Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Total protein from Human and mouse Thymus, Jurkat and U2OS cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunocytochemistry/ Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - U-2 OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Tissue section of human lymph node using anti- ROR gamma/RORC/NR1F3 antibody. The staining was developed with HRP labeled secondary antibody ...read more
Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Analysis of human ROR gamma/RORC/NR1F3 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide, 3) in 3T3, and 4) in RAW cell lysate using ...read more
Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Analysis of human testis using NBP2-24503.
The amino acid sequence used as immunogen Porcine, Rat (81%).
Packaging, Storage & Formulations
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
0.02% Sodium Azide
Immunogen affinity purified
Alternate Names for ROR gamma/RORC/NR1F3 Antibody
Nuclear receptor ROR gamma
Nuclear receptor ROR-gamma
Nuclear receptor RZR gamma
Nuclear receptor RZR-gamma
Nuclear receptor subfamily 1 group F member 3
RAR-Related Orphan Nuclear Receptor Variant 2
RAR-related orphan receptor C
RAR-related orphan receptor gamma
retinoic acid-binding receptor gamma
retinoid-related orphan receptor gamma
Retinoid-related orphan receptor-gamma
ROR gamma/RORC/NR1F3, aka RAR Orphan Receptor gamma, is a Nuclear Receptor 1 Thyroid Hormone-Like receptor containing a short A-T rich sequence follow by a single core motif half-site 5'-AGGTCA-3'. RORC is a key regulator of thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. NR1F3 regulates intrinsic transcriptional functions. Unlike other major nuclear receptors, RORC binds as a monomer to hormone response elements and is documented in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle. An N-terminal isoform of ROR gamma, ROR gamma T, inhibits Fas ligand expression and cytokine secretion in immature thymocytes.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
FAQs for ROR gamma/RORC/NR1F3 Antibody (NBP2-24503). (Showing 1 - 4 of 4 FAQs).
Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?
Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.
What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?
The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.
I would like to know if it was raised against the N terminus region or not, because RORgamma differs from RORgammaT since RORgammaT lacks a N terminus region that RORgamma has.
I can confirm that our product was raised against the N-terminal region (between aa 1-50) of the human RORC sequence found here: Uniprot site.
Have you tested NBP2-24503 for cross-reactivity of the T-isoform? If so, can you send any related data you may have?
The NBP2-24503 is made to a region that is mostly missing on the RORgT isoform, so theoretically, there should be minimal cross reaction. But it does not look like we have any concrete testing to show that. Also, it does look like there is a secondary band in our testing that may be this isoform.
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Bio-Techne appreciates the critical role that you and our products and services play in research efforts to further scientific innovation and discovery. We are continually assessing our manufacturing and supplier capabilities during the COVID-19 situation and are implementing precautionary measures to ensure uninterrupted supply of products and services. Currently, and as we abide by local shelter in place orders across the world, we are fully operational and do not anticipate any material supply disruptions across our Bio-Techne brands and product lines. As the situation evolves, our goal is to utilize preventive measures to reduce the threat that COVID-19 poses to our ability to meet the needs of our customers globally.