ROR gamma/RORC/NR1F3 Antibody

Images

 
Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Total protein from Human and mouse Thymus, Jurkat and U2OS cells was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat ...read more
Immunocytochemistry/ Immunofluorescence: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - U-2 OS cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X TBS + 0.5% Triton-X100. The ...read more
Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Tissue section of human lymph node using anti- ROR gamma/RORC/NR1F3 antibody. The staining was developed with HRP labeled secondary antibody ...read more
Western Blot: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Analysis of human ROR gamma/RORC/NR1F3 in Jurkat cell lysate in the 1) absence, 2) presence of immunizing peptide, 3) in 3T3, and 4) in RAW cell lysate using ...read more
Immunohistochemistry-Paraffin: ROR gamma/RORC/NR1F3 Antibody [NBP2-24503] - Analysis of human testis using NBP2-24503.

Product Details

Summary
Reactivity Hu, OrSpecies Glossary
Applications WB, ICC/IF, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
1.0 mg/ml

ROR gamma/RORC/NR1F3 Antibody Summary

Immunogen
A portion of amino acids 1-50 of human ROR gamma/RORC/NR1F3 was used as the immunogen.
Specificity
100% homologous in human isoforms CRA_a).
Predicted Species
Orangutan (90%). Backed by our 100% Guarantee.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
RORC
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Immunocytochemistry/Immunofluorescence
  • Immunohistochemistry 2.5-10 ug/ml
  • Immunohistochemistry-Paraffin 10 ug/ml
Reviewed Applications
Read 1 Review rated 4
using
NBP2-24503 in the following applications:

Publications
Read Publication using
NBP2-24503 in the following applications:

Reactivity Notes

The amino acid sequence used as immunogen is 81% homologous in porcine, 81% in rat.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS
Preservative
0.02% Sodium Azide
Concentration
1.0 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ROR gamma/RORC/NR1F3 Antibody

  • anti-ROR gamma
  • MGC129539
  • NR1F3
  • Nuclear receptor ROR gamma
  • Nuclear receptor ROR-gamma
  • Nuclear receptor RZR gamma
  • Nuclear receptor RZR-gamma
  • Nuclear receptor subfamily 1 group F member 3
  • RAR-Related Orphan Nuclear Receptor Variant 2
  • RAR-related orphan receptor C
  • RAR-related orphan receptor gamma
  • retinoic acid-binding receptor gamma
  • retinoid-related orphan receptor gamma
  • Retinoid-related orphan receptor-gamma
  • ROR gamma
  • RORC
  • RORG
  • RORGMGC129539
  • RZRG
  • RZRGRZR-GAMMA
  • TOR

Background

ROR gamma/RORC/NR1F3, aka RAR Orphan Receptor gamma, is a Nuclear Receptor 1 Thyroid Hormone-Like receptor containing a short A-T rich sequence follow by a single core motif half-site 5'-AGGTCA-3'. RORC is a key regulator of thymopoiesis, bone metabolism, T-cell apoptosis, and lymphoid organogenesis. NR1F3 regulates intrinsic transcriptional functions. Unlike other major nuclear receptors, RORC binds as a monomer to hormone response elements and is documented in mouse thymus, adipose, bone, skeletal muscle, liver, kidney, and human skeletal muscle. An N-terminal isoform of ROR gamma, ROR gamma T, inhibits Fas ligand expression and cytokine secretion in immature thymocytes.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for ROR gamma/RORC/NR1F3 Antibody (NBP2-24503)(1)

We have publications tested in 1 confirmed species: Human.

We have publications tested in 2 applications: ICC/IF, IHC.


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ICC/IF
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IHC
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Review for ROR gamma/RORC/NR1F3 Antibody (NBP2-24503) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NBP2-24503:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot ROR gamma/RORC/NR1F3 NBP2-24503
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reviewed by:
Anonymous
WB Human 10/03/2014
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Summary

ApplicationWestern Blot
Sample TestedHuman and hamster melanoma
SpeciesHuman
Lot12012958C-04
CommentsMany other non specific bands.

Blocking

Blocking Details5% skim milk in TBST 2 h, RT

Primary Anitbody

Dilution Ratio1:500 in 5% milk diluted TBST, overnight at 4 C

Secondary Antibody

Secondary Descriptiondonkey anti rabbit IgG-HRP
Secondary Manufacturer Cat#(sc-2077, Santa Cruz)
Secondary Concentration1:10,000

Details

Detection NotesECL, WestPico for 5 min, Positive Ctr=Hepatoma overexpressing ROR gamma and LNCaP

Comments

CommentsMany other non specific bands.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for ROR gamma/RORC/NR1F3 Antibody (NBP2-24503). (Showing 1 - 4 of 4 FAQ).

  1. Does ROR gamma/RORC/NR1F3 antibodies comes in lyophilized form?
    • Yes, we have 3 RORC antibodies delivere in lyophilized form: MAB61091, MAB61092, MAB6109.
  2. What the theoretical molecular weight for ROR gamma/RORC/NR1F3 antibodies?
    • The TMW of ROR gamma/RORC/NR1F3 antibodies is approximately 56 - 60.
  3. I would like to know if it was raised against the N terminus region or not, because RORgamma differs from RORgammaT since RORgammaT lacks a N terminus region that RORgamma has.
    • I can confirm that our product was raised against the N-terminal region (between aa 1-50) of the human RORC sequence found here: Uniprot site.
  4. Have you tested NBP2-24503 for cross-reactivity of the T-isoform? If so, can you send any related data you may have?
    • The NBP2-24503 is made to a region that is mostly missing on the RORgT isoform, so theoretically, there should be minimal cross reaction. But it does not look like we have any concrete testing to show that. Also, it does look like there is a secondary band in our testing that may be this isoform.

Secondary Antibodies

 

Isotype Controls

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Recent Reviews

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Anonymous
10/03/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol RORC
Uniprot