Integrin alpha 8 Antibody (481709) [Phycoerythrin]

H4 human neuroglioma cell line was stained with Mouse Anti-Human Integrin a8 PE-conjugated Monoclonal Antibody (Catalog # FAB6194P, filled histogram) or isotype control antibody (Catalog # IC0041P, open histogram). View our protocol for Staining Membrane-associated Proteins.

• Reactivity: Hu
• Applications: Flow
• Clone: 481709
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Phycoerythrin

Integrin alpha 8 Antibody (481709) [Phycoerythrin] Summary

• Immunogen: E. coli-derived recombinant human Integrin alpha 8
  Glu610-Leu800
  Accession # P53708
• Specificity: Detects human Integrin alpha 8 in direct ELISAs and Western blots.
• Source: N/A
• Isotype: IgG2b
• Clonality: Monoclonal
• Host: Mouse
• Gene: ITGA8
• Purity Statement: Protein A or G purified from hybridoma culture supernatant

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 10 uL/10^6 cells

Packaging, Storage & Formulations

• Storage: Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.
• Buffer: Supplied in a saline solution containing BSA and Sodium Azide.
• Preservative: Sodium Azide

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Integrin alpha 8 Antibody (481709) [Phycoerythrin]

• Integrin alpha 8
• integrin alpha-8
• integrin, alpha 8
• ITGA8

Background

Integrin alpha 8 is a 170-200 kDa member of the Integrin family of adhesion molecules. It forms an exclusive noncovalent heterodimer with Integrin beta 1. Integrin alpha 8/ beta 1 promotes both cell adhesion and survival, and is known to bind to fibronectin, LAP-TGF beta 1 and Nephronectin. It is principally expressed in smooth muscle cells.  Human alpha 8 is a 1025 amino acid (aa) type I transmembrane glycoprotein. It contains a 974 aa extracellular domain (ECD) (aa 39-1012) and a 30 aa cytoplasmic domain. In the ECD, human alpha 8 shares 90% aa sequence identity with mouse alpha 8 protein.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for Integrin alpha 8 Antibody (FAB6194P)(1)

Publication using FAB6194P

• Jurets A, Le Bras M, Staffler G, Stein G, Leitner L, Neuhofer A, Tardelli M, Turkof E, Zeyda M, Stulnig T Inhibition of Cellular Adhesion by Immunological Targeting of Osteopontin Neoepitopes Generated through Matrix Metalloproteinase and Thrombin Cleavage. PLoS ONE, 2016-02-03;11(2):e0148333. 2016-02-03 [PMID: 26840958] (Flow Cytometry, Human)

Bioinformatics

• Gene Symbol: ITGA8
• Uniprot:
  • P53708()