Recombinant Human Insulin Protein

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SDS-Page: Recombinant Human Insulin Protein [NBP1-99193] - 2, 5 and 10 ug of human recombinant insulin loaded in each lane under reducing conditions and stained with Comasie blue. Human recombinant insulin has predicted ...read more
SDS-Page: Recombinant Human Insulin Protein [NBP1-99193]

Product Details

Summary
Reactivity HuSpecies Glossary
Applications PAGE
Concentration
Lyoph

Order Details

Recombinant Human Insulin Protein Summary

Description
Recombinant bioactive protein containing 51 amino acids for Human Insulin

Source:E. coli
Amino Acid Sequence:(P01308)

Preparation
Method
Determined to be >98% pure by SDS-PAGE
Details of Functionality
The recombinant insulin is fully biologically active when compared to World Health Organization (WHO) reference standard which is 28 units/mg.
Source
E. coli
Protein/Peptide Type
Biologically Active Protein
Gene
INS
Purity
>95%, by SDS-PAGE
Endotoxin Note
<0.1 ng/ug

Applications/Dilutions

Theoretical MW
5.81 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Packaging, Storage & Formulations

Storage
Store at -20 to -80C. Avoid freeze-thaw cycles.
Buffer
Lyophilized powder
Concentration
Lyoph
Purity
>95%, by SDS-PAGE
Reconstitution Instructions
Reconstitute in H20 to a concentration of 1.0 mg/ml. The solution can then be diluted to other aqueous buffers.

Alternate Names for Recombinant Human Insulin Protein

  • IDDM2
  • ILPR
  • INS
  • Insulin
  • IRDN
  • MODY10
  • proinsulin

Background

Recombinant Human insulin produced in E.Coli is a two chain, non-glycosylated polypeptide chain containing 51 amino acids and having a molecular mass of 5.81 kDa. Recombinant insulin is purified by proprietary chromatographic techniques.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Peptides and proteins are guaranteed for 2 years from date of receipt.

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FAQs for Insulin Recombinant Protein (NBP1-99193). (Showing 1 - 1 of 1 FAQ).

  1. Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.
    • I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

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Bioinformatics

Gene Symbol INS
Entrez