SOCS-1 Antibody

Images

 
Western Blot: SOCS-1 Antibody [NB100-781] - (mock transfection in lane B).
Immunohistochemistry-Paraffin: SOCS-1 Antibody [NB100-781] - (2.5ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. This data is from a previous batch.
Western Blot: SOCS-1 Antibody [NB100-781] - Staining (2ug/ml) of mouse spleen lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Product Details

Summary
Reactivity Hu, MuSpecies Glossary
Applications WB, IHC, IHC-P, PEP-ELISA
Clonality
Polyclonal
Host
Goat
Conjugate
Unconjugated
Concentration
0.5 mg/ml

Order Details

SOCS-1 Antibody Summary

Immunogen
Peptide with sequence C-VLRDYLSSFPFQI corresponding to C-Terminus according to NP_003736.1.
Isotype
IgG
Clonality
Polyclonal
Host
Goat
Gene
SOCS1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1-3 ug/ml
  • Immunohistochemistry 2 - 3 ug/ml
  • Immunohistochemistry-Paraffin 2-3 ug/ml
  • Peptide ELISA detection limit 1:32000
Application Notes
WB: In transfected HEK293 transiently expressing human SOCS1 bands of approx. 30 kDa band and 25 kDa band are observed. These bands are not observed in the non-transfected HEK293. A similar pattern is observed in mouse spleen lysates. The calculated molecular size is 23.6 kDa band according to human NP_003736.1 and 23.7 kDa band according to mouse NP_034026.1.
IHC-P: Human kidney shows strong staining of the Brush Border in PCT.
Positive Control
Spleen Lysate (NB820-59670)
SOCS-1 Lysate (NBL1-16325)
Publications
Read Publication using NB100-781.

Reactivity Notes

Predicted cross-reactivity based on sequence identity: Rat, Canine, Bovine.

Packaging, Storage & Formulations

Storage
Store at -20C. Avoid freeze-thaw cycles.
Buffer
0.5 mg/ml Tris (pH 7.3) and 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Purity
Immunogen affinity purified

Alternate Names for SOCS-1 Antibody

  • CIS1
  • Cish1
  • JAB
  • JABTec-interacting protein 3
  • JAK binding protein
  • JAK-binding protein
  • SOCS1
  • SOCS-1
  • SOCS-1CIS1
  • SSI-1
  • SSI-1CISH1
  • SSI1cytokine-inducible SH2 protein 1
  • STAT induced SH3 protein 1
  • STAT-induced STAT inhibitor 1
  • suppressor of cytokine signaling 1
  • TIP3
  • TIP-3
  • TIP3Cish1

Background

Cytokines induce a variety of biologic responses by binding to specific cell surface receptors and activating cytoplasmic signal transduction pathways. Proteins that mediate these signals from the cell surface to the nucleus include Janus kinases, e.g., Janus kinase-1, and the signal transducers and activators of transcription (STATs), such as STAT1. To maintain tight control of these signal transduction pathways, mechanisms for turning off the signals are necessary. The adverse consequences of an imbalance between positive and negative signals is evident in the 'motheaten' mouse, which lacks the phosphatase SHP1. These mice fail to regulate cytokine responses appropriately, resulting in hyperproliferation and accumulation of cells in several hematopoietic lineages and the development of systemic autoimmune disease. SOCS1 represents a family of proteins that regulates responses to cytokines. SOCS1 (also known as Janus kinase-binding protein and STAT-induced STAT inhibitor-1) was isolated independently by its ability to interact with Janus kinase-2 in a 2-hybrid screen and on the basis of antigenic similarity with the SH2 domain of STAT3. The SOCS family comprises at least 8 members, SOCS1 to SOCS7 and CIS (cytokine-inducible SH2-containing protein).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NBP1-30943
Species: Hu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-27210
Species: Hu, Fe, Pm
Applications: WB, IHC-P
AF2988
Species: Mu, Rt
Applications: WB
AF2894
Species: Hu, Mu
Applications: WB, Simple Western, CyTOF-ready, ICFlow
NB400-141
Species: Hu, Mu, Ha
Applications: WB, ICC/IF, IP
NBP2-22471
Species: Hu, Mu, Rt, Mk, Pm
Applications: WB, ChIP, ICC/IF, IHC, IHC-P, IP
AF4979
Species: Hu
Applications: WB
MAB4260
Species: Hu, Mu, Rt
Applications: WB, IHC, CyTOF-ready, ICC, ICFlow
AF1584
Species: Hu, Mu
Applications: WB, Simple Western, IP, CyTOF-ready, ICC, ICFlow
NBP2-00622
Species: Hu, Rt, Ca, Pm
Applications: WB, Flow, ICC/IF, IHC, IHC-P
NB200-106
Species: Mu
Applications: WB, ELISA, ICC/IF, IHC, IHC-Fr, IHC-P
NB100-56566
Species: Hu, Mu, Rt
Applications: WB, ChIP, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, In vitro, CyTOF-ready, Flow-CS, Flow-IC
NBP2-25241
Species: Hu, Mu
Applications: WB, ICC/IF, IP

Publications for SOCS-1 Antibody (NB100-781)(1)


Reviews for SOCS-1 Antibody (NB100-781) (0)

There are no reviews for SOCS-1 Antibody (NB100-781). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen
  • 1st to review with an image -- $50/€35/£30/$50 CAD/¥300 Yuan/¥5000 Yen

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for SOCS-1 Antibody (NB100-781) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Positive Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Additional SOCS-1 Products

Bioinformatics Tool for SOCS-1 Antibody (NB100-781)

Discover related pathways, diseases and genes to SOCS-1 Antibody (NB100-781). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for SOCS-1 Antibody (NB100-781)

Discover more about diseases related to SOCS-1 Antibody (NB100-781).
 

Pathways for SOCS-1 Antibody (NB100-781)

View related products by pathway.

PTMs for SOCS-1 Antibody (NB100-781)

Learn more about PTMs related to SOCS-1 Antibody (NB100-781).
 

Research Areas for SOCS-1 Antibody (NB100-781)

Find related products by research area.

Blogs on SOCS-1

There are no specific blogs for SOCS-1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Review this Product

Be the first to review our SOCS-1 Antibody and receive a gift card or discount.

Bioinformatics

Gene Symbol SOCS1
Entrez
Uniprot