IL-4 Antibodies

404-ML) stimulates proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat Anti-Mouse IL-4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-4 Monoclonal Antibody (Catalog # MAB404). The ND₅₀ is typically 0.1-0.6 µg/mL." class="big_lightbox"> 404-ML) stimulates proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat Anti-Mouse IL-4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-4 Monoclonal Antibody (Catalog # MAB404). The ND₅₀ is typically 0.1-0.6 µg/mL." alt="Recombinant Mouse IL-4 (Catalog # 404-ML) stimulates proliferation in the HT-2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat Anti-Mouse IL-4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-4 Monoclonal Antibody (Catalog # MAB404). The ND₅₀ is typically 0.1-0.6 µg/mL." class="big_thumb" />

404-ML) was serially diluted and captured by Rat Anti-Mouse IL‑4 Monoclonal Antibody (Catalog # MAB404) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-404-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 404-ML) was serially diluted and captured by Rat Anti-Mouse IL‑4 Monoclonal Antibody (Catalog # MAB404) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-404-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB204). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_lightbox"> 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB204). The ND₅₀ is typically 0.5-1.5 µg/mL." alt="Recombinant Human IL-4 (Catalog # 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB204). The ND₅₀ is typically 0.5-1.5 µg/mL." class="big_thumb" />

204-IL) was serially diluted and captured by Mouse Anti-Human IL‑4 Monoclonal Antibody (Catalog # MAB604) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-204-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 204-IL) was serially diluted and captured by Mouse Anti-Human IL‑4 Monoclonal Antibody (Catalog # MAB604) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-204-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human IL‑4 (Catalog # 204-IL) was serially diluted and captured by Mouse Anti-Human IL‑4 Monoclonal Antibody (Catalog # MAB604) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-204-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-4 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) stimulated with PHA using Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB304) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB304). The ND₅₀ is typically 0.03-0.1 µg/mL." class="big_lightbox"> 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human IL-4 Monoclonal Antibody (Catalog # MAB304). The ND₅₀ is typically 0.03-0.1 µg/mL." class="big_thumb" />

204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-204-NA). The ND₅₀ is typically 0.02-0.1 µg/mL." class="big_lightbox"> 204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-204-NA). The ND₅₀ is typically 0.02-0.1 µg/mL." alt="Recombinant Human IL-4 (204-IL) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Human IL-4 (0.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-204-NA). The ND₅₀ is typically 0.02-0.1 µg/mL." class="big_thumb" />

204-IL) was serially diluted and captured by Mouse Anti-Human IL‑4 Monoclonal Antibody (Catalog # MAB604) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-204-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 204-IL) was serially diluted and captured by Mouse Anti-Human IL‑4 Monoclonal Antibody (Catalog # MAB604) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-204-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

654-P4) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-4 (2 ng/mL) is neutralized (green line) by increasing concen-trations of Porcine IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox"> 654-P4) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-4 (2 ng/mL) is neutralized (green line) by increasing concen-trations of Porcine IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654). The ND₅₀ is typically 1-5 µg/mL." alt="Recombinant Porcine IL-4 (Catalog # 654-P4) stimulates proliferation in the TF-1 human erythroleukemic cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Porcine IL-4 (2 ng/mL) is neutralized (green line) by increasing concen-trations of Porcine IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

654-P4) was serially diluted and captured by Goat Anti-Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 654-P4) was serially diluted and captured by Goat Anti-Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

654-P4) was serially diluted and captured by Goat Anti-Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 654-P4) was serially diluted and captured by Goat Anti-Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Porcine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF654) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

404-ML) stimulates proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-404-NA). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_lightbox"> 404-ML) stimulates proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-404-NA). The ND₅₀ is typically 0.1-0.3 µg/mL." alt="Recombinant Mouse IL‑4 (404-ML) stimulates proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-404-NA). The ND₅₀ is typically 0.1-0.3 µg/mL." class="big_thumb" />

404-ML) was serially diluted and captured by Rat Anti-Mouse IL‑4 Monoclonal Antibody (Catalog # MAB404) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-404-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 404-ML) was serially diluted and captured by Rat Anti-Mouse IL‑4 Monoclonal Antibody (Catalog # MAB404) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-404-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-4 (4 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA). The ND₅₀ is typically 0.05-015 µg/mL in the presence of PHA (10 µg/mL)." class="big_lightbox"> 504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-4 (4 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA). The ND₅₀ is typically 0.05-015 µg/mL in the presence of PHA (10 µg/mL)." alt="Recombinant Rat IL-4 (Catalog # 504-RL) stimulates proliferation in the rat splenocytes in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Rat IL-4 (4 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Rat IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA). The ND₅₀ is typically 0.05-015 µg/mL in the presence of PHA (10 µg/mL)." class="big_thumb" />

504-RL) was serially diluted and captured by Mouse Anti-Rat IL‑4 Monoclonal Antibody (Catalog # MAB504) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 504-RL) was serially diluted and captured by Mouse Anti-Rat IL‑4 Monoclonal Antibody (Catalog # MAB504) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." alt="IL-4 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Equine IL-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1809) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (yellow; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

1809-EL) was serially diluted and captured by Goat Anti-Equine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1809) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1809) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1809-EL) was serially diluted and captured by Goat Anti-Equine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1809) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1809) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

1809-EL) was serially diluted and captured by Goat Anti-Equine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1809) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1809) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 1809-EL) was serially diluted and captured by Goat Anti-Equine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1809) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Equine IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1809) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

504-RL) was serially diluted and captured by Mouse Anti-Rat IL‑4 Monoclonal Antibody (Catalog # MAB504) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 504-RL) was serially diluted and captured by Mouse Anti-Rat IL‑4 Monoclonal Antibody (Catalog # MAB504) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Rat IL‑4 (Catalog # 504-RL) was serially diluted and captured by Mouse Anti-Rat IL‑4 Monoclonal Antibody (Catalog # MAB504) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Rat IL‑4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-504-NA) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

404-ML) stimulates proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA). The ND₅₀ is typically 3-5 µg/mL." class="big_lightbox"> 404-ML) stimulates proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA). The ND₅₀ is typically 3-5 µg/mL." alt="Recombinant Mouse IL‑4 (Catalog # 404-ML) stimulates proliferation in the HT‑2 mouse T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Mouse IL‑4 (7.5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL‑4 Polyclonal Antibody (Catalog # AB-404-NA). The ND₅₀ is typically 3-5 µg/mL." class="big_thumb" />