Recombinant Human Insulin Protein

Product Discontinued

Recombinant Human Insulin Protein Summary

• Description: Recombinant biologically active INS protein.

  Source:E. coli.

• Details of Functionality: The Biological Activity was determined to be 28 units/mg.
• Source: E. coli
• Protein/Peptide Type: Recombinant Protein
• Gene: INS
• Purity: >98%, by HPLC.
• Endotoxin Note: Less than 0.1 ng/ug (IEU/ug) of recombinant Insulin.

Applications/Dilutions

• Dilutions:
  • Bioactivity
• Theoretical MW: 5.8 kDa.
  Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Lyophilized from a concentrated solution with no additives.
• Preservative: No Preservative
• Concentration: LYOPH
• Purity: >98%, by HPLC.
• Reconstitution Instructions: Reconstitute the lyophilized Insulin in sterile 0.005N HCl not more than 1 mg/ml.

Notes

After reconstitution, store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Alternate Names for Recombinant Human Insulin Protein

• IDDM2
• ILPR
• INS
• Insulin
• IRDN
• MODY10
• proinsulin

Background

Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 3 months from date of receipt.

Publications for Insulin Recombinant Protein (NBP2-26530)(2)

Publications using NBP2-26530

• Parida PK, Marquez-Palencia M, Nair V et al. Metabolic diversity within breast cancer brain-tropic cells determines metastatic fitness Cell metabolism 2022-01-04 [PMID: 34986341]
• Hashim M, Korupolu R, Syed B et al. Jejunal wall delivery of insulin via an ingestible capsule in anesthetized swine-A pharmacokinetic and pharmacodynamic study Pharmacol Res Perspect 2019-10-01 [PMID: 31584244]

FAQs for Insulin Recombinant Protein (NBP2-26530). (Showing 1 - 1 of 1 FAQs).

1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
   • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

Bioinformatics

• Gene Symbol: INS