ROR gamma/RORC/NR1F3 Antibody - BSA Free

Images

 
Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Total protein from Human and mouse Thymus, Jurkat and U2OS cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunocytochemistry/ Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - U-2 OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Tissue section of human lymph node using anti- ROR gamma/RORC/NR1F3 antibody. The staining was developed with HRP labeled secondary antibody ...read more
Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Analysis of human ROR gamma/RORC/NR1F3 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide, 3) in 3T3, and 4) in RAW cell lysate using ...read more
Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Analysis of human testis using NBP2-24503.

Product Details

Summary
Reactivity Hu, Pm-OrSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Format
BSA Free
Concentration
1.0 mg/ml

Order Details

ROR gamma/RORC/NR1F3 Antibody - BSA Free Summary

Immunogen
A portion of amino acids 1-50 of human ROR gamma/RORC/NR1F3 was used as the immunogen.
Specificity
100% homologous in human isoforms CRA_a).
Predicted Species
Orangutan (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RORC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 2.5-10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
  • Immunoprecipitation
  • Western Blot 0.5-2 ug/ml
Application Notes
Use in immunoprecipitation reported in scientific literature (PMID:31451788).
Reviewed Applications
Read 1 Review rated 4
using
NBP2-24503 in the following applications:

Publications
Read Publications using
NBP2-24503 in the following applications:

  • 1 publication
  • IHC
    1 publication
  • IP
    1 publication
  • WB
    1 publication

Reactivity Notes

The amino acid sequence used as immunogen Porcine, Rat (81%).

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ROR gamma/RORC/NR1F3 Antibody - BSA Free

  • anti-ROR gamma
  • MGC129539
  • NR1F3
  • Nuclear receptor ROR gamma
  • Nuclear receptor ROR-gamma
  • Nuclear receptor RZR gamma
  • Nuclear receptor RZR-gamma
  • Nuclear receptor subfamily 1 group F member 3
  • RAR-Related Orphan Nuclear Receptor Variant 2
  • RAR-related orphan receptor C
  • RAR-related orphan receptor gamma
  • retinoic acid-binding receptor gamma
  • retinoid-related orphan receptor gamma
  • Retinoid-related orphan receptor-gamma
  • ROR gamma
  • RORC
  • RORG
  • RORGMGC129539
  • RZRG
  • RZRGRZR-GAMMA
  • TOR

Background

ROR gamma/RORC/NR1F3, aka RAR Orphan Receptor gamma, is a Nuclear Receptor 1 Thyroid Hormone-Like receptor containing a short A-T rich sequence follow by a single core motif half-site 5'-AGGTCA-3'. RORC is a key regulator of thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. NR1F3 regulates intrinsic transcriptional functions. Unlike other major nuclear receptors, RORC binds as a monomer to hormone response elements and is documented in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle. An N-terminal isoform of ROR gamma, ROR gamma T, inhibits Fas ligand expression and cytokine secretion in immature thymocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

NB400-111
Species: Hu, Mu(-)
Applications: ICC/IF, IHC, IHC-Fr, WB
NB600-607
Species: Hu, Rt
Applications: ELISA, IHC, IHC-P, NULL, WB
DY421
Species: Mu
Applications: ELISA
NBP1-89865
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
782-IL/CF
Species: Hu
Applications: BA
AF887
Species: Hu, Mu, Rt
Applications: CyTOF-ready, IHC, ICFlow, Simple Western, WB
D6050
Species: Hu
Applications: ELISA
NB100-1595
Species: Hu, Mu
Applications: IHC, IHC-P, IP, WB
MAB1417
Species: Bv, Hu, Mu
Applications: CyTOF-ready, ICC, IHC, ICFlow
NBP1-19371
Species: Ca, Hu, Mu, Po, Rb, Rt
Applications: Dual ISH-IHC, Flow, ICC/IF, IHC, IHC-Fr, IHC-P, Simple Western, WB
NBP2-22356
Species: Hu, Mu
Applications: ICC/IF, IHC, IHC-P, Simple Western, WB
NBP2-89142
Species: Hu, Mu, Rt
Applications: Flow, ICC/IF, IHC, IHC-P, WB
NB600-1049
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NBP1-87799
Species: Hu, Mu, Rt
Applications: ICC/IF, IHC, IHC-P, WB
NB100-39002
Species: Bv, Hu, Mu, Rt
Applications: Dual ISH-IHC, Flow, IB, ICC/IF, IHC, IHC-P, IP, Single-Cell Western, WB
NBP2-74653
Species: Hu, Mu, Rt
Applications: IHC, WB
AF9024
Species: Mu, Rt
Applications: IHC, WB
NBP2-13961
Species: Hu
Applications: ICC/IF, IHC, IHC-P, WB
NBP2-54301
Species: Hu, Mu, Rt
Applications: CyTOF-ready, ELISA, IHC, IHC-P, WB
NB200-157
Species: Hu
Applications: IHC, IHC-P, KO, WB
Supplier Logo

Publications for ROR gamma/RORC/NR1F3 Antibody (NBP2-24503)(2)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 4 applications: ICC/IF, IHC, IP, WB.


Filter By Application
ICC/IF
(1)
IHC
(1)
IP
(1)
WB
(1)
All Applications
Filter By Species
Human
(1)
All Species

Review for ROR gamma/RORC/NR1F3 Antibody (NBP2-24503) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24503:
Filter by Applications
WB
(1)
All Applications
Filter by Species
Human
(1)
All Species
Images Ratings Applications Species Date Details
Western Blot ROR gamma/RORC/NR1F3 NBP2-24503
Enlarge
4
reviewed by:
Verified Customer
WB Human 10/03/2014
View

Summary

ApplicationWestern Blot
Sample TestedHuman and hamster melanoma
SpeciesHuman
Lot12012958C-04
CommentsMany other non specific bands.

Blocking

Blocking Details5% skim milk in TBST 2 h, RT

Primary Anitbody

Dilution Ratio1:500 in 5% milk diluted TBST, overnight at 4 C

Secondary Antibody

Secondary Descriptiondonkey anti rabbit IgG-HRP
Secondary Manufacturer Cat#(sc-2077, Santa Cruz)
Secondary Concentration1:10,000

Details

Detection NotesECL, WestPico for 5 min, Positive Ctr=Hepatoma overexpressing ROR gamma and LNCaP

Comments

CommentsMany other non specific bands.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ROR gamma/RORC/NR1F3 Antibody (NBP2-24503). (Showing 1 - 4 of 4 FAQs).

  1. Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?
    • Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.
  2. What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?
    • The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.
  3. I would like to know if it was raised against the N terminus region or not, because RORgamma differs from RORgammaT since RORgammaT lacks a N terminus region that RORgamma has.
    • I can confirm that our product was raised against the N-terminal region (between aa 1-50) of the human RORC sequence found here: Uniprot site.
  4. Have you tested NBP2-24503 for cross-reactivity of the T-isoform? If so, can you send any related data you may have?
    • The NBP2-24503 is made to a region that is mostly missing on the RORgT isoform, so theoretically, there should be minimal cross reaction. But it does not look like we have any concrete testing to show that. Also, it does look like there is a secondary band in our testing that may be this isoform.

Secondary Antibodies

 

Isotype Controls

Additional ROR gamma/RORC/NR1F3 Products

Bioinformatics Tool for ROR gamma/RORC/NR1F3 Antibody (NBP2-24503)

Discover related pathways, diseases and genes to ROR gamma/RORC/NR1F3 Antibody (NBP2-24503). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for ROR gamma/RORC/NR1F3 Antibody (NBP2-24503)

Discover more about diseases related to ROR gamma/RORC/NR1F3 Antibody (NBP2-24503).
 

Pathways for ROR gamma/RORC/NR1F3 Antibody (NBP2-24503)

View related products by pathway.

PTMs for ROR gamma/RORC/NR1F3 Antibody (NBP2-24503)

Learn more about PTMs related to ROR gamma/RORC/NR1F3 Antibody (NBP2-24503).

Blogs on ROR gamma/RORC/NR1F3

There are no specific blogs for ROR gamma/RORC/NR1F3, but you can read our latest blog posts.
Omicron Variant Antibodies

Customers Who Bought This Also Bought

Contact Information

Download our Western Blot eHandbook

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

4
5
0
4
1
3
0
2
0
1
0

Verified Customer
10/03/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RORC
Uniprot