SDHB Antibody


Genetic Strategies: Western Blot: SDHB Antibody [NBP1-87069] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SDHB antibody. Remaining relative intensity more
Immunocytochemistry/ Immunofluorescence: SDHB Antibody [NBP1-87069] - Staining of human cell line PC-3 shows localization to nucleoplasm, plasma membrane & mitochondria. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Staining of human liver shows moderate to strong granular cytoplasmic postivity in hepatocytes.
Western Blot: SDHB Antibody [NBP1-87069] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Immunohistochemical staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Immunohistochemical staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.
Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Immunohistochemical staining of human duodenum shows moderate to strong granular cytoplasmic positivity in glandular cells.
Simple Western: SDHB Antibody [NBP1-87069] - Simple Western lane view shows a specific band for SDHB in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions more
Simple Western: SDHB Antibody [NBP1-87069] - Electropherogram image(s) of corresponding Simple Western lane view. SDHB antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).

Product Details

Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, KD
Validated by:

Genetic Strategies


Order Details

SDHB Antibody Summary

Mitochondria Marker
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:1000 - 1:2500
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
  • Knockdown Validated
  • Simple Western 1:20
  • Western Blot 0.04-0.4 ug/ml
Application Notes
IHC reported in literature (PMID:25720320). ICC/IF Fixation Permeabilization: Use PFA/Triton X-100. IHC-Paraffin HIER pH6 retrieval is recommended. In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
SDHB Protein (NBP1-87069PEP)
Read Publications using
NBP1-87069 in the following applications:

  • 6 publications
  • WB
    2 publications

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22170724)

Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS (pH 7.2) and 40% Glycerol
0.02% Sodium Azide
Immunogen affinity purified

Alternate Names for SDHB Antibody

  • EC
  • FLJ92337
  • IP
  • Iron-sulfur subunit of complex II
  • PGL4
  • SDH
  • SDH1
  • SDH2
  • succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
  • succinate dehydrogenase complex, subunit B, iron sulfur (Ip)


Complex II of the respiratory chain, which is specifically involved in the oxidation of succinate, carries electrons from FADH to CoQ. The complex is composed of four nuclear-encoded subunits and is localized in the mitochondrial inner membrane. The iron-sulfur subunit is highly conserved and contains three cysteine-rich clusters which may comprise the iron-sulfur centers of the enzyme. Sporadic and familial mutations in this gene result in paragangliomas and pheochromocytoma, and support a link between mitochondrial dysfunction and tumorigenesis. [provided by RefSeq]


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SDHB Antibody (NBP1-87069)(16)

We have publications tested in 2 confirmed species: Human, Rat.

We have publications tested in 2 applications: IF/IHC, WB.

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Showing Publications 1 - 10 of 16. Show All 16 Publications.
Publications using NBP1-87069 Applications Species
Kang K, Ko J, Lee H et al. Surgically Metabolic Resection of Pericardial Fat to Ameliorate Myocardial Mitochondrial Dysfunction in the Acute Myocardial Infarction Obese Rat Journal of Korean Medical Science 2022-02-08 [PMID: 35257523] (WB, Rat) WB Rat
Thomas G Papathomas, Lindsey Oudijk, Alexandre Persu et al. SDHB/SDHA immunohistochemistry in pheochromocytomas and paragangliomas: a multicenter interobserver variation analysis using virtual microscopy: a Multinational Study of the European Network for the Study of Adrenal Tumors (ENS@T). Modern Pathology 2015-02-27 [PMID: 25720320] (IF/IHC, Human) IF/IHC Human
Desmurs M, Foti M, Raemy E et al. C11orf83, a Mitochondrial Cardiolipin-Binding Protein Involved in bc1 Complex Assembly and Supercomplex Stabilization. Mol Cell Biol 2015-04-01 [PMID: 25605331] (WB, Human) WB Human
Bayley JP, Oldenburg RA, Nuk J et al. Paraganglioma and pheochromocytoma upon maternal transmission of SDHD mutations. BMC Med Genet 2014-10-10 [PMID: 25300370] (IF/IHC, Human) IF/IHC Human
Nannini M, Astolfi A, Urbini M et al. Integrated genomic study of quadruple-WT GIST (KIT/PDGFRA/SDH/RAS pathway wild-type GIST). BMC Cancer 2014-09-20 [PMID: 25239601] (IF/IHC, Human) IF/IHC Human
Clarissa A Cassol, Daniel Winer, Wei Liu et al. Tyrosine kinase receptors as molecular targets in pheochromocytomas and paragangliomas. Modern Pathology 2014-01-01 [PMID: 24390213] (IF/IHC, Human) IF/IHC Human
Jamilloux Y, Favier J, Pertuit M et al. A MEN1 syndrome with a paraganglioma. Eur J Hum Genet 2014-02-01 [PMID: 23778871] (IF/IHC, Human) IF/IHC Human
Pantaleo MA, Astolfi A, Urbini M et al. Analysis of all subunits, SDHA, SDHB, SDHC, SDHD, of the succinate dehydrogenase complex in KIT/PDGFRA wild-type GIST. Eur J Hum Genet 2014-01-01 [PMID: 23612575] (IF/IHC) IF/IHC
Rapizzi E, Ercolino T, Canu L et al. Mitochondrial function and content in pheochromocytoma/paraganglioma of succinate dehydrogenase mutation carriers. Endocr Relat Cancer 2012-05-01 [PMID: 22323561]
Xekouki P, Pacak K, Almeida M et al. Succinate Dehydrogenase (SDH) D Subunit (SDHD) Inactivation in a Growth-Hormone-Producing Pituitary Tumor: A New Association for SDH?. J Clin Endocrinol Metab 2012-03-01 [PMID: 22170724]
Show All 16 Publications.

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Product General Protocols

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ICC/IF Video Protocol

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Gene Symbol SDHB