Integrin alpha 4/CD49d Antibody (21.6) - Chimeric - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: Integrin alpha 4/CD49d Antibody (21.6) [NBP2-81092] - Immunofluorescence staining of fixed K562 cells with Integrin alpha 4/CD49d Antibody (21.6) [NBP2-81092]. Immunofluorescence analysis of paraformaldehyde fixed K562 cells on Shi-fix coverslips, permeabilized with 0.15% Triton and stained with the chimeric rabbit IgG version of 21.6 [NBP2-81092] at 10 ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (1 ug/ml), showing memrbane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom NBP2-81092, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor 488 secondary antibody.

• Reactivity: Hu
• Applications: ELISA, ICC/IF, B/N
• Clone: 21.6
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

Integrin alpha 4/CD49d Antibody (21.6) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit Integrin alpha 4/CD49d Antibody (21.6) - Chimeric - Azide and BSA Free (NBP2-81092) is a recombinant monoclonal antibody validated for use in ELISA and ICC/IF. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody
• Immunogen: Integrin alpha 4/CD49d
• Specificity: This antibody binds to human Integrin alpha 4/CD49d
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: ITGA4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • Block/Neutralize
  • ELISA
  • Immunocytochemistry/ Immunofluorescence
• Application Notes: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG format, for improved compatibility with existing reagents, assays and techniques. This antibody binds to human alpha-4-integrin and can inhibit the binding of lymphocytes to endothelial cells, suppressing inflammation.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for Integrin alpha 4/CD49d Antibody (21.6) - Chimeric - Azide and BSA Free

• 269C wild type
• CD49 antigen-like family member D
• CD49d antigen
• CD49d
• CD49Dantigen CD49D, alpha-4 subunit of VLA-4 receptor
• IA4
• Integrin alpha 4
• integrin alpha-4 subunit
• integrin alpha-4
• Integrin alpha-IV
• integrin, alpha 4 (antigen CD49D, alpha 4 subunit of VLA-4 receptor)
• ITGA4
• MGC90518
• very late activation protein 4 receptor, alpha 4 subunit
• VLA-4 subunit alpha
• VLA-4

Background

VLA-4 is a cell surface heterodimer in the integrin superfamily of adhesion receptors. Anti-VLA-4 antibodies inhibited cytolytic T cell activity, with inhibitory activity directed against the effector T cells rather than their targets. Thus, whereas other VLA receptors appear to mediate cell--matrix interactions, VLA-4 may have a cell--cell adhesion function. Alpha 4 stands apart from all other known integrin alpha subunit sequences because (i) alpha 4 has neither an inserted I-domain, nor a disulfide-linked C-terminal fragment, (ii) its sequence is the most unique and (iii) only alpha 4 has a potential protease cleavage site, near the middle of the coding region, which appears responsible for the characteristic 80,000 and 70,000 Mr fragments of alpha 4 (1). Unlike any other integrin alpha subunit, the intact (150 kDa) alpha 4 subunit of VLA-4 can sometimes be cleaved into two noncovalently associated fragments (80 and 70 kDa) (2). The VLA-4 integrins are indispensable for embryogenesis, haematopoiesis and immune responses, possibly because alpha4 regulates cellular functions differently from other integrins through its cytoplasmic tail (3).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: ITGA4