Western Blot: IDH2 Antibody [NBP2-22166] - Western blot analysis of Caki-1 cell lysate (A), human skeletal muscle tissue (B), mouse skeletal muscle tissue (C), and rat skeletal muscle tissue (D) using IDH2 antibody at a ...read more
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody [NBP2-22166] - IDH2 antibody was tested in U2OS cells with DyLight (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: IDH2 Antibody [NBP2-22166] - IHC staining of IDH2 in mouse intestine.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
This IDH2 antibody is useful for Western blot, Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence. In Western blot a band was observed ~48 kDa. In ICC/IF mitochondrial staining was observed in U2Os cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Human, mouse and rat. Predicted to react with monkey, hamster, Guinea pig and rabbit based on 100% sequence homology.
Alternate Names for IDH2 Antibody
isocitrate dehydrogenase [NADP], mitochondrial
isocitrate dehydrogenase 2 (NADP+), mitochondrial
Isocitrate dehydrogenase 2, also known as IDH, IDP, oxalosuccinate decarboxylase, IDPM, and mNADP-IDH, catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH2 is imperative for metabolic regulation and energy production, primarily via the citric acid cycle. In addition, IDH2 has been implicated in tight interactions with the pyruvate dehydrogenase complex. IDH2 is localized in the mitochondria and is found in stems, roots and leaves. Recent research has found that mutations in IDH2 can block histone demethylation and result in an inability for cells to differentiate in gliomas (PMID: 22343901). Further studies have also linked IDH2 to both gallbladder and bile duct cancer (PMID: 22180306).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.