Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA, 15 Plate

• Reactivity: Mu
• Applications: ELISA
• Conjugate: Biotin

Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA, 15 Plate Summary

• Source: N/A
• Assay Type: Solid Phase Sandwich ELISA
• Inter-Assay: See PDF Datasheet for details
• Intra-Assay: See PDF Datasheet for details
• Spike Recovery: See PDF Datasheet for details
• Sample Volume: See PDF Datasheet for details
• Gene: Il12b

Applications/Dilutions

• Dilutions:
  • ELISA
• Application Notes: No significant interference observed with available related molecules.

Packaging, Storage & Formulations

• Storage: Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Mouse IL-12/IL-23 p40 Allele-specific DuoSet ELISA, 15 Plate

• CLMF p40
• CLMF
• CLMF2
• Cytotoxic lymphocyte maturation factor 40 kDa subunit
• IL12 p40
• IL-12 p40
• IL-12 subunit p40
• IL12B
• IL-12B
• IL-12BNK cell stimulatory factor chain 2
• interleukin 12, p40
• interleukin 12B (natural killer cell stimulatory factor 2, cytotoxic lymphocytematuration factor 2, p40)
• interleukin-12 beta chain
• interleukin-12 subunit beta
• natural killer cell stimulatory factor, 40 kD subunit
• NKSF
• NKSF2
• NKSF2IL12, subunit p40

Background

Interleukin 12 (IL-12), also known as natural killer cell stimulatory factor (NKSF) or cytotoxic lymphocyte maturation factor (CLMF), is a heterodimeric pleiotropic cytokine made up of a 40 kDa (p40) subunit and a 35 kDa (p35) subunit. The IL-12 p40 subunit is shared by IL-23, another heterodimeric cytokine that has biological activities similar to, as well as distinct from, IL-12. IL-12 is produced by macrophages and B cells and has been shown to have multiple effects on T cells and natural killer (NK) cells. While mouse IL-12 is active on both human and mouse cells, human IL-12 is not active on mouse cells.

Publications for IL-12/IL-23 p40 (DY499)(53)

