IL-22 Antibodies

582-ML) stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Mouse IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_lightbox"> 582-ML) stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Mouse IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582). The ND₅₀ is typically 0.5-2.5 µg/mL." alt="Recombinant Mouse IL-22 (Catalog # 582-ML) stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Mouse IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Mouse IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_thumb" />

582-ML) was serially diluted and captured by Goat Anti-Mouse IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 582-ML) was serially diluted and captured by Goat Anti-Mouse IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

582-ML) was serially diluted and captured by Goat Anti-Mouse IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 582-ML) was serially diluted and captured by Goat Anti-Mouse IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse IL‑22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF582) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

HAF019). A specific band was detected for IL-22 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_lightbox"> HAF019). A specific band was detected for IL-22 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." alt="Western blot shows lysates of human tonsil tissue and human breast cancer tissue. PVDF Membrane was probed with 1 µg/mL of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for IL-22 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1." class="big_thumb" />

NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_lightbox"> NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells." class="big_thumb" />

782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adeno-carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_lightbox"> 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adeno-carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human IL-22 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF782). The ND₅₀ is typically 0.5-2.5 µg/mL." class="big_thumb" />

782-IL) was serially diluted and captured by Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7822) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7821) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 782-IL) was serially diluted and captured by Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7822) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7821) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Recombinant Human IL‑22 (Catalog # 782-IL) was serially diluted and captured by Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7822) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7821) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." alt="PBMC treated with 20ng/mL PMA and 1ug/mL Calcium iono for 2 hours followed by 1ug/mL brefeldin A for 4 hours were stained with Mouse Anti-Human CD4 Fluorescein‑conjugated Monoclonal Antibody (Catalog # FAB3791F) and either (A) Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7821) or (B) isotype control antibody (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # FC005). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adeno-carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-22 Monoclonal Antibody (Catalog # MAB7821). The ND₅₀ is typically 1-5 µg/mL." class="big_lightbox"> 782-IL) stimulates IL-10 secretion in the COLO 205 human colorectal adeno-carcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 secretion elicited by Recombinant Human IL-22 (1 ng/mL) is neutralized (green line) by increasing concentrations of Human IL-22 Monoclonal Antibody (Catalog # MAB7821). The ND₅₀ is typically 1-5 µg/mL." class="big_thumb" />

782-IL) was serially diluted and captured by Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7822) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7821) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox"> 782-IL) was serially diluted and captured by Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7822) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human IL‑22 Monoclonal Antibody (Catalog # MAB7821) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />

MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and (A) Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) or (B) isotype control antibody (MAB006) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules." class="big_lightbox"> MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and (A) Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) or (B) isotype control antibody (MAB006) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules." alt="Mouse splenocytes differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3e monoclonal antibody (MAB484, 5 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (240-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 5 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and (A) Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) or (B) isotype control antibody (MAB006) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules." class="big_thumb" />

DY217B). IL-10 Secretion elicited by Recombinant Mouse IL-22 (500 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB5821). The ND₅₀ is typically 0.5-6 µg/mL." class="big_lightbox"> DY217B). IL-10 Secretion elicited by Recombinant Mouse IL-22 (500 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB5821). The ND₅₀ is typically 0.5-6 µg/mL." alt="Recombinant Mouse IL-22 stimulates IL-10 secretion in the COLO 205 human colorectal adenocarcinoma cell line in a dose-dependent manner (orange line), as measured by the Human IL-10 DuoSet ELISA Development Kit (Catalog # DY217B). IL-10 Secretion elicited by Recombinant Mouse IL-22 (500 pg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB5821). The ND₅₀ is typically 0.5-6 µg/mL." class="big_thumb" />

MAB484, 10 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (100-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 6 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, or (B) resting, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). Quadrant markers were set based on isotype control antibody (MAB006). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules. " class="big_lightbox"> MAB484, 10 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (100-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 6 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, or (B) resting, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). Quadrant markers were set based on isotype control antibody (MAB006). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules. " alt="Mouse splenocytes (A) differentiated to Th17 cells with plate-bound Rat anti-Mouse CD3e monoclonal antibody (MAB484, 10 µg/mL) plus Goat anti-Mouse CD28 (AF483, 5 µg/mL), Recombinant Human TGF-beta 1 (100-B, 10 ng/mL) Recombinant Mouse IL-23 (1887-ML, 20 ng/mL), Recombinant Mouse IL-6 (406-ML, 40 ng/mL), Recombinant Mouse IL-1 beta (401-ML, 10 ng/mL), and Rat anti-Mouse IFN-gamma (MAB485, 10 µg/mL) for 6 days then re-stimulated with PMA (50 ng/ml), Ca2+ Ionomycin (200 ng/ml) and Brefeldin A ( 5 μg/ml) for 4 hours, or (B) resting, were stained with APC-conjugated Rat anti-Mouse CD4 Monoclonal Antibody (FAB554A) and Rat Anti-Mouse IL-22 Monoclonal Antibody (Catalog # MAB582) followed by PE-conjugated Goat anti-Rat secondary antibody (F0105B). Quadrant markers were set based on isotype control antibody (MAB006). To facilitate intracellular staining, cells were fixed and permeabilized with FlowX FoxP3/Transcription Factor Fixation & Perm Buffer Kit. (FC012). View our protocol for Staining Intracellular Molecules. " class="big_thumb" />