Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HCT116 whole cell extracts. Image from verified customer review.
Immunohistochemistry-Paraffin: ATG16L1 Antibody [NB110-60928] - IHC analysis of formalin fixed paraffin-embedded (FFPE) human colon cancer using ATG16L1 antibody at 1:500 on a Bond Rx autostainer (Leica Biosystems). The ...read more
Western Blot: ATG16L1 Antibody [NB110-60928] - Detection of ATG16L1 using NB110-60928 in HeLa whole cell extracts.
A synthetic peptide within residues 1-100 of human ATG16L1 protein. [Swiss-Prot# Q676U5]
Cytoplasm. Preautophagosomal structure, preautophagosomal structure membrane, peripheral membrane protein. Note: Localized to preautophagosomal structure (PAS) where it is involved in the membrane targeting of ATG5.
Immunogen affinity purified
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In Western blot analysis, a specific band is seen at ~68kDa. Electron Microscopy and IHC-P applications are reported in scientific literature (PMIDs 22531915 and 25060858 respectively). The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
68 kDa. Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
PBS and 30% Glycerol
0.1% Sodium Azide
Immunogen affinity purified
Alternate Names for ATG16L1 Antibody
APG16 autophagy 16-like (S. cerevisiae)
ATG16 autophagy related 16-like (S. cerevisiae)
ATG16 autophagy related 16-like 1 (S. cerevisiae)
autophagy-related protein 16-1
WD repeat domain 30
Atg16L1 (autophagy-related protein 16-1) forms a component of a protein complex that directs LC3 to autophagosomes en route to their fusion with lysosomes, thereby playing a key role in the process of autophagosome maturation. Atg16L1 belongs to WD repeat ATG16 family, exist as homooligomer and interacts with ATG5 as well as RAB33B, and forms a part of either the minor and major complexes respectively composed of 4 sets of ATG12-ATG5 and ATG16L1 (400 kD) or 8 sets of ATG12-ATG5 and ATG16L1 (800 kD). It is found in cytoplasm and gets localized to preautophagosomal structure where it is implicated in the membrane targeting of ATG5. Mice deficient in Atg16L1 (Atg16L1HM) display reduced autophagy and develop, on viral infection, intestinal abnormalities similar to pathologies found in Crohn's disease patients, including abnormalities in the integrity, architecture, and function of Paneth cells, which are specialized secretory epithelial cells of the small intestine. Atg16L1 has also been shown to play a role in modulating proinflammatory responses and population genetic studies have positively linked a common polymorphism in ATG16L1 gene with inflammatory bowel disease type 10 (IBD10).
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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