IRAK3 Antibody - BSA Free

Western Blot: IRAK3 Antibody [NBP1-76782] - Analysis of IRAK3 in mouse spleen (M) and rat liver (R) tissue lysates with IRAK3 antibody using this antibody at 1 ug/ml.

Immunohistochemistry-Paraffin: IRAK3 Antibody [NBP1-76782] - Staining of human spleen tissue with antibody at 5 ug/mL.

Immunocytochemistry/ Immunofluorescence: IRAK3 Antibody - BSA Free [NBP1-76782] - Immunofluorescence of IRAK2 in A20 cells with IRAK2 antibody at 20 ug/mL.Red: IRAK3 Antibody Blue: DAPI staining

Immunohistochemistry: IRAK3 Antibody [NBP1-76782] - Rat Liver tissue with IRAK-M antibody at 10 ug/mL.

Immunohistochemistry: IRAK3 Antibody - BSA Free [NBP1-76782] - Immunohistochemical staining of rat liver tissue using IRAK3 antibody at 2 ug/mL.

• Reactivity: Hu, Mu, Rt
• Applications: WB, ELISA, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: BSA Free
• Concentration: 1 mg/ml

IRAK3 Antibody - BSA Free Summary

• Description: Novus Biologicals Rabbit IRAK3 Antibody - BSA Free (NBP1-76782) is a polyclonal antibody validated for use in IHC, WB, ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IRAK-M. The immunogen is located within the last 50 amino acids of IRAK-M. Amino Acid Squence: CSSKFSWDEYEQYKKE
• Specificity: IRAK-M antibody is predicted to have no cross reactivity to other members of the IRAK family.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IRAK3
• Purity: Peptide affinity purified

Applications/Dilutions

• Dilutions:
  • ELISA 1:100-1:2000
  • Immunocytochemistry/ Immunofluorescence 10 ug/mL
  • Immunohistochemistry 2 ug/ml
  • Immunohistochemistry-Paraffin 2 ug/ml
  • Western Blot 1 ug/ml
• Theoretical MW: 70 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
• Control Peptide:

  IRAK3 Peptide (NBP1-76782PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS
• Preservative: 0.02% Sodium Azide
• Concentration: 1 mg/ml
• Purity: Peptide affinity purified

Alternate Names for IRAK3 Antibody - BSA Free

• ASRT5
• EC 2.7.11.1
• FLJ13601
• IL-1 receptor-associated kinase M
• interleukin-1 receptor-associated kinase 3
• IRAK3
• IRAK-3
• IRAKM
• IRAK-M

Background

Interleukin-1 (IL-1) and lipopolysaccharide (LPS) induces cellular response through IL-1 receptor (IL-1R) and Toll like receptors (TLR). IL-1 receptor-associated kinase (IRAK and IRAK2) mediates the activation of NF-betaB by IL-1/Toll receptors, which is a pivotal transcription factor mediating inflammatory and immune response. A novel member in the IRAK/Pelle family was recently identified and designated IRAK-M. IRAKs associate with IL-1/Toll receptors after IL-1 or LPS stimulation and the dominant negative mutants of IRAKs inhibit IL-1 or LPS induced NF-betaB activation. Members in IRAK/Pelle family play a central role in IL-1R/TLR mediated inflammatory responses to cytokine IL-1 and LPS.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

FAQs for IRAK3 Antibody (NBP1-76782). (Showing 1 - 1 of 1 FAQ).

1. Could you tell me the exact antigen or imunogen for IRAk3 antibody NBP1-76782?
   • Unfortunately, we cannot share the exact 16 amino acids of the peptide that the antibody was raised against because that info is proprietary. However, we can confirm you that the immunogen is located within the last 50 amino acids of IRAK-M protein ( C terminal or last 50 amino acids: P SQDLRPYKVN IDPSSEAPGH SCRSRPVESS CSSKFSWDEY EQYKKE; Uniprot ID Q9Y616). 

Bioinformatics

• Gene Symbol: IRAK3
• Uniprot:
  • AF113136(Human)