Recombinant Human IL-15 Protein

Images

 
1 µg/lane of Recombinant Human IL-15 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a major band at 10 kDa.
Recombinant Human IL-15 (Catalog #247-ILB) stimulates cell proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.3-2.6 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-15 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.3-2.6 ng/mL.
The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554).
Source
E. coli-derived human IL-15 protein
Asn49-Ser162
Accession #
N-terminal Sequence
Asn49
Protein/Peptide Type
Recombinant Proteins
Gene
IL15
Purity
>97%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
10 kDa, reducing conditions
Publications
Read Publications using
247-ILB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-15 Protein

  • IL15
  • IL-15
  • IL-15MGC9721
  • interleukin 15
  • interleukin-15

Background

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1, 2). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (3). IL-15 binds with high affinity to IL-15 R alpha (4). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (5). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (7). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (6). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation (8-10). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (4, 9). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (11). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells (12 - 16). An important component of these functions is the ability of
IL-15 to induce dendritic cell differentiation and inflammatory activation (11, 14). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8+ T cells (17). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (18).
  1. De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  2. Grabstein, K. et al. (1994) Science 264:965.
  3. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  4. Giri, J.G. et al. (1995) EMBO J. 14:3654.
  5. Giri, J. et al. (1994) EMBO J. 13:2822.
  6. Dubois, S. et al. (2002) Immunity 17:537.
  7. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479.
  8. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  9. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  10. Bergamaschi, C. et al. (2012) Blood 120:e1.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  12. Mortier, E. et al. (2003) J. Exp. Med. 205:1213.
  13. Gordy, L.E. et al. (2011) J. Immunol. 187:6335.
  14. Harris, K.M. (2011) J. Leukoc. Biol. 90:727.
  15. Xia, J. et al. (2010) Clin. Immunol. 134:130.
  16. Schluns, K.S. et al. (2002) J. Immunol. 168:4827.
  17. Davies, E. et al. (2010) J. Leukoc. Biol. 88:529.
  18. Barra, N.G. et al. (2010) Obesity 18:1601.

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247-ILB
Species: Hu
Applications: Bioactivity

Publications for IL-15 (247-ILB)(103)

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 5 applications: Bioassay, Cell Culture Substrate, ELISA Standard, In Vivo, WB Ctrl.


Filter By Application
Bioassay
(94)
Cell Culture Substrate
(2)
ELISA Standard
(3)
In Vivo
(3)
WB Ctrl
(1)
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Human
(89)
Mouse
(7)
Bovine
(1)
N/A
(4)
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 103. Show All 103 Publications.
Publications using 247-ILB Applications Species
R Liu, J Merola, TD Manes, L Qin, GT Tietjen, F López-Girá, V Broecker, C Fang, C Xie, PM Chen, NC Kirkiles-S, D Jane-Wit, JS Pober Interferon-? converts human microvascular pericytes into negative regulators of alloimmunity through induction of indoleamine 2,3-dioxygenase 1 JCI Insight, 2018;3(5):. 2018 [PMID: 29515027] (Bioassay, Human) Bioassay Human
T Oth, THPM Habets, WTV Germeraad, MI Zonneveld, GMJ Bos, J Vanderloch Pathogen recognition by NK cells amplifies the pro-inflammatory cytokine production of monocyte-derived DC via IFN-? BMC Immunol., 2018;19(1):8. 2018 [PMID: 29433450] (Bioassay, Human) Bioassay Human
M Felices, AJ Lenvik, R McElmurry