IL-4 Antibody (11B11) - Chimeric - Azide and BSA Free

• Reactivity: Mu
• Applications: ELISA, Flow, Func, IHC, CyTOF-ready
• Clone: 11B11
• Clonality: Monoclonal
• Host: Rabbit
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 1 mg/ml

IL-4 Antibody (11B11) - Chimeric - Azide and BSA Free Summary

• Description: Novus Biologicals Rabbit IL-4 Antibody (11B11) - Chimeric - Azide and BSA Free (NBP2-52656) is a recombinant monoclonal antibody validated for use in IHC, ELISA and Flow. Anti-IL-4 Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Additional Information: Recombinant Monoclonal Antibody.
• Immunogen: BSF-1 / IL-4 purified from serum-free supernatants of cultured EL-4 cells (a C57BL/6 thymoma) induced with 4 Beta-phorbol 12 Beta-myristate 12 alpha -acetate for 48h.
• Specificity: Binds to a core functional region of murine IL-4, which prevents this cytokine from interacting with its cognate receptor (Yao et al., 2006 PMID: 16361318).
• Isotype: IgG Kappa
• Clonality: Monoclonal
• Host: Rabbit
• Gene: IL4
• Purity: Protein A purified

Applications/Dilutions

• Dilutions:
  • CyTOF-ready
  • ELISA 1:100 - 1:2000
  • Flow Cytometry 1:10 - 1:1000
  • Immunohistochemistry 1:10 - 1:500
  • In vivo assay
• Application Notes: This antibody is Cytof ready. This chimeric rabbit antibody was made using the variable domain sequences of the original Rat IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Packaging, Storage & Formulations

• Storage: Store at 4C for up to 3 months. For longer storage, aliquot and store at -20C.
• Buffer: PBS
• Preservative: 0.02% Proclin 300
• Concentration: 1 mg/ml
• Purity: Protein A purified

Alternate Names for IL-4 Antibody (11B11) - Chimeric - Azide and BSA Free

• B cell growth factor 1
• BCDF
• B-cell stimulatory factor 1
• BCGF1
• BCGF-1
• binetrakin
• BSF1
• BSF-1
• IL4
• IL-4
• IL-4B_cell stimulatory factor 1
• IL4E12
• interleukin 4
• interleukin-4
• Lymphocyte stimulatory factor 1
• MGC79402
• pitrakinra

Background

IL-4 (Interleukin 4, B cell stimulatory factor) is a pleiotropic cytokine produced by activated T cells, mast cells and basophils. IL-4 has many different functions including promoting proliferation and differentiation of immunologically competent cells. One of its main functions is regulating differentiation of naive CD4+ T cells into helper Th2 cells, the other main function is regulating B cells IgE and IgG1 production. Human IL-4 shows 50% homology with mouse IL-4, but its activities are species-specific and human IL-4 shows no activity on murine and rat cells.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-4 Antibody (NBP2-52656)(1)

Publication using NBP2-52656

• Li H, Wang Y, Shao S et al. Rabdosia serra alleviates dextran sulfate sodium salt-induced colitis in mice through antiinflammation, regulating Th17/Treg balance, maintaining intestinal barrier integrity, and modulating gut microbiome Journal of Pharmaceutical Analysis 2022-08-01 [PMID: 36605573]

Bioinformatics

• Gene Symbol: IL4