyLight 550 NBP2-22166R
DyLight 594 NBP2-22166DL594
DyLight 650 NBP2-22166C
DyLight 680 NBP2-22166FR
DyLight 755 NBP2-22166IR
FITC NBP2-22166F
HRP NBP2-22166H
Janelia Fluor 549 NBP2-22166JF549
Janelia Fluor 646 NBP2-22166JF646

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Blogs on IDH2

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Contact Information

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Recent Reviews

5
5
2
4
0
3
0
2
0
1
0

Anonymous
01/02/2020
Application: ICC
Species: Human

Anonymous
01/02/2020
Application: WB
Species: Human

Bioinformatics

Gene Symbol IDH2
Entrez
Uniprot