Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/6806R)

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/6806R) [NBP3-13943] - FFPE prostate carcinoma with IDH1-R132H mutation stained with Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1/6806R).

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/6806R) [NBP3-13943] - FFPE human glioblastoma with IDH1-R132H mutation stained with Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1/6806R). HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

Immunohistochemistry-Paraffin: Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/6806R) [NBP3-13943] - IHC analysis of formalin-fixed, paraffin-embedded human cerebellum. Negative tissue control using Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1/6806R) at 2ug/ml in PBS for 30min RT. HIER: Tris/EDTA, pH9.0, 45min. HRP-polymer, 30min. DAB, 5min.

FFPE prostate carcinoma with IDH1-R132H mutation stained with Isocitrate Dehydrogenase 1/IDH1 antibody (IDH1/6806R).

• Reactivity: Hu
• Applications: IHC
• Clone: IDH1/6806R
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Concentration: 0.2 mg/ml

Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/6806R) Summary

• Description: 200ug/ml of antibody purified from Bioreactor Concentrate by Protein A or G. Prepared in 1 mM PBS with 0.05% BSA & 0.05% azide. Also available WITHOUT BSA & azide at 1.0 mg/ml. (NBP3-14080)
  
  Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80 C. Non-hazardous.
  
  Theoretical molecular weight: 45-47kDa
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Recombinant fragment of human Isocitrate Dehydrogenase 1/IDH1 protein (around aa 281-414) (exact sequence is proprietary) (Uniprot: O75874 )
• Localization: Cytoplasm, Nucleus
• Specificity: IDH1 R132H antibody binds to IDH1-mutated protein, but does not bind the wild-type IDH1 protein. IDH1 R132H point mutations are frequently seen in World Health Organization grade II and III gliomas and are believed to constitute an early step in tumorigenesis. IDH1 R132H can be used as a diagnostic marker to help differentiate infiltrating gliomas from gliosis, and as a prognostic marker for gliomas and secondary glioblastoma multiforme. IDH1 R132H antibody shows strong cytoplasmic staining and weaker nuclear staining in tumor cells with the R132H-mutated peptide. Diffuse staining of the fibrillary tumor matrix is also seen.
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IDH1
• Purity: Protein A or G purified

Applications/Dilutions

• Dilutions:
  • Immunohistochemistry-Paraffin 1-2 ug/ml
• Application Notes: Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes

Packaging, Storage & Formulations

• Storage: Store at 4C.
• Buffer: 1mM PBS with 0.05% BSA
• Preservative: 0.05% Sodium Azide
• Concentration: 0.2 mg/ml
• Purity: Protein A or G purified

Alternate Names for Isocitrate Dehydrogenase 1/IDH1 Antibody (IDH1/6806R)

• Cytosolic NADP-isocitrate dehydrogenase
• EC 1.1.1.42
• IDCD
• IDH
• IDH1
• IDP
• IDPC
• isocitrate dehydrogenase [NADP] cytoplasmic
• isocitrate dehydrogenase 1 (NADP+), soluble
• Isocitrate Dehydrogenase 1
• NADP(+)-specific ICDH
• NADP-dependent isocitrate dehydrogenase, cytosolic
• NADP-dependent isocitrate dehydrogenase, peroxisomal
• Oxalosuccinate decarboxylase
• PICD

Background

Isocitrate dehydrogenases catalyze the oxidative decarboxylation of isocitrate to 2-oxoglutarate. These enzymes belong to two distinct subclasses, one of which utilizes NAD(+) as the electron acceptor and the other NADP(+). Five isocitrate dehydrogenases have been reported: three NAD(+)-dependent isocitrate dehydrogenases, which localize to the mitochondrial matrix, and two NADP(+)-dependent isocitrate dehydrogenases, one of which is mitochondrial and the other predominantly cytosolic. Each NADP(+)-dependent isozyme is a homodimer. The protein encoded by this gene is the NADP(+)-dependent isocitrate dehydrogenase found in the cytoplasm and peroxisomes. It contains the PTS-1 peroxisomal targeting signal sequence. The presence of this enzyme in peroxisomes suggests roles in the regeneration of NADPH for intraperoxisomal reductions, such as the conversion of 2, 4-dienoyl-CoAs to 3-enoyl-CoAs, as well as in peroxisomal reactions that consume 2-oxoglutarate, namely the alpha-hydroxylation of phytanic acid. The cytoplasmic enzyme serves a significant role in cytoplasmic NADPH production. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IDH1
• Uniprot:
  • O75874()
  • O75874()