IDH2 Antibody

Images

 
Western Blot: IDH2 Antibody [NBP2-22166] - Western blot analysis of Caki-1 cell lysate (A), human skeletal muscle tissue (B), mouse skeletal muscle tissue (C), and rat skeletal muscle tissue (D) using IDH2 antibody at a ...read more
Immunocytochemistry/ Immunofluorescence: IDH2 Antibody [NBP2-22166] - IDH2 antibody was tested in U2OS cells with DyLight (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and Dylight 550 (red).
Immunohistochemistry: IDH2 Antibody [NBP2-22166] - IHC staining of IDH2 in mouse intestine.

Product Details

Summary
Reactivity Hu, Mu, Rt, GP, Ha, Pm, RbSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

Order Details

IDH2 Antibody Summary

Immunogen
A synthetic peptide made to a C-terminal portion of the human IDH2 protein (between residues 375-452) [UniProt P48735].
Localization
Mitochondria
Predicted Species
Guinea Pig (100%), Hamster (100%), Primate (100%), Rabbit (100%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IDH2
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-2 ug/ml
  • Immunocytochemistry/Immunofluorescence 1:250 - 1:500
  • Immunohistochemistry 1:100 - 1:500
  • Immunohistochemistry-Paraffin 1:100 - 1:500
Application Notes
This IDH2 antibody is useful for Western blot, Immunohistochemistry paraffin embedded sections and Immunocytochemistry/Immunofluorescence. In Western blot a band was observed ~48 kDa. In ICC/IF mitochondrial staining was observed in U2Os cells. Prior to immunostaining paraffin tissues, antigen retrieval with sodium citrate buffer (pH 6.0) is recommended.
Positive Control
Skeletal Muscle Lysate (NB820-59253)
Control Peptide
IDH2 Peptide (NBP2-22166PEP)
Publications
Read Publications using
NBP2-22166 in the following applications:

  • WB
    2 publications

Reactivity Notes

Human, mouse and rat. Predicted to react with monkey, hamster, Guinea pig and rabbit based on 100% sequence homology.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for IDH2 Antibody

  • D2HGA2
  • EC 1.1.1.42
  • ICD-M
  • IDH
  • IDHM
  • IDP
  • IDPM
  • isocitrate dehydrogenase [NADP], mitochondrial
  • isocitrate dehydrogenase 2 (NADP+), mitochondrial
  • mNADP-IDH
  • NADP(+)-specific ICDH
  • Oxalosuccinate decarboxylase

Background

Isocitrate dehydrogenase 2, also known as IDH, IDP, oxalosuccinate decarboxylase, IDPM, and mNADP-IDH, catalyzes the oxidative decarboxylation of isocitrate to 2-oxoglutarate. IDH2 is imperative for metabolic regulation and energy production, primarily via the citric acid cycle. In addition, IDH2 has been implicated in tight interactions with the pyruvate dehydrogenase complex. IDH2 is localized in the mitochondria and is found in stems, roots and leaves. Recent research has found that mutations in IDH2 can block histone demethylation and result in an inability for cells to differentiate in gliomas (PMID: 22343901). Further studies have also linked IDH2 to both gallbladder and bile duct cancer (PMID: 22180306).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IDH2 Antibody (NBP2-22166)(3)

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Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IDH2 Antibody (NBP2-22166) (0)

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Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

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Bioinformatics

Gene Symbol IDH2
Entrez
Uniprot