IL-22 Antibody

• Reactivity: Hu
• Applications: WB
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

IL-22 Antibody Summary

• Description: Purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against stated buffer.
• Immunogen: Prepared from rabbit antiserum after repeated immunizations with a truncated form of recombinant human IL-22 protein from E. coli.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IL22
• Purity: Multi-step

Applications/Dilutions

• Dilutions:
  • Western Blot
• Application Notes: This antibody has been tested in western blotting and suitable for ELISA. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 20 kDa in size corresponding to the human IL-22 protein by western blotting in appropriate cell lysate or extract.

Reactivity Notes

BLAST analysis was used to suggest cross-reactivity with IL-22 from human sources based on 100% homology with the immunizing sequence. Cross-reactivity with IL-22 from other sources has not been determined.

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Preservative: 0.01% Sodium Azide
• Purity: Multi-step

Alternate Names for IL-22 Antibody

• Cytokine Zcyto18
• IL-10-related T-cell-derived inducible factor
• IL22
• IL-22
• IL-D110
• IL-TIF
• ILTIFIL-10-related T-cell-derived-inducible factor
• IL-TIFMGC79382
• interleukin 22
• interleukin-22
• MGC79384
• TIFa
• TIFIL-23
• zcyto18

Background

Interleukin-22 (IL-22 or IL-TIF (IL-10-related T-cell derived inducible factor)) belongs to the IL-10 family of regulatory cytokines which also includes IL-10, IL-19, IL-20, IL-24 and IL-26. IL-22 is produced by T lymphocytes following anti-CD3 stimulation. It inhibits IL-4 production of Th2 cells, activates STAT-1 and STAT-3 in several hepatoma cell lines and upregulates the production of acute phase proteins. It may also be involved in inflammatory responses. Human and mouse IL-22 share 79% amino acid identity. Recombinant human IL-22 is a non-disulfide linked homodimer, signaling through a receptor complex of two subunits, IL-22R and IL-10R-beta, belonging to the class II cytokine receptor family.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Bioinformatics

• Gene Symbol: IL22