SDHB Antibody

Images

 
Western Blot: SDHB Antibody [NBP1-87069] - Analysis in RT-4 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-SDHB antibody. Remaining relative intensity is presented. Loading ...read more
Immunocytochemistry/ Immunofluorescence: SDHB Antibody [NBP1-87069] - Immunofluorescent staining of human cell line PC-3 shows localization to nucleoplasm, plasma membrane & mitochondria.
Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Immunohistochemical staining of human duodenum shows moderate to strong granular cytoplasmic positivity in glandular cells.
Western Blot: SDHB Antibody [NBP1-87069] - Analysis in human cell line U-937 MG.
Western Blot: SDHB Antibody [NBP1-87069] - Analysis in mouse cell line NIH-3T3 and rat cell line NBT-II.
Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Staining of human liver shows strong cytoplasmic positivity in hepatocytes.
Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Immunohistochemical staining of human testis shows moderate to strong granular cytoplasmic positivity in cells in seminiferous ducts and Leydig cells.
Immunohistochemistry-Paraffin: SDHB Antibody [NBP1-87069] - Immunohistochemical staining of human kidney shows moderate to strong granular cytoplasmic positivity in cells in tubules.
Simple Western: SDHB Antibody [NBP1-87069] - Simple Western lane view shows a specific band for SDHB in 0.2 mg/ml of RT-4 (left) and U-251MG sp (right) lysate. This experiment was performed under reducing conditions ...read more
Simple Western: SDHB Antibody [NBP1-87069] - Electropherogram image(s) of corresponding Simple Western lane view. SDHB antibody was used at 1:20 dilution on RT-4 and U-251MG sp lysates(s).

Product Details

Summary
Reactivity Hu, Mu, RtSpecies Glossary
Applications WB, Simple Western, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated

Order Details

SDHB Antibody Summary

Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:EGKQQYLQSIEEREKLDGLYECILCACCSTSCPSYWWNGDKYLGPAVLMQAYRWMIDSRDDFTEERLAKLQDPFSLYRCHTIMNCTRTCPKGLNPGKAIAEIKKMMATY
Marker
Mitochondria Marker
Specificity
Specificity of antibody verified on a Protein Array containing target protein plus 383 other non-specific proteins.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
SDHB
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:100-1:500
  • Simple Western 1:20
  • Immunocytochemistry/Immunofluorescence 1-4 ug/ml
  • Immunohistochemistry
  • Immunohistochemistry-Paraffin 1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
Theoretical MW
28 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control Peptide
SDHB Protein (NBP1-87069PEP)
Publications
Read Publications using
NBP1-87069 in the following applications:

  • IHC
    6 publications
  • WB
    1 publication

Reactivity Notes

Reactivity reported in scientific literature (PMID: 22170724)

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Purity
Immunogen affinity purified

Alternate Names for SDHB Antibody

  • EC 1.3.5.1
  • FLJ92337
  • IP
  • Iron-sulfur subunit of complex II
  • PGL4
  • SDH
  • SDH1
  • SDH2
  • SDHIP
  • succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial
  • succinate dehydrogenase complex, subunit B, iron sulfur (Ip)

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for SDHB Antibody (NBP1-87069)(15)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: IHC, WB.


Filter By Application
IHC
(6)
WB
(1)
All Applications
Filter By Species
Human
(6)
All Species
Showing Publications 1 - 10 of 15. Show All 15 Publications.
Publications using NBP1-87069 Applications Species
Thomas G Papathomas, Lindsey Oudijk, Alexandre Persu et al. SDHB/SDHA immunohistochemistry in pheochromocytomas and paragangliomas: a multicenter interobserver variation analysis using virtual microscopy: a Multinational Study of the European Network for the Study of Adrenal Tumors (ENS@T). Modern Pathology 2015 Feb 27 [PMID: 25720320] (IHC, Human) IHC Human
Desmurs M, Foti M, Raemy E et al. C11orf83, a Mitochondrial Cardiolipin-Binding Protein Involved in bc1 Complex Assembly and Supercomplex Stabilization. Mol Cell Biol 2015 Apr [PMID: 25605331] (WB, Human) WB Human
Bayley JP, Oldenburg RA, Nuk J et al. Paraganglioma and pheochromocytoma upon maternal transmission of SDHD mutations. BMC Med Genet 2014 Oct 10 [PMID: 25300370] (IHC, Human) IHC Human
Nannini M, Astolfi A, Urbini M et al. Integrated genomic study of quadruple-WT GIST (KIT/PDGFRA/SDH/RAS pathway wild-type GIST). BMC Cancer 2014 Sep 20 [PMID: 25239601] (IHC, Human) IHC Human
Clarissa A Cassol, Daniel Winer, Wei Liu et al. Tyrosine kinase receptors as molecular targets in pheochromocytomas and paragangliomas. Modern Pathology 2014 [PMID: 24390213] (IHC, Human) IHC Human
Jamilloux Y, Favier J, Pertuit M et al. A MEN1 syndrome with a paraganglioma. Eur J Hum Genet 2014 Feb [PMID: 23778871] (IHC, Human) IHC Human
Pantaleo MA, Astolfi A, Urbini M et al. Analysis of all subunits, SDHA, SDHB, SDHC, SDHD, of the succinate dehydrogenase complex in KIT/PDGFRA wild-type GIST. Eur J Hum Genet 2014 Jan [PMID: 23612575] (IHC) IHC
Rapizzi E, Ercolino T, Canu L et al. Mitochondrial function and content in pheochromocytoma/paraganglioma of succinate dehydrogenase mutation carriers. Endocr Relat Cancer 2012 May [PMID: 22323561]
Xekouki P, Pacak K, Almeida M et al. Succinate Dehydrogenase (SDH) D Subunit (SDHD) Inactivation in a Growth-Hormone-Producing Pituitary Tumor: A New Association for SDH?. J Clin Endocrinol Metab 2012 Mar [PMID: 22170724]
Marchiani S, Tamburrino L, Giuliano L et al. Sumo1-ylation of human spermatozoa and its relationship with semen quality. Int J Androl 2011 Dec [PMID: 21039605]
Show All 15 Publications.

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Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for SDHB Antibody (NBP1-87069) (0)

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Secondary Antibodies

 

Isotype Controls

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Bioinformatics Tool for SDHB Antibody (NBP1-87069)

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Diseases for SDHB Antibody (NBP1-87069)

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Research Areas for SDHB Antibody (NBP1-87069)

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Blogs on SDHB.

HIF Antibodies: Beyond HIF-1 alpha
The hypoxia inducible factors are a family of heterodimeric transcription factors which are activated in response to lowered oxygen levels, or hypoxia. Although it may seem that HIF-1 alpha receives all the attention, other HIF antibodies, such as the...  Read full blog post.

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Bioinformatics

Gene Symbol SDHB