IL-6 Overexpression Lysate (Native) Summary
The lysate was created in HEK293T cells, using plasmid ID RC202078 and based on accession number NM_000600. The protein contains a C-terminal DDK tag.
Homo sapiens interleukin 6 (interferon, beta 2) (IL6), mRNA.
Packaging, Storage & Formulations
Store at -80C. Avoid freeze-thaw cycles.
The exact concentration of the protein of interest cannot be determined for overexpression lysates. Please contact technical support for more information.
This product is intended for use as a positive control in Western Blot. You will receive the lysate (100ug), and an empty vector negative control (100 ug).
Lysate Details for IL-6
HEK293T cells in 10-cm dishes were transiently transfected with a non-lipid polymer transfection reagent specially designed and manufactured for large volume DNA transfection. Transfected cells were cultured for 48hrs before collection. The cells were lysed in modified RIPA buffer (25mM Tris-HCl pH7.6, 150mM NaCl, 1% NP-40, 1mM EDTA, 1xProteinase inhibitor cocktail mix, 1mM PMSF and 1mM Na3VO4, and then centrifuged to clarify the lysate. Protein concentration was measured by BCA protein assay kit.This product is manufactured by and sold under license from OriGene Technologies and its use is limited solely for research purposes.
Alternate Names for IL-6 Overexpression Lysate (Native)
- B cell stimulatory factor-2
- B-cell differentiation factor
- BSF2CTL differentiation factor
- HGFHSFIFNB2Hybridoma growth factor
- IFN-beta 2
- IL-6B-cell stimulatory factor 2
- Interferon beta-2
- interleukin 6 (interferon, beta 2)
- interleukin BSF-2
This gene encodes a cytokine that functions in inflammation and the maturation of B cells. The protein is primarily produced at sites of acute and chronic inflammation, where it is secreted into the serum and induces a transcriptional inflammatory response through interleukin 6 receptor, alpha. The functioning of this gene is implicated in a wide variety of inflammation-associated disease states, including suspectibility to diabetes mellitus and systemic juvenile rheumatoid arthritis. [provided by RefSeq]. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Lysates are guaranteed
for 6 months from date of receipt.
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Product General Protocols
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FAQs for IL-6 Lysate (NBL1-11962). (Showing 1 - 1 of 1 FAQ).
I was wondering if you know of any blocking antibodies against IL-6 or VEGF-C that can be used in cell culture to quench these cytokines?
- We have no antibodies that are currently known to show blocking antibodies to VEGF-C. Please see this link to our IL-6 antibodies with blocking/neutralizing activity can be found at this link.
Additional IL-6 Overexpression Lysate (Native) Products
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Bioinformatics Tool for IL-6 Lysate (NBL1-11962)
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