IRAK4 Antibody (10H4) - BSA Free

Western Blot: IRAK4 Antibody (10H4) [NBP3-15153] - Positive WB detected in: THP-1 whole cell lysate, U251 whole cell lysate. All lanes: IRAK4 antibody at 1:2000. Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution. Predicted band size: 52, 38 KDa. Observed band size: 55 KDa.

Flow Cytometry: IRAK4 Antibody (10H4) [NBP3-15153] - Overlay histogram showing Jurkat cells stained with IRAK4 antibody (red line) at 1:50. The cells were fixed with 70% Ethylalcohol (18h) and then incubated in 10% normal goat serum to block non-specific protein-protein interactions followedby the antibody (1ug/1*106cells) for 1 h at 4C.The secondary antibody used was FITC-conjugated goat anti-rabbit IgG (H+L) at 1/200 dilution for 30min at 4C. Control antibody (green line) was Rabbit IgG (1ug/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.

• Reactivity: Hu
• Applications: WB, ELISA, Flow
• Clone: 10H4
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free

IRAK4 Antibody (10H4) - BSA Free Summary

• Description: Novus Biologicals Rabbit IRAK4 Antibody (10H4) - BSA Free (NBP3-15153) is a recombinant monoclonal antibody validated for use in WB, ELISA and Flow. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant monoclonal antibody expressed in HEK293F cells
• Immunogen: A synthesized peptide derived from human IRAK4. UniProt ID (Q9NWZ3).
• Source: HEK293
• Isotype: IgG
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IRAK4
• Purity: Affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA
  • Flow Cytometry
  • Western Blot 1:500-1:5000

Packaging, Storage & Formulations

• Storage: Store at -20 to -70C. Avoid freeze-thaw cycles.
• Buffer: PBS, pH 7.4, 150mM NaCl, and 50% glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Affinity purified

Alternate Names for IRAK4 Antibody (10H4) - BSA Free

• EC 2.7.11
• EC 2.7.11.1
• interleukin-1 receptor-associated kinase 4
• IPD1
• IRAK4
• IRAK-4
• NY-REN-64
• REN64
• Renal carcinoma antigen NY-REN-64

Background

Interleukin-1 receptor (see IL1R; MIM 147810)-associated kinases (e.g., IRAK1; MIM 300283) are important mediators in the signal transduction of Toll-like receptor (TLR, e.g., TLR4; MIM 603030) and IL1R family members, collectively referred to as TIRs. IRAK4 functions in this signal transduction pathway.[supplied by OMIM]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRAK4