Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il12b

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA, 15 Plate

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Publications for IL-12/IL-23 p40 (DY499)(54)

Publications using DY499

• Chin, J;Abeydeera, N;Repasy, T;Rivera-Lugo, R;Mitchell, G;Nguyen, VQ;Zheng, W;Richards, A;Stevenson, E;Swaney, DL;Krogan, NJ;Ernst, JD;Cox, JS;Budzik, JM; Mycobacterium tuberculosis triggers reduced inflammatory cytokine responses and virulence in mice lacking Tax1bp1 PLoS pathogens 2025-10-01 [PMID: 41171885] (Mouse)
• Gregg, B;Guerra, AJ;Raverty, SA;Sardinha-Silva, A;Kafsack, BFC;Schultz, TL;Gurczynski, SJ;Moore, BB;Carruthers, VB;Grigg, ME; Toxoplasma induced cytokine release syndrome is critically dependent on the expression of pore-forming Perforin-Like Protein-1 bioRxiv : the preprint server for biology 2025-03-17 [PMID: 40166190] (Mouse, Transgenic Mouse)
• Chin, J;Abeydeera, N;Repasy, T;Rivera-Lugo, R;Mitchell, G;Nguyen, VQ;Zheng, W;Richards, A;Swaney, DL;Krogan, NJ;Ernst, JD;Cox, JS;Budzik, JM; Tax1bp1 enhances bacterial virulence and promotes inflammatory responses during Mycobacterium tuberculosis infection of alveolar macrophages bioRxiv : the preprint server for biology 2024-12-16 [PMID: 39763950] (Transgenic Mouse)
• Lee, J;Kim, MY;Kim, HJ;Choi, WS;Kim, HS; Impaired autophagy in myeloid cells aggravates psoriasis-like skin inflammation through the IL-1?/CXCL2/neutrophil axis Cell & bioscience 2024-05-04 [PMID: 38704587] (Transgenic Mouse)
• S Papoutsopo, L Pollock, JM Williams, MMLF Abdul-Mahd, R Dobbash, CA Duckworth, BJ Campbell Interleukin-10 Deficiency Impacts on TNF-Induced NFkappaB Regulated Responses In Vivo Biology, 2022-09-20;11(10):. 2022-09-20 [PMID: 36290283] (Mouse, Transgenic Mouse)
• SY Ahn, CTT Le, EJ Ko Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Ovalbumin-Specific Cell Mediated Immunity in Mice Model Biology, 2021-09-13;10(9):. 2021-09-13 [PMID: 34571785] (Mouse)
• CC Lin, YR Shen, CC Chang, XY Guo, YY Young, TY Lai, IS Yu, CY Lee, TH Chuang, HY Tsai, LC Hsu Terminal uridyltransferase 7 regulates TLR4-triggered inflammation by controlling Regnase-1 mRNA uridylation and degradation Nature Communications, 2021-06-29;12(1):3878. 2021-06-29 [PMID: 34188032] (Mouse)
• MN Rana, J Lu, E Xue, J Ruan, Y Liu, L Zhang, R Dhar, Y Li, Z Hu, J Zhou, W Ma, H Tang PDE9 Inhibitor PF-04447943 Attenuates DSS-Induced Colitis by Suppressing Oxidative Stress, Inflammation, and Regulating T-Cell Polarization Frontiers in Pharmacology, 2021-04-08;12(0):643215. 2021-04-08 [PMID: 33967779] (Mouse)
• S Ribes, L Zacke, S Nessler, N Saiepour, E Avendaño-G, M Ballüer, UK Hanisch, R Nau Oligodeoxynucleotides containing unmethylated cytosine-guanine motifs are effective immunostimulants against pneumococcal meningitis in the immunocompetent and neutropenic host Journal of Neuroinflammation, 2021-02-02;18(1):39. 2021-02-02 [PMID: 33531028] (Mouse)
• DC Montrose, R Nishiguchi, S Basu, HA Staab, XK Zhou, H Wang, L Meng, M Johncilla, JR Cubillos-R, DK Morales, MT Wells, KW Simpson, S Zhang, B Dogan, C Jiao, Z Fei, A Oka, JW Herzog, RB Sartor, AJ Dannenberg Dietary Fructose Alters the Composition, Localization and Metabolism of Gut Microbiota in Association with Worsening Colitis Cell Mol Gastroenterol Hepatol, 2020-09-20;0(0):. 2020-09-20 [PMID: 32961355] (Mouse)
• ZS Wong, SL Sokol-Borr, P Olias, JP Dubey, JP Boyle Head-to-head comparisons of Toxoplasma gondii and its near relative Hammondia hammondi reveal dramatic differences in the host response and effectors with species-specific functions PLoS Pathog., 2020-06-23;16(6):e1008528. 2020-06-23 [PMID: 32574210] (Mouse)
• KD Curtis, PR Smith, HW Despres, JP Snyder, TC Hogan, PD Rodriguez, E Amiel Glycogen Metabolism Supports Early Glycolytic Reprogramming and Activation in Dendritic Cells in Response to Both TLR and Syk-Dependent CLR Agonists Cells, 2020-03-14;9(3):. 2020-03-14 [PMID: 32183271] (Mouse)
• S Benmerzoug, B Bounab, S Rose, D Gosset, F Biet, T Cochard, A Xavier, N Rouxel, L Fauconnier, WGC Horsnell, B Ryffel, D Togbe, VFJ Quesniaux Sterile Lung Inflammation Induced by Silica Exacerbates Mycobacterium tuberculosis Infection via STING-Dependent Type 2 Immunity Cell Rep, 2019-05-28;27(9):2649-2664.e5. 2019-05-28 [PMID: 31141689] (Mouse)
• S Vander Bek, JC de Vries, B Meier-Schi, P Meyer, D Jiang, A Sindrilaru, FF Ferreira, A Hainzl, S Schatz, J Muschhamme, NJ Scheurmann, P Kampilafko, AM Seitz, L Dürselen, A Ignatius, MA Kluth, C Ganss, M Wlaschek, K Singh, P Maity, NY Frank, MH Frank, K Scharffett Newly defined ABCB5+ dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist Stem Cells, 2019-05-13;0(0):. 2019-05-13 [PMID: 31002437] (Mouse)
• E Engelowski, NF Modares, S Gorressen, P Bouvain, D Semmler, C Alter, Z Ding, U Flögel, J Schrader, H Xu, PA Lang, J Fischer, DM Floss, J Scheller IL-23R Signaling Plays No Role in Myocardial Infarction Sci Rep, 2018-11-20;8(1):17078. 2018-11-20 [PMID: 30459442] (Mouse)
• AP Gounder, CC Yokoyama, NN Jarjour, TL Bricker, BT Edelson, ACM Boon Interferon induced protein 35 exacerbates H5N1 influenza disease through the expression of IL-12p40 homodimer PLoS Pathog., 2018-04-26;14(4):e1007001. 2018-04-26 [PMID: 29698474] (Mouse)
• M Murase, T Kawasaki, R Hakozaki, T Sueyoshi, DDP Putri, Y Kitai, S Sato, M Ikawa, T Kawai Intravesicular Acidification Regulates Lipopolysaccharide Inflammation and Tolerance through TLR4 Trafficking J. Immunol., 2018-03-14;0(0):. 2018-03-14 [PMID: 29540576] (Mouse)
• SY Lin, SY Hsieh, YT Fan, WC Wei, PW Hsiao, DH Tsai, TS Wu, NS Yang Necroptosis promotes autophagy-dependent upregulation of DAMP and results in immunosurveillance Autophagy, 2017-12-31;0(0):0. 2017-12-31 [PMID: 29171784] (Mouse)
• TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice JCI Insight, 2017-05-18;2(10):. 2017-05-18 [PMID: 28515358] (Mouse)
• M Lynch, TA Walsh, I Marszalows, AE Webb, M MacAogain, TR Rogers, H Windle, D Kelleher, MJ O'Connell, CE Loscher Surface layer proteins from virulent Clostridium difficile ribotypes exhibit signatures of positive selection with consequences for innate immune response BMC Evol. Biol, 2017-03-23;17(1):90. 2017-03-23 [PMID: 28335725] (Mouse)
• EL Ramos, SS Santana, MV Silva, FM Santiago, TW Mineo, JR Mineo Lectins from Synadenium carinatum (ScLL) and Artocarpus heterophyllus (ArtinM) Are Able to Induce Beneficial Immunomodulatory Effects in a Murine Model for Treatment of Toxoplasma gondii Infection Front Cell Infect Microbiol, 2016-11-25;6(0):164. 2016-11-25 [PMID: 27933277] (Mouse)
• MyD88 adapter-like (Mal)/TIRAP is required for cytokine production by splenic Ly6CloTLR2hi but not by Ly6ChiTLR2hi monocytes during Trypanosoma cruzi Infection J Biol Chem, 2016-09-19;0(0):. 2016-09-19 [PMID: 27646001] (Mouse)
• Alternatively Activated Mononuclear Phagocytes from the Skin Site of Infection and the Impact of IL-4R? Signalling on CD4+T Cell Survival in Draining Lymph Nodes after Repeated Exposure to Schistosoma mansoni Cercariae PLoS Negl Trop Dis, 2016-08-09;10(8):e0004911. 2016-08-09 [PMID: 27505056] (Mouse)
• Sha X, Meng S, Li X, Xi H, Maddaloni M, Pascual D, Shan H, Jiang X, Wang H, Yang X Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway. J Biol Chem, 2015-06-17;290(31):19307-18. 2015-06-17 [PMID: 26085094] (Mouse)
• Gillgrass A, Chew M, Krneta T, Ashkar A Overexpression of IL-15 promotes tumor destruction via NK1.1+ cells in a spontaneous breast cancer model. BMC Cancer, 2015-04-16;15(0):293. 2015-04-16 [PMID: 25879689] (Mouse)
• Schroder J, Moll J, Baran P, Grotzinger J, Scheller J, Floss D Non-canonical interleukin 23 receptor complex assembly: p40 protein recruits interleukin 12 receptor beta1 via site II and induces p19/interleukin 23 receptor interaction via site III. J Biol Chem, 2014-11-04;290(1):359-70. 2014-11-04 [PMID: 25371211] (Mouse)
• Haste L, Hulland K, Bolton S, Yesilkaya H, McKechnie K, Andrew P Development and characterization of a long-term murine model of Streptococcus pneumoniae infection of the lower airways. Infect Immun, 2014-05-27;82(8):3289-98. 2014-05-27 [PMID: 24866797] (Mouse)
• Ribes S, Meister T, Ott M, Redlich S, Janova H, Hanisch U, Nessler S, Nau R Intraperitoneal prophylaxis with CpG oligodeoxynucleotides protects neutropenic mice against intracerebral Escherichia coli K1 infection. J Neuroinflammation, 2014-01-23;11(0):14. 2014-01-23 [PMID: 24456653] (Mouse)
• Berod L, Stuve P, Swallow M, Arnold-Schrauf C, Kruse F, Gentilini M, Freitag J, Holzmann B, Sparwasser T MyD88 signalling in myeloid cells is sufficient to prevent chronic mycobacterial infection. Eur J Immunol, 2014-02-13;44(5):1399-409. 2014-02-13 [PMID: 24435955] (Mouse)
• Serezani CH, Kane S, Collins L Macrophage Dectin-1 Expression Is Controlled by Leukotriene B4 via a GM-CSF/PU.1 Axis. J. Immunol., 2012-06-13;189(2):906-15. 2012-06-13 [PMID: 22696442] (Mouse)
• Gowda NM, Wu X, Gowda DC TLR9 and MyD88 are crucial for the development of protective immunity to malaria. J. Immunol., 2012-04-18;188(10):5073-85. 2012-04-18 [PMID: 22516959] (Mouse)
• Barber DL, Mayer-Barber KD, Feng CG CD4 T cells promote rather than control tuberculosis in the absence of PD-1-mediated inhibition. J. Immunol., 2010-12-20;186(3):1598-607. 2010-12-20 [PMID: 21172867] (Mouse)
• Zhang X, Schmudde I, Laumonnier Y, Pandey MK, Clark JR, Konig P, Gerard NP, Gerard C, Wills-Karp M, Kohl J A critical role for C5L2 in the pathogenesis of experimental allergic asthma. J. Immunol., 2010-10-25;185(11):6741-52. 2010-10-25 [PMID: 20974988] (Mouse)
• Reynolds CM, Draper E, Keogh B, Rahman A, Moloney AP, Mills KH, Loscher CE, Roche HM A conjugated linoleic acid-enriched beef diet attenuates lipopolysaccharide-induced inflammation in mice in part through PPARgamma-mediated suppression of toll-like receptor 4. J. Nutr., 2009-10-21;139(12):2351-7. 2009-10-21 [PMID: 19846417] (Mouse)
• Wan H, Coppens JM, van Helden-Meeuwsen CG, Leenen PJ, Van Rooijen N, Khan NA, Kiekens RC, Benner R, Versnel MA Chorionic gonadotropin alleviates thioglycollate-induced peritonitis by affecting macrophage function. J. Leukoc. Biol., 2009-05-04;86(2):361-70. 2009-05-04 [PMID: 19414540] (Mouse)
• Lembo A, Pelletier M, Iyer R, Timko M, Dudda JC, West TE, Wilson CB, Hajjar AM, Skerrett SJ Administration of a synthetic TLR4 agonist protects mice from pneumonic tularemia. J. Immunol., 2008-06-01;180(11):7574-81. 2008-06-01 [PMID: 18490759] (Mouse)
• Schaefer M, Reiling N, Fessler C, Stephani J, Taniuchi I, Hatam F, Yildirim AO, Fehrenbach H, Walter K, Ruland J, Wagner H, Ehlers S, Sparwasser T Decreased pathology and prolonged survival of human DC-SIGN transgenic mice during mycobacterial infection. J. Immunol., 2008-05-15;180(10):6836-45. 2008-05-15 [PMID: 18453604] (Mouse)
• Hawn TR, Berrington WR, Smith IA, Uematsu S, Akira S, Aderem A, Smith KD, Skerrett SJ Altered inflammatory responses in TLR5-deficient mice infected with Legionella pneumophila. J. Immunol., 2007-11-15;179(10):6981-7. 2007-11-15 [PMID: 17982089] (Mouse)
• Higgins SC, Jarnicki AG, Lavelle EC, Mills KH TLR4 mediates vaccine-induced protective cellular immunity to Bordetella pertussis: role of IL-17-producing T cells. J. Immunol., 2006-12-01;177(11):7980-9. 2006-12-01 [PMID: 17114471] (Mouse)
• Rodriguez N, Wantia N, Fend F, Durr S, Wagner H, Miethke T Differential involvement of TLR2 and TLR4 in host survival during pulmonary infection with Chlamydia pneumoniae. Eur. J. Immunol., 2006-05-01;36(5):1145-55. 2006-05-01 [PMID: 16609927] (Mouse)
• Gilleron M, Nigou J, Nicolle D, Quesniaux V, Puzo G The acylation state of mycobacterial lipomannans modulates innate immunity response through toll-like receptor 2. Chem. Biol., 2006-01-01;13(1):39-47. 2006-01-01 [PMID: 16426970] (Mouse)
• Noulin N, Quesniaux VF, Schnyder-Candrian S, Schnyder B, Maillet I, Robert T, Vargaftig BB, Ryffel B, Couillin I Both hemopoietic and resident cells are required for MyD88-dependent pulmonary inflammatory response to inhaled endotoxin. J. Immunol., 2005-11-15;175(10):6861-9. 2005-11-15 [PMID: 16272344] (Mouse)
• Delale T, Paquin A, Asselin-Paturel C, Dalod M, Brizard G, Bates EE, Kastner P, Chan S, Akira S, Vicari A, Biron CA, Trinchieri G, Briere F MyD88-dependent and -independent murine cytomegalovirus sensing for IFN-alpha release and initiation of immune responses in vivo. J. Immunol., 2005-11-15;175(10):6723-32. 2005-11-15 [PMID: 16272328] (Mouse)
• Fremond CM, Yeremeev V, Nicolle DM, Jacobs M, Quesniaux VF, Ryffel B Fatal Mycobacterium tuberculosis infection despite adaptive immune response in the absence of MyD88. J. Clin. Invest., 2004-12-01;114(12):1790-9. 2004-12-01 [PMID: 15599404] (Mouse)
• Nicolle D, Fremond C, Pichon X, Bouchot A, Maillet I, Ryffel B, Quesniaux VJ Long-term control of Mycobacterium bovis BCG infection in the absence of Toll-like receptors (TLRs): investigation of TLR2-, TLR6-, or TLR2-TLR4-deficient mice. Infect. Immun., 2004-12-01;72(12):6994-7004. 2004-12-01 [PMID: 15557622] (Mouse)
• Zamboni DS, Campos MA, Torrecilhas AC, Kiss K, Samuel JE, Golenbock DT, Lauw FN, Roy CR, Almeida IC, Gazzinelli RT Stimulation of toll-like receptor 2 by Coxiella burnetii is required for macrophage production of pro-inflammatory cytokines and resistance to infection. J. Biol. Chem., 2004-10-13;279(52):54405-15. 2004-10-13 [PMID: 15485838] (Mouse)
• Chong MM, Thomas HE, Kay TW Severe pancreatitis with exocrine destruction and increased islet neogenesis in mice with suppressor of cytokine signaling-1 deficiency. Am. J. Pathol., 2004-09-01;165(3):913-21. 2004-09-01 [PMID: 15331415] (Mouse)
• Matsumoto M, Einhaus D, Gold ES, Aderem A Simvastatin augments lipopolysaccharide-induced proinflammatory responses in macrophages by differential regulation of the c-Fos and c-Jun transcription factors. J. Immunol., 2004-06-15;172(12):7377-84. 2004-06-15 [PMID: 15187114] (Mouse)
• Torres D, Barrier M, Bihl F, Quesniaux VJ, Maillet I, Akira S, Ryffel B, Erard F Toll-like receptor 2 is required for optimal control of Listeria monocytogenes infection. Infect. Immun., 2004-04-01;72(4):2131-9. 2004-04-01 [PMID: 15039335] (Mouse)
• Takahashi M, Kobayashi Y Cytokine production in association with phagocytosis of apoptotic cells by immature dendritic cells. Cell. Immunol., 2003-12-01;226(2):105-15. 2003-12-01 [PMID: 14962498] (Mouse)
• Gilleron M, Quesniaux VF, Puzo G Acylation state of the phosphatidylinositol hexamannosides from Mycobacterium bovis bacillus Calmette Guerin and mycobacterium tuberculosis H37Rv and its implication in Toll-like receptor response. J. Biol. Chem., 2003-05-29;278(32):29880-9. 2003-05-29 [PMID: 12775723] (Mouse)
• O'Keeffe M CpG-DNA aided cross-presentation of soluble antigens by dendritic cells. Eur. J. Immunol., 2002-08-01;32(8):2356-64. 2002-08-01 [PMID: 12209649] (Mouse)
• O'Garra A Reversal of tumor-induced dendritic cell paralysis by CpG immunostimulatory oligonucleotide and anti-interleukin 10 receptor antibody. J. Exp. Med., 2002-08-19;196(4):541-9. 2002-08-19 [PMID: 12186845] (Mouse)

FAQs for IL-12/IL-23 p40 (DY499). (Showing 1 - 1 of 1 FAQs).

1. What is the difference between the Quantikine and Duoset ELISA kits?
   • Our Quantikine kits are fully optimized and validated for the sample types listed on the product specific webpage and datasheet, as this can vary between kits. Each kit is supplied ready to use with one pre-coated 96-well plate, a detection antibody directly conjugated to HRP, and all other necessary reagents. These kits are ideal for researchers who want the convenience of a ready to use and optimized ELISA product. Some of these kits are also available prepackaged in larger 6 and 50 plate sizes.Our DuoSet Kits, in contrast, are ELISA development kits containing the capture and detection antibody, the mass-value calibrated standard, and streptavidin-HRP to prepare approximately 5 or 15 plates. Ancillary reagents will need to be used/purchased, and for most kits, we will recommend one of our Ancillary Reagent Kits, which contain the reagents we use ourselves in-house. DuoSet kits are validated only for cell culture supernatant samples and therefore require further development and validation for accurate measurement in more complex samples such as serum and plasma. Our DuoSet Kits offer an economical, flexible alternative for the experienced ELISA user.

Bioinformatics

• Gene Symbol: Il12b