IRAK3 Antibody [Unconjugated]

Western blot shows lysates of EOL-1 human acute myeloid (eosinophilic) leukemia cell line and THP-1 human acute monocytic leukemia cell line. PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). A specific band was detected for IRAK3 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Western blot shows recombinant human IRAK1, recombinant human IRAK2, recombinant human IRAK3, and recombinant human IRAK4 (2 ng/lane). PVDF Membrane was probed with 1 µg/mL of Sheep Anti-Human IRAK3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6264) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (HAF016). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1

IRAK3 isoform expression in primed macrophages. IRAK3 N-terminal to death domain (DD) (a.a. 1–14) is shaded grey, DD (15–104) is shaded blue, and pseudokinase domain (a.a. 171–446) is shaded green. (A) “Classical” full-length (CLS; NP_009130.2 a.a. 1–596). (B) “Alternative” IRAK-3 (ALT; NP_009130.2 a.a. 1–44, 105–596 of CLS) that lacks most of the DD. (C) Western blotting for IRAK3 in HEK293 cells (NIL) or HEK293 cells overexpressing classical or alternative IRAK3 blotted with two antibodies for IRAK3. (D) IRAK3 expression in monocyte-derived Mφ, unstimulated (NIL) or stimulated for 20 h with LPS, LPS/IFN gamma (M1), TNF, GM-CSF (GM), IL-4, or TGF-beta 1 (TGF); representative blots of two donors stained for IRAK3 (SAB3500193) and beta -actin (AC-74). Gene expression of (E) both IRAK3 isoforms and (F) the classical IRAK3 isoform in TNF-stimulated human Mφ. (G) Gene expression of Irak3 in LPS-stimulated (10 ng/mL) murine bone marrow-derived Mφ from wild-type or transgenic mice lacking either Tnfrsf1a or Tnfrsf1b; ANOVA for each respective knockout (KO) vs. wild type (WT), † p TM.

• Applications: WB, Simple Western
• Clonality: Polyclonal
• Host: Sheep
• Conjugate: Unconjugated
• Concentration: LYOPH

IRAK3 Antibody [Unconjugated] Summary

• Immunogen: E. coli-derived recombinant human IRAK3
  aa 1-596
  Accession # Q9Y616
• Specificity: Detects human IRAK3 in Western blots.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Sheep
• Gene: IRAK3
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • Simple Western 20 ug/mL
  • Western Blot 1 ug/mL

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IRAK3 Antibody [Unconjugated]

• ASRT5
• EC 2.7.11.1
• FLJ13601
• IL-1 receptor-associated kinase M
• interleukin-1 receptor-associated kinase 3
• IRAK3
• IRAK-3
• IRAKM
• IRAK-M

Background

IRAK3 (Interleukin-1 receptor-associated kinase 3; also IRAK-M) is a 68‑70 kDa member of the Pelle subfamily, TKL Ser/Thr protein kinase family of proteins. IRAK3 has limited expression, being mainly found in macrophages, eosinophils and respiratory epithelium, including type II alveolar cells. IRAK3 is a negative regulator of TLR signaling. Following TLR activation, it appears to form a complex with MyD88, IRAK1 and IRAK4 on the TLR cytoplasmic domain. This effectively blocks IRAK1/4 phosphorylation and inhibits NF kappa B and MAPK downstream activation. Human IRAK3 is 596 amino acids (aa) in length, contains one death domain (aa 41‑106) and a nonfunctional protein kinase domain (aa 171‑443). There are six Ser phosphorylation sites. There is one splice variant with a deletion of aa 45‑105.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IRAK3
• Uniprot:
  • Q9Y616(Human)