IRAK3 Antibody

Western Blot: IRAK3 Antibody [NB100-96912] - Staining (0.1ug/ml) of Rat Liver lysate (35ug protein in RIPA buffer). Detected with chemiluminescence.

Western Blot: IRAK3 Antibody [NB100-96912] - (0.5ug/ml) staining of Mouse Bone Marrow (wildtype first lane, KO second lane) lysates (35ug protein in RIPA buffer). Primary incubation was overnight at 4C Detected with chemiluminescence. Data obtained from anonymous customer.

• Reactivity: Mu, Rt
• Applications: WB, ELISA
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Unconjugated
• Concentration: 0.5 mg/ml

IRAK3 Antibody Summary

• Description: Novus Biologicals Goat IRAK3 Antibody (NB100-96912) is a polyclonal antibody validated for use in WB and ELISA. Anti-IRAK3 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: Peptide with sequence C-GHSYGSKPMEKR corresponding to internal region (near C-Terminus) according to NP_082955.2.
• Epitope: GHSYGSKPMEKR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IRAK3
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Peptide ELISA Detection limit 1:64000
  • Western Blot 0.03 - 0.1 ug/ml
• Application Notes: WB: Approx 65-70 kDa band observed in Rat Liver lysates, and in preliminary testing of Rat Adrenal Gland lysate (calculated MW of 69.0 kDa according to NP_001101571.1 and 67.8 kDa according to NP_082955.2). An approx. 70 kDa band is observed in mouse bone marrow lysates but this band disappears in lysates from the knock-out mouse.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
• Preservative: 0.02% Sodium Azide
• Concentration: 0.5 mg/ml
• Purity: Immunogen affinity purified

Alternate Names for IRAK3 Antibody

• ASRT5
• EC 2.7.11.1
• FLJ13601
• IL-1 receptor-associated kinase M
• interleukin-1 receptor-associated kinase 3
• IRAK3
• IRAK-3
• IRAKM
• IRAK-M

Background

reduced expression of IRAK-1 and increased expression of IRAK-M after CpG DNA pretreatment resulted in the hyporesponsiveness of macrophages that leads to the protection of mice from hepatic injury and death caused by CpG DNA/D-GalN. Endotoxin tolerance was significantly reduced in macrophages from IRAK-M knockout mice. IRAK-M regulates TLR signaling and innate immune homeostasis. induction of IRAK-M and inhibition of kinase activity of IRAK-1 are crucial to PGN-induced tolerance in macrophages. IRAK-M is a key regulator of the bone loss that is due to osteoclastic resorption of bone. IRAK-M induction negatively regulates Toll-like receptor-dependent interleukin-12 p40 production in macrophages in the presence of Mycobacterium tuberculosis lipoarabinomannan. These findings indicate that IRAK-M selectively attenuates p38 activation and inhibits innate immunity through stabilizing MKP-1. IRAK-M is directly involved in the regulation of both innate and adaptive immune signaling processes, and deletion of IRAK-M enhances host anti-tumor immune response.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IRAK3 Antibody (NB100-96912)(1)

Publication using NB100-96912

• Balaci L, Spada MC, Olla N et al. IRAK-M is involved in the pathogenesis of early-onset persistent asthma. Am J Hum Genet 2007-06-01 [PMID: 17503328]

Bioinformatics

• Gene Symbol: IRAK3
• Entrez:
  • 73914(Mouse)
  • 314870(Rat)
• Uniprot:
  • NP_082955.2(Mouse)