Publications using DY499

• Gregg, B;Guerra, AJ;Raverty, SA;Sardinha-Silva, A;Kafsack, BFC;Schultz, TL;Gurczynski, SJ;Moore, BB;Carruthers, VB;Grigg, ME; Toxoplasma induced cytokine release syndrome is critically dependent on the expression of pore-forming Perforin-Like Protein-1 bioRxiv : the preprint server for biology 2025-03-17 [PMID: 40166190] (Mouse, Transgenic Mouse)
• Chin, J;Abeydeera, N;Repasy, T;Rivera-Lugo, R;Mitchell, G;Nguyen, VQ;Zheng, W;Richards, A;Swaney, DL;Krogan, NJ;Ernst, JD;Cox, JS;Budzik, JM; Tax1bp1 enhances bacterial virulence and promotes inflammatory responses during Mycobacterium tuberculosis infection of alveolar macrophages bioRxiv : the preprint server for biology 2024-12-16 [PMID: 39763950] (Transgenic Mouse)
• Lee, J;Kim, MY;Kim, HJ;Choi, WS;Kim, HS; Impaired autophagy in myeloid cells aggravates psoriasis-like skin inflammation through the IL-1?/CXCL2/neutrophil axis Cell & bioscience 2024-05-04 [PMID: 38704587] (Transgenic Mouse)
• S Papoutsopo, L Pollock, JM Williams, MMLF Abdul-Mahd, R Dobbash, CA Duckworth, BJ Campbell Interleukin-10 Deficiency Impacts on TNF-Induced NFkappaB Regulated Responses In Vivo Biology, 2022-09-20;11(10):. 2022-09-20 [PMID: 36290283] (Mouse, Transgenic Mouse)
• SY Ahn, CTT Le, EJ Ko Monophosphoryl Lipid A and Poly I:C Combination Adjuvant Promoted Ovalbumin-Specific Cell Mediated Immunity in Mice Model Biology, 2021-09-13;10(9):. 2021-09-13 [PMID: 34571785] (Mouse)
• CC Lin, YR Shen, CC Chang, XY Guo, YY Young, TY Lai, IS Yu, CY Lee, TH Chuang, HY Tsai, LC Hsu Terminal uridyltransferase 7 regulates TLR4-triggered inflammation by controlling Regnase-1 mRNA uridylation and degradation Nature Communications, 2021-06-29;12(1):3878. 2021-06-29 [PMID: 34188032] (Mouse)
• MN Rana, J Lu, E Xue, J Ruan, Y Liu, L Zhang, R Dhar, Y Li, Z Hu, J Zhou, W Ma, H Tang PDE9 Inhibitor PF-04447943 Attenuates DSS-Induced Colitis by Suppressing Oxidative Stress, Inflammation, and Regulating T-Cell Polarization Frontiers in Pharmacology, 2021-04-08;12(0):643215. 2021-04-08 [PMID: 33967779] (Mouse)
• S Ribes, L Zacke, S Nessler, N Saiepour, E Avendaño-G, M Ballüer, UK Hanisch, R Nau Oligodeoxynucleotides containing unmethylated cytosine-guanine motifs are effective immunostimulants against pneumococcal meningitis in the immunocompetent and neutropenic host Journal of Neuroinflammation, 2021-02-02;18(1):39. 2021-02-02 [PMID: 33531028] (Mouse)
• DC Montrose, R Nishiguchi, S Basu, HA Staab, XK Zhou, H Wang, L Meng, M Johncilla, JR Cubillos-R, DK Morales, MT Wells, KW Simpson, S Zhang, B Dogan, C Jiao, Z Fei, A Oka, JW Herzog, RB Sartor, AJ Dannenberg Dietary Fructose Alters the Composition, Localization and Metabolism of Gut Microbiota in Association with Worsening Colitis Cell Mol Gastroenterol Hepatol, 2020-09-20;0(0):. 2020-09-20 [PMID: 32961355] (Mouse)
• ZS Wong, SL Sokol-Borr, P Olias, JP Dubey, JP Boyle Head-to-head comparisons of Toxoplasma gondii and its near relative Hammondia hammondi reveal dramatic differences in the host response and effectors with species-specific functions PLoS Pathog., 2020-06-23;16(6):e1008528. 2020-06-23 [PMID: 32574210] (Mouse)
• KD Curtis, PR Smith, HW Despres, JP Snyder, TC Hogan, PD Rodriguez, E Amiel Glycogen Metabolism Supports Early Glycolytic Reprogramming and Activation in Dendritic Cells in Response to Both TLR and Syk-Dependent CLR Agonists Cells, 2020-03-14;9(3):. 2020-03-14 [PMID: 32183271] (Mouse)
• S Benmerzoug, B Bounab, S Rose, D Gosset, F Biet, T Cochard, A Xavier, N Rouxel, L Fauconnier, WGC Horsnell, B Ryffel, D Togbe, VFJ Quesniaux Sterile Lung Inflammation Induced by Silica Exacerbates Mycobacterium tuberculosis Infection via STING-Dependent Type 2 Immunity Cell Rep, 2019-05-28;27(9):2649-2664.e5. 2019-05-28 [PMID: 31141689] (Mouse)
• S Vander Bek, JC de Vries, B Meier-Schi, P Meyer, D Jiang, A Sindrilaru, FF Ferreira, A Hainzl, S Schatz, J Muschhamme, NJ Scheurmann, P Kampilafko, AM Seitz, L Dürselen, A Ignatius, MA Kluth, C Ganss, M Wlaschek, K Singh, P Maity, NY Frank, MH Frank, K Scharffett Newly defined ABCB5+ dermal mesenchymal stem cells promote healing of chronic iron overload wounds via secretion of interleukin-1 receptor antagonist Stem Cells, 2019-05-13;0(0):. 2019-05-13 [PMID: 31002437] (Mouse)
• E Engelowski, NF Modares, S Gorressen, P Bouvain, D Semmler, C Alter, Z Ding, U Flögel, J Schrader, H Xu, PA Lang, J Fischer, DM Floss, J Scheller IL-23R Signaling Plays No Role in Myocardial Infarction Sci Rep, 2018-11-20;8(1):17078. 2018-11-20 [PMID: 30459442] (Mouse)
• AP Gounder, CC Yokoyama, NN Jarjour, TL Bricker, BT Edelson, ACM Boon Interferon induced protein 35 exacerbates H5N1 influenza disease through the expression of IL-12p40 homodimer PLoS Pathog., 2018-04-26;14(4):e1007001. 2018-04-26 [PMID: 29698474] (Mouse)
• M Murase, T Kawasaki, R Hakozaki, T Sueyoshi, DDP Putri, Y Kitai, S Sato, M Ikawa, T Kawai Intravesicular Acidification Regulates Lipopolysaccharide Inflammation and Tolerance through TLR4 Trafficking J. Immunol., 2018-03-14;0(0):. 2018-03-14 [PMID: 29540576] (Mouse)
• SY Lin, SY Hsieh, YT Fan, WC Wei, PW Hsiao, DH Tsai, TS Wu, NS Yang Necroptosis promotes autophagy-dependent upregulation of DAMP and results in immunosurveillance Autophagy, 2017-12-31;0(0):0. 2017-12-31 [PMID: 29171784] (Mouse)
• TB Oriss, M Raundhal, C Morse, RE Huff, S Das, R Hannum, MC Gauthier, KL Scholl, K Chakrabort, SM Nouraie, SE Wenzel, P Ray, A Ray IRF5 distinguishes severe asthma in humans and drives Th1 phenotype and airway hyperreactivity in mice JCI Insight, 2017-05-18;2(10):. 2017-05-18 [PMID: 28515358] (Mouse)
• M Lynch, TA Walsh, I Marszalows, AE Webb, M MacAogain, TR Rogers, H Windle, D Kelleher, MJ O'Connell, CE Loscher Surface layer proteins from virulent Clostridium difficile ribotypes exhibit signatures of positive selection with consequences for innate immune response BMC Evol. Biol, 2017-03-23;17(1):90. 2017-03-23 [PMID: 28335725] (Mouse)
• EL Ramos, SS Santana, MV Silva, FM Santiago, TW Mineo, JR Mineo Lectins from Synadenium carinatum (ScLL) and Artocarpus heterophyllus (ArtinM) Are Able to Induce Beneficial Immunomodulatory Effects in a Murine Model for Treatment of Toxoplasma gondii Infection Front Cell Infect Microbiol, 2016-11-25;6(0):164. 2016-11-25 [PMID: 27933277] (Mouse)
• MyD88 adapter-like (Mal)/TIRAP is required for cytokine production by splenic Ly6CloTLR2hi but not by Ly6ChiTLR2hi monocytes during Trypanosoma cruzi Infection J Biol Chem, 2016-09-19;0(0):. 2016-09-19 [PMID: 27646001] (Mouse)
• Alternatively Activated Mononuclear Phagocytes from the Skin Site of Infection and the Impact of IL-4R? Signalling on CD4+T Cell Survival in Draining Lymph Nodes after Repeated Exposure to Schistosoma mansoni Cercariae PLoS Negl Trop Dis, 2016-08-09;10(8):e0004911. 2016-08-09 [PMID: 27505056] (Mouse)
• Sha X, Meng S, Li X, Xi H, Maddaloni M, Pascual D, Shan H, Jiang X, Wang H, Yang X Interleukin-35 Inhibits Endothelial Cell Activation by Suppressing MAPK-AP-1 Pathway. J Biol Chem, 2015-06-17;290(31):19307-18. 2015-06-17 [PMID: 26085094] (Mouse)
• Gillgrass A, Chew M, Krneta T, Ashkar A Overexpression of IL-15 promotes tumor destruction via NK1.1+ cells in a spontaneous breast cancer model. BMC Cancer, 2015-04-16;15(0):293. 2015-04-16 [PMID: 25879689] (Mouse)
• Schroder J, Moll J, Baran P, Grotzinger J, Scheller J, Floss D Non-canonical interleukin 23 receptor complex assembly: p40 protein recruits interleukin 12 receptor beta1 via site II and induces p19/interleukin 23 receptor interaction via site III. J Biol Chem, 2014-11-04;290(1):359-70. 2014-11-04 [PMID: 25371211] (Mouse)
• Haste L, Hulland K, Bolton S, Yesilkaya H, McKechnie K, Andrew P Development and characterization of a long-term murine model of Streptococcus pneumoniae infection of the lower airways. Infect Immun, 2014-05-27;82(8):3289-98. 2014-05-27 [PMID: 24866797] (Mouse)
• Ribes S, Meister T, Ott M, Redlich S, Janova H, Hanisch U, Nessler S, Nau R Intraperitoneal prophylaxis with CpG oligodeoxynucleotides protects neutropenic mice against intracerebral Escherichia coli K1 infection. J Neuroinflammation, 2014-01-23;11(0):14. 2014-01-23 [PMID: 24456653] (Mouse)
• Berod L, Stuve P, Swallow M, Arnold-Schrauf C, Kruse F, Gentilini M, Freitag J, Holzmann B, Sparwasser T MyD88 signalling in myeloid cells is sufficient to prevent chronic mycobacterial infection. Eur J Immunol, 2014-02-13;44(5):1399-409. 2014-02-13 [PMID: 24435955] (Mouse)
• Serezani CH, Kane S, Collins L Macrophage Dectin-1 Expression Is Controlled by Leukotriene B4 via a GM-CSF/PU.1 Axis. J. Immunol., 2012-06-13;189(2):906-15. 2012-06-13 [PMID: 22696442] (Mouse)
• Gowda NM, Wu X, Gowda DC TLR9 and MyD88 are crucial for the development of protective immunity to malaria. J. Immunol., 2012-04-18;188(10):5073-85. 2012-04-18 [PMID: 22516959] (Mouse)
• Barber DL, Mayer-Barber KD, Feng CG CD4 T cells promote rather than control tuberculosis in the absence of PD-1-mediated inhibition. J. Immunol., 2010-12-20;186(3):1598-607. 2010-12-20 [PMID: 21172867] (Mouse)
• Zhang X, Schmudde I, Laumonnier Y, Pandey MK, Clark JR, Konig P, Gerard NP, Gerard C, Wills-Karp M, Kohl J A critical role for C5L2 in the pathogenesis of experimental allergic asthma. J. Immunol., 2010-10-25;185(11):6741-52. 2010-10-25 [PMID: 20974988] (Mouse)
• Reynolds CM, Draper E, Keogh B, Rahman A, Moloney AP, Mills KH, Loscher CE, Roche HM A conjugated linoleic acid-enriched beef diet attenuates lipopolysaccharide-induced inflammation in mice in part through PPARgamma-mediated suppression of toll-like receptor 4. J. Nutr., 2009-10-21;139(12):2351-7. 2009-10-21 [PMID: 19846417] (Mouse)
• Wan H, Coppens JM, van Helden-Meeuwsen CG, Leenen PJ, Van Rooijen N, Khan NA, Kiekens RC, Benner R, Versnel MA Chorionic gonadotropin alleviates thioglycollate-induced peritonitis by affecting macrophage function. J. Leukoc. Biol., 2009-05-04;86(2):361-70. 2009-05-04 [PMID: 19414540] (Mouse)
• Lembo A, Pelletier M, Iyer R, Timko M, Dudda JC, West TE, Wilson CB, Hajjar AM, Skerrett SJ Administration of a synthetic TLR4 agonist protects mice from pneumonic tularemia. J. Immunol., 2008-06-01;180(11):7574-81. 2008-06-01 [PMID: 18490759] (Mouse)
• Schaefer M, Reiling N, Fessler C, Stephani J, Taniuchi I, Hatam F, Yildirim AO, Fehrenbach H, Walter K, Ruland J, Wagner H, Ehlers S, Sparwasser T Decreased pathology and prolonged survival of human DC-SIGN transgenic mice during mycobacterial infection. J. Immunol., 2008-05-15;180(10):6836-45. 2008-05-15 [PMID: 18453604] (Mouse)
• Hawn TR, Berrington WR, Smith IA, Uematsu S, Akira S, Aderem A, Smith KD, Skerrett SJ Altered inflammatory responses in TLR5-deficient mice infected with Legionella pneumophila. J. Immunol., 2007-11-15;179(10):6981-7. 2007-11-15 [PMID: 17982089] (Mouse)
• Higgins SC, Jarnicki AG, Lavelle EC, Mills KH TLR4 mediates vaccine-induced protective cellular immunity to Bordetella pertussis: role of IL-17-producing T cells. J. Immunol., 2006-12-01;177(11):7980-9. 2006-12-01 [PMID: 17114471] (Mouse)
• Rodriguez N, Wantia N, Fend F, Durr S, Wagner H, Miethke T Differential involvement of TLR2 and TLR4 in host survival during pulmonary infection with Chlamydia pneumoniae. Eur. J. Immunol., 2006-05-01;36(5):1145-55. 2006-05-01 [PMID: 16609927] (Mouse)
• Gilleron M, Nigou J, Nicolle D, Quesniaux V, Puzo G The acylation state of mycobacterial lipomannans modulates innate immunity response through toll-like receptor 2. Chem. Biol., 2006-01-01;13(1):39-47. 2006-01-01 [PMID: 16426970] (Mouse)
• Noulin N, Quesniaux VF, Schnyder-Candrian S, Schnyder B, Maillet I, Robert T, Vargaftig BB, Ryffel B, Couillin I Both hemopoietic and resident cells are required for MyD88-dependent pulmonary inflammatory response to inhaled endotoxin. J. Immunol., 2005-11-15;175(10):6861-9. 2005-11-15 [PMID: 16272344] (Mouse)
• Delale T, Paquin A, Asselin-Paturel C, Dalod M, Brizard G, Bates EE, Kastner P, Chan S, Akira S, Vicari A, Biron CA, Trinchieri G, Briere F MyD88-dependent and -independent murine cytomegalovirus sensing for IFN-alpha release and initiation of immune responses in vivo. J. Immunol., 2005-11-15;175(10):6723-32. 2005-11-15 [PMID: 16272328] (Mouse)
• Fremond CM, Yeremeev V, Nicolle DM, Jacobs M, Quesniaux VF, Ryffel B Fatal Mycobacterium tuberculosis infection despite adaptive immune response in the absence of MyD88. J. Clin. Invest., 2004-12-01;114(12):1790-9. 2004-12-01 [PMID: 15599404] (Mouse)
• Nicolle D, Fremond C, Pichon X, Bouchot A, Maillet I, Ryffel B, Quesniaux VJ Long-term control of Mycobacterium bovis BCG infection in the absence of Toll-like receptors (TLRs): investigation of TLR2-, TLR6-, or TLR2-TLR4-deficient mice. Infect. Immun., 2004-12-01;72(12):6994-7004. 2004-12-01 [PMID: 15557622] (Mouse)
• Zamboni DS, Campos MA, Torrecilhas AC, Kiss K, Samuel JE, Golenbock DT, Lauw FN, Roy CR, Almeida IC, Gazzinelli RT Stimulation of toll-like receptor 2 by Coxiella burnetii is required for macrophage production of pro-inflammatory cytokines and resistance to infection. J. Biol. Chem., 2004-10-13;279(52):54405-15. 2004-10-13 [PMID: 15485838] (Mouse)
• Chong MM, Thomas HE, Kay TW Severe pancreatitis with exocrine destruction and increased islet neogenesis in mice with suppressor of cytokine signaling-1 deficiency. Am. J. Pathol., 2004-09-01;165(3):913-21. 2004-09-01 [PMID: 15331415] (Mouse)
• Matsumoto M, Einhaus D, Gold ES, Aderem A Simvastatin augments lipopolysaccharide-induced proinflammatory responses in macrophages by differential regulation of the c-Fos and c-Jun transcription factors. J. Immunol., 2004-06-15;172(12):7377-84. 2004-06-15 [PMID: 15187114] (Mouse)
• Torres D, Barrier M, Bihl F, Quesniaux VJ, Maillet I, Akira S, Ryffel B, Erard F Toll-like receptor 2 is required for optimal control of Listeria monocytogenes infection. Infect. Immun., 2004-04-01;72(4):2131-9. 2004-04-01 [PMID: 15039335] (Mouse)
• Takahashi M, Kobayashi Y Cytokine production in association with phagocytosis of apoptotic cells by immature dendritic cells. Cell. Immunol., 2003-12-01;226(2):105-15. 2003-12-01 [PMID: 14962498] (Mouse)
• Gilleron M, Quesniaux VF, Puzo G Acylation state of the phosphatidylinositol hexamannosides from Mycobacterium bovis bacillus Calmette Guerin and mycobacterium tuberculosis H37Rv and its implication in Toll-like receptor response. J. Biol. Chem., 2003-05-29;278(32):29880-9. 2003-05-29 [PMID: 12775723] (Mouse)
• O'Keeffe M CpG-DNA aided cross-presentation of soluble antigens by dendritic cells. Eur. J. Immunol., 2002-08-01;32(8):2356-64. 2002-08-01 [PMID: 12209649] (Mouse)
• O'Garra A Reversal of tumor-induced dendritic cell paralysis by CpG immunostimulatory oligonucleotide and anti-interleukin 10 receptor antibody. J. Exp. Med., 2002-08-19;196(4):541-9. 2002-08-19 [PMID: 12186845] (Mouse)

FAQs for IL-12/IL-23 p40 (DY499). (Showing 1 - 1 of 1 FAQs).

1. wondering what the difference is between your quantikine and duo set elisas?
   • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Bioinformatics

• Gene Symbol: Il12b