ATG16L1 Antibody

Images

 
Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HCT116 whole cell extracts. Image from verified customer review.
Immunohistochemistry-Paraffin: ATG16L1 Antibody [NB110-60928] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The ...read more
Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.

Product Details

Summary
Reactivity Hu, Mu, Rt, Bv, Ca, PmSpecies Glossary
Applications WB, EM, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
0.8 mg/ml

ATG16L1 Antibody Summary

Immunogen
A synthetic peptide within residues 1-100 of human ATG16L1 protein. [Swiss-Prot# Q676U5]
Localization
Cytoplasm. Preautophagosomal structure, preautophagosomal structure membrane, peripheral membrane protein. Note: Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
ATG16L1
Purity
Immunogen affinity purified
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 0.5-2 ug/ml
  • Electron Microscopy 1:10-1:500
  • Immunohistochemistry 1:250 - 1:500
  • Immunohistochemistry-Paraffin 1:250 - 1:500
Application Notes
In Western blot analysis, a specific band is seen at ~68kDa. Electron Microscopy and IHC-P applications are reported in scientific literature (PMIDs 22531915 and 25060858 respectively). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Theoretical MW
68 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Control
ATG16L1 Lysate (NBP2-64677)
ATG16L1 Lysate (NBP2-65114)
Control Peptide
ATG16L1 Peptide (NB110-60928PEP)
Reviewed Applications
Read 1 Review rated 4
using
NB110-60928 in the following applications:

Publications
Read Publications using
NB110-60928 in the following applications:

  • IHC
    1 publication
  • 1 publication
  • WB
    2 publications

Reactivity Notes

Human, rat, bovine, canine, mouse and primate.

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
PBS and 30% Glycerol
Preservative
0.1% Sodium Azide
Concentration
0.8 mg/ml
Purity
Immunogen affinity purified

Alternate Names for ATG16L1 Antibody

  • APG16 autophagy 16-like (S. cerevisiae)
  • APG16L beta
  • APG16LFLJ10828
  • APG16-like 1
  • ATG16 autophagy related 16-like (S. cerevisiae)
  • ATG16 autophagy related 16-like 1 (S. cerevisiae)
  • ATG16A
  • ATG16L
  • autophagy-related protein 16-1
  • FLJ00045
  • FLJ10035
  • FLJ22677
  • IBD10
  • WD repeat domain 30
  • WDR30

Background

Atg16L1 (autophagy-related protein 16-1) forms a component of a protein complex that directs LC3 to autophagosomes en route to their fusion with lysosomes, thereby playing a key role in the process of autophagosome maturation. Atg16L1 belongs to WD repeat ATG16 family, exist as homooligomer and interacts with ATG5 as well as RAB33B, and forms a part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kD) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kD). It is found in cytoplasm and gets localized to preautophagosomal structure where it is implicated in the membrane targeting of ATG5. Mice deficient in Atg16L1 (Atg16L1HM) display reduced autophagy and develop, on viral infection, intestinal abnormalities similar to pathologies found in Crohn's disease patients, including abnormalities in the integrity, architecture, and function of Paneth cells, which are specialized secretory epithelial cells of the small intestine. Atg16L1 has also been shown to play a role in modulating proinflammatory responses and population genetic studies have positively linked a common polymorphism in ATG16L1 gene with inflammatory bowel disease type 10 (IBD10).

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NB110-60928
Species: Hu, Mu, Rt, Bv, Ca, Pm
Applications: WB, EM, IHC, IHC-P

Publications for ATG16L1 Antibody (NB110-60928)(7)

We have publications tested in 2 confirmed species: Human, Mouse.

We have publications tested in 3 applications: IHC, IHC-P, WB.


Filter By Application
IHC
(1)
IHC-P
(1)
WB
(2)
All Applications
Filter By Species
Human
(4)
Mouse
(1)
All Species
Showing Publications 1 - 7 of 7.
Publications using NB110-60928 Applications Species
Sun KT, Chen MY, Tu MG et al. MicroRNA-20a regulates autophagy related protein-ATG16L1 in hypoxia-induced osteoclast differentiation. Bone. 2014 Dec 05 [PMID: 25485521] (WB, Mouse)

Details:
ATG16L1 antibody used for WB on RAW264.7 cells subjected to RANKL and M-CSF mediated osteoclast differentiation as well as hypoxic stress (Fig. 1E ). WB also performed on RAW264.7 cells transduced with miR-20a mimics or antagomiR, and Bafiliomycin under hypoxia (Fig. 3B)
WB Mouse
Tang JY, Hsi E, Huang YC et al. Overexpression of Autophagy-Related 16-Like 1 in Patients with Oral Squamous Cell Carcinoma. Pathol. Oncol. Res. 2014 Jul 25 [PMID: 25060858] (IHC-P, Human)

Details:
ATG16L1 antibody used for IHC-P (1:250 dilution) on Human Oral Squamous Cell Carcinoma tissue sections - formalin fixation, 4um paraffin sections, heat induced antigen retrieval with 0.1 M citrate buffer pH 6.0 for 10 min, primary incubation for 30 minutes RT followed by detectionwith REAL Envision Detection System - Peroxidase/DAB. Fig 1 shows cytoplasmic staining / immunoreactivity score (IRS).
IHC-P Human
Juarez E, Carranza C, Hernandez-Sanchez F et al. NOD2 enhances the innate response of alveolar macrophages to Mycobacterium tuberculosis in humans. Eur J Immunol. 2011
Curtis S, Jones CJ, Garrod A et al. Identification of Autophagic Vacuoles and Regulators of Autophagy in Villous Trophoblast from Normal Term Pregnancies and in Fetal Growth Restriction J Matern Fetal Neonatal Med 2012 Oct 8 [PMID: 23039021] (IHC, Human) IHC Human
Tattoli I, Sorbara MT, Vuckovic D et al. Amino Acid starvation induced by invasive bacterial pathogens triggers an innate host defense program Cell Host Microbe 2012 Jun 14 [PMID: 22704617] (WB, Human) WB Human
Juarez E, Carranza C, Hernandez-Sanchez F, Leon-Contreras JC, Hernandez-Pando R, Escobedo D, Torres M, Sada E. NOD2 enhances the innate response of alveolar macrophages to Mycobacterium tuberculosis in humans. Eur J Immunol;42(4):880-9. 2012 Apr. [PMID: 22531915] (Human) Human
Plantinga TS, Crisan TO, Oosting M. Crohn's disease-associated ATG16L1 polymorphism modulates pro-inflammatory cytokine responses selectively upon activation of NOD2. Gut. 2011 Mar 15. [PMID: 21406388]

Review for ATG16L1 Antibody (NB110-60928) (1) 41

Average Rating: 4
(Based on 1 review)
We have 1 review tested in 1 species: Human.

Reviews using NB110-60928:
Filter by Applications
WB
(1)
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Human
(1)
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Images Ratings Applications Species Date Details
Western Blot ATG16L1 NB110-60928
Enlarge
reviewed by:
JIANFENG CHEN
WB Human 04/28/2014
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Summary

ApplicationWestern Blot
Sample TestedHCT116 whole cell lysate
SpeciesHuman
LotB1

Blocking

Blocking DetailsBlocking at room temperature for 1 hr in 5% non-fat milk in TBS-0.05%Tween20

Primary Anitbody

Dilution Ratio1:2000 in 5% non-fat milk in TBS-0.05%Tween20 and incubated at 4 C for overnight

Secondary Antibody

Secondary DescriptionHRP-conjugated goat anti-rabbit IgG
Secondary Manufacturer Cat#Bio-Rad 170-6515
Secondary Concentration1:10000

Details

Detection NotesThermo Scientific ECL substrate (Cat.# 34080), exposure for 10 seconds

Product General Protocols

View specific protocols for ATG16L1 Antibody (NB110-60928): Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

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FAQs for ATG16L1 Antibody (NB110-60928) (0)

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Control Lysate(s)

Secondary Antibodies

 

Isotype Controls

Other Available Formats

Alexa Fluor 405 Labeled NB110-60928AF405
Alexa Fluor 488 Labeled NB110-60928AF488
Alexa Fluor 647 Labeled NB110-60928AF647
Alexa Fluor 700 Labeled NB110-60928AF700
Biotin Labeled NB110-60928B
DyLight 405 Labeled NB110-60928V
DyLight 488 Labeled NB110-60928G
DyLight 550 Labeled NB110-60928R
DyLight 650 Labeled NB110-60928C
FITC Labeled NB110-60928F
HRP Labeled NB110-60928H

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Bioinformatics Tool for ATG16L1 Antibody (NB110-60928)

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Diseases for ATG16L1 Antibody (NB110-60928)

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Blogs on ATG16L1.

ATG16L1 - a key player in the development of the autophagosome
Like apoptosis, autophagy is a highly regulated physiologic process that involves cellular degradation and recycling of organelles and macromolecules. Autophagy is a survival mechanism induced by states of stress, starvation, and infection. A do...  Read full blog post.

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Recent Reviews

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JIANFENG CHEN
04/28/2014
Application: WB
Species: Human

Bioinformatics

Gene Symbol ATG16L1
Entrez
Uniprot