Recombinant Human IL-15 Protein


1 µg/lane of Recombinant Human IL-15 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a major band at 10 kDa.
Recombinant Human IL-15 (Catalog #247-ILB) stimulates cell proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.3-2.6 ng/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-15 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.3-2.6 ng/mL.
The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554).
E. coli-derived human IL-15 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
10 kDa, reducing conditions
Read Publications using
247-ILB in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-15 Protein

  • IL15
  • IL-15
  • IL-15MGC9721
  • interleukin 15
  • interleukin-15


Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1, 2). Mature human IL-15 protein shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of Interleukin 15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (3). The IL-15 protein binds with high affinity to IL-15 R alpha (4). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (5). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (7). This enables cells to respond to Interleukin 15 even if they do not express IL-15 R alpha (6). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 protein in circulation (8-10). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (4, 9). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (11). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells (12 - 16). An important component of these functions is the ability of IL-15 to induce dendritic cell differentiation and inflammatory activation (11, 14). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8+ T cells (17). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (18).

  1. De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  2. Grabstein, K. et al. (1994) Science 264:965.
  3. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  4. Giri, J.G. et al. (1995) EMBO J. 14:3654.
  5. Giri, J. et al. (1994) EMBO J. 13:2822.
  6. Dubois, S. et al. (2002) Immunity 17:537.
  7. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479.
  8. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  9. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  10. Bergamaschi, C. et al. (2012) Blood 120:e1.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  12. Mortier, E. et al. (2003) J. Exp. Med. 205:1213.
  13. Gordy, L.E. et al. (2011) J. Immunol. 187:6335.
  14. Harris, K.M. (2011) J. Leukoc. Biol. 90:727.
  15. Xia, J. et al. (2010) Clin. Immunol. 134:130.
  16. Schluns, K.S. et al. (2002) J. Immunol. 168:4827.
  17. Davies, E. et al. (2010) J. Leukoc. Biol. 88:529.
  18. Barra, N.G. et al. (2010) Obesity 18:1601.

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Publications for IL-15 (247-ILB)(135)

We have publications tested in 7 confirmed species: Human, Mouse, Bovine, CD45.1 P14TCM, N/A, Primate - Macaca mulatta (Rhesus Macaque), Xenograft.

We have publications tested in 6 applications: Bioassay, CAR-T (Bioassay), Cell Culture, ELISA (Standard), In Vivo, Western Blot.

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CD45.1 P14TCM
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 135. Show All 135 Publications.
Publications using 247-ILB Applications Species
N Gimenez, R Tripathi, A Giró, L Rosich, M López-Guer, I López-Orej, H Playa-Albi, F Arenas, JM Mas, P Pérez-Galá, J Delgado, E Campo, J Farrés, D Colomer Systems biology drug screening identifies statins as enhancers of current therapies in chronic lymphocytic leukemia Scientific Reports, 2020;10(1):22153. 2020 [PMID: 33335123] (Cell Culture, Human) Cell Culture Human
J Aram, N Frakich, E Morandi, M Alrouji, A Samaraweer, D Onion, I Spendlove, SL Colombo, R Tanasescu, B Gran, CS Constantin Increased IL-2 and Reduced TGF-&amp;beta Upon T-Cell Stimulation are Associated with GM-CSF Upregulation in Multiple Immune Cell Types in Multiple Sclerosis Biomedicines, 2020;8(7):. 2020 [PMID: 32708498] (Cell Culture, Human) Cell Culture Human
SJ Schober, M Thiede, H Gassmann, C Prexler, B Xue, D Schirmer, D Wohlleber, S Stein, TGP Grünewald, DH Busch, GHS Richter, SEG Burdach, U Thiel MHC Class I-Restricted TCR-Transgenic CD4+ T Cells Against STEAP1 Mediate Local Tumor Control of Ewing Sarcoma In Vivo Cells, 2020;9(7):. 2020 [PMID: 32610710] (Cell Culture, Human) Cell Culture Human
SR Morris, B Chen, JC Mudd, S Panigrahi, CL Shive, SF Sieg, CM Cameron, DA Zidar, NT Funderburg, SA Younes, B Rodriguez, S Gianella, MM Lederman, ML Freeman &quot;Inflammescent&quot; CX3CR1+CD57+ CD8 T cells are generated and expanded by IL-15 JCI Insight, 2020;0(0):. 2020 [PMID: 32369455] (Bioassay, Human) Bioassay Human
R Fonseca, LK Beura, CF Quarnstrom, HE Ghoneim, Y Fan, CC Zebley, MC Scott, NJ Fares-Fred, S Wijeyesing, EA Thompson, H Borges da, V Vezys, B Youngblood, D Masopust Developmental plasticity allows outside-in immune responses by resident memory T cells Nat. Immunol., 2020;0(0):. 2020 [PMID: 32066954] (Cell Culture, CD45.1 P14TCM) Cell Culture CD45.1 P14TCM
JB McBrien, M Mavigner, L Franchitti, SA Smith, E White, GK Tharp, H Walum, K Busman-Suh, CR Aguilera-S, WO Thayer, RA Spagnuolo, M Kovarova, A Wahl, B Cervasi, DM Margolis, TH Vanderford, DG Carnathan, M Paiardini, JD Lifson, JH Lee, JT Safrit, SE Bosinger, JD Estes, CA Derdeyn, JV Garcia, DA Kulpa, A Chahroudi, G Silvestri Robust and persistent reactivation of SIV and HIV by N-803 and depletion of CD8+ cells Nature, 2020;0(0):. 2020 [PMID: 31969705] (Cell Culture, Mouse) Cell Culture Mouse
L Swadling, LJ Pallett, MO Diniz, JM Baker, OE Amin, KA Stegmann, AR Burton, NM Schmidt, A Jeffery-Sm, N Zakeri, K Suveizdyte, F Froghi, G Fusai, WM Rosenberg, BR Davidson, A Schurich, AK Simon, MK Maini Human Liver Memory CD8+ T Cells Use Autophagy for Tissue Residence Cell Rep, 2020;30(3):687-698.e6. 2020 [PMID: 31968246] (Cell Culture, Human) Cell Culture Human
DN Nguyen, TL Roth, PJ Li, PA Chen, R Apathy, MR Mamedov, LT Vo, VR Tobin, D Goodman, E Shifrut, JA Bluestone, JM Puck, FC Szoka, A Marson Polymer-stabilized Cas9 nanoparticles and modified repair templates increase genome editing efficiency Nat. Biotechnol., 2019;0(0):. 2019 [PMID: 31819258] (Cell Culture, Human) Cell Culture Human
JD Ventura, J Beloor, E Allen, T Zhang, KA Haugh, PD Uchil, C Ochsenbaue, C Kieffer, P Kumar, TJ Hope, W Mothes Longitudinal bioluminescent imaging of HIV-1 infection during antiretroviral therapy and treatment interruption in humanized mice PLoS Pathog., 2019;15(12):e1008161. 2019 [PMID: 31805155] (Cell Culture, Human) Cell Culture Human
ZY Li, JJ Zhou, CL Luo, LM Zhang Activation of TGR5 alleviates inflammation in rheumatoid arthritis peripheral blood mononuclear cells and in mice with collagen II?induced arthritis Mol Med Rep, 2019;20(5):4540-4550. 2019 [PMID: 31702035] (Cell Culture, Human) Cell Culture Human
Show All 135 Publications.

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Gene Symbol IL15