IL-3R alpha/CD123 Antibody (107D2.08) - Azide and BSA Free

Immunohistochemistry: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100] - IHC staining of Bouin Paraffin section ofhuman invasive breast tumor with107D2.08 (DAB brown)

Flow Cytometry: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100] - Facs staining of CD11c-sorted tonsillar cells

Immunohistochemistry-Paraffin: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100] - Paraffin embedded breast cancer tissue sections

Immunohistochemistry-Paraffin: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100]

Immunohistochemistry: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100] - IHC staining of frozen humantonsilsection with 106C2.02(Fast Blue)

Flow Cytometry: IL-3 R alpha/CD123 Antibody (107D2.08) [DDX0300P-100] - Human PBMC enriched in monocytes and dendriticcells (Percollgradient) and analyzed by surface flowcytometry

• Reactivity: Hu, Ca
• Applications: WB, Flow, IHC, IP
• Clone: 107D2.08
• Clonality: Monoclonal
• Host: Mouse
• Conjugate: Unconjugated
• Format: Azide and BSA Free
• Concentration: 0.5 mg/ml

IL-3R alpha/CD123 Antibody (107D2.08) - Azide and BSA Free Summary

• Description: Novus Biologicals Mouse IL-3R alpha/CD123 Antibody (107D2.08) - Azide and BSA Free (DDX0300P-100) is a monoclonal antibody validated for use in IHC, WB, Flow and IP. Anti-IL-3R alpha/CD123 Antibody: Cited in 11 publications. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: IL3R Alpha/ CD123 was used as immunogen.
• Isotype: IgG1
• Clonality: Monoclonal
• Host: Mouse
• Gene: IL3RA
• Purity: Ion exchange chromatography

Applications/Dilutions

• Dilutions:
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:10-1:500
  • Immunohistochemistry-Frozen 1:10-1:500
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:10-1:500
  • Western Blot 1:100-1:2000

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Tris-NaCl (pH 8.0)
• Preservative: No Preservative
• Concentration: 0.5 mg/ml
• Purity: Ion exchange chromatography

Notes

This product is manufactured by Eurobio Scientific (formerly Dendritics) and distributed by Novus Biologicals.

Alternate Names for IL-3R alpha/CD123 Antibody (107D2.08) - Azide and BSA Free

• CD123 antigen
• CD123
• hIL3Ra
• hIL-3Ra
• IL-3 R alpha
• IL-3 receptor subunit alpha
• IL3R alpha
• IL-3R alpha
• IL-3R subunit alpha
• IL3R
• IL3RA
• IL-3Ra
• IL-3R-alpha
• IL3RAY
• IL3RX
• IL3RY
• interleukin 3 receptor, alpha (low affinity)
• interleukin-3 receptor subunit alpha
• MGC34174

Background

IL3RA (CD123) is a member of the immunoglobulin superfamily that is expressed on hematopoietic progenitors, basophils, mast cells, and megakaryocytes. This transmembrane glycoprotein can bind IL3 with low affinity but cannot transduce signals without association with additional protein partners. CD123 can complex with either common beta chain (CDw131) or the IL3RB chain to form high affinity heterodimeric IL3 receptors. CDw131 can complex with the Alpha subunits of the mouse IL3R, IL5R and GMCSFR to form high affinity receptors, while the IL3RB subunit is specific for IL3 but binds with low affinity. IL3 binding to the receptor complex can induce proliferation and differentiation of hematopoietic cells. It is present in Hematopoietic progenitors, basophils, mast cells and megakaryocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-3R alpha/CD123 Antibody (DDX0300P-100)(11)

Publications using DDX0300P-100

• Pang L, Ng KT, Liu J et al. Plasmacytoid dendritic cells recruited by HIF-1 alpha /eADO/ADORA1 signaling induce immunosuppression in hepatocellular carcinoma Cancer letters 2021-09-16 [PMID: 34536555] (IHC-P, Human)
• Broughton SE et al. Dual mechanism of interleukin-3 receptor blockade by an anti-cancer antibody. Cell Rep 2014-07-01 [PMID: 25043189]
• Koehl U et al. Clinical grade purification and expansion of NK cell products for an optimized manufacturing protocol. Front Oncol 2013-05-01 [PMID: 23730623]
• Brehm C et al. IL-2 stimulated but not unstimulated NK cells induce selective disappearance of peripheral blood cells: concomitant results to a phase I/II study. PLoS One 2011-01-01 [PMID: 22096557]
• Matsuo T et al. Bilateral conjunctival lesions in blastic plasmacytoid dendritic cell neoplasm. J Clin Exp Hematop 2011-01-01 [PMID: 21628861]
• Rochereau N et al. Phenotypic localization of distinct DC subsets in mouse Peyer Patch. Vaccine 2011-05-09 [PMID: 21439318]
• van de Laar L et al. A nonredundant role for canonical NF-kB in human myeloid dendritic cell development and function. J Immunol 2010-12-15 [PMID: 21076069]
• Bachmann M et al. Early production of IL-22 but not IL-17 by peripheral blood mononuclear cells exposed to live Borrelia burgdorferi: the role of monocytes and interleukin-1. PLoS Pathog 2010-10-01 [PMID: 20976193]
• Tsoukimadou M et al. Isolation of myeloid and plasmacytoid dendritic cells from human bronchoalveolar lavage fluid. Immunol Cell Biol 2006-06-01 [PMID: 16509829]
• Treilleux I et al. Dendritic cell infiltration and prognosis of early stage breast cancer. Clin Cancer Res 2004-11-01 [PMID: 15569976]
• Movassagh M et al. Selective accumulation of mature DC-Lamp+ dendritic cells in tumor sites is associated with efficient T-cell-mediated antitumor response and control of metastatic dissemination in melanoma. Cancer Res 2004-03-15 [PMID: 15026362]

Bioinformatics

• Gene Symbol: IL3RA