Western Blot: IL-23 R Antibody [NB600-1147] - Analysis of IL-23R in (A) Jurkat and (B) NIH 3T3 cells. 1:500 dilution.
Immunohistochemistry-Paraffin: IL-23 R Antibody [NB600-1147] - Staining of IL-23 Receptor in Human periarterial lymphatic sheath lymphocytes (spleen) at 10 ug/ml. Hematoxylin-eosin counterstain.
Western Blot: IL-23 R Antibody [NB600-1147] - Analysis using IL-23R antibody. Lysate from (A) human Jurkat and (B) Mouse NIH 3T3 cells probed with IL-23R antibody at 0.5 ug/ml.
Immunohistochemistry-Paraffin: IL-23 R Antibody [NB600-1147] - Analysis of Mouse spleen tissue probed with isotype control antibody (top) and IL-23R antibody (bottom) at 5 ug/ml.
Simple Western: IL-23 R Antibody [NB600-1147] - Lane view shows a specific band for IL23 Receptor in 0.5 mg/ml of NIH-3T3 lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation ...read more
Flow (Cell Surface) reported in scientific literature (PMID 24358364)
Flow Cytometry 1:10-1:1000
Immunohistochemistry 5-10 ug/ml
Immunohistochemistry-Paraffin 5-10 ug/ml
Simple Western 1:10
Western Blot 0.5-1.0 ug/ml
Based on immunogen sequence, this antibody will recognize the 629 amino acid (approx. 68 KDa) , 390 AA (approx. 42 KDa) and 227 AA (approx. 26 KDa) forms of human IL-23R. Flow (Cell Surface): See Kortylweski et al, 2009 for details.
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.
72 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.05% Sodium Azide
Protein G purified
Alternate Names for IL-23R Antibody
interleukin 23 receptor
IL-23 is a heterodimeric cytokine that shares biological properties with proinflammatory cytokines. IL-23 is composed of the IL-12p40 soluble receptor subunit and a novel cytokine-like subunit related to IL-12p35, termed p19. IL-23 mediates it cellular function by binding to IL-23R, which pairs with IL-12Rb1 to confer IL-23 responsiveness. However, human IL-12 does not bind to human IL-23R. IL-23 has been shown to possess biological activities on T cells that are similar as well distinct from those of IL-12.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Previously blocked with BSA 6%+2% normal goat serum. Secondary antibody used: Goat anti-rabbit alexa fluor 546 (Molecular Probes) 1/500. It works pretty well althought there is some non-specific signal mainly associated to the tissue-autofluorescence.
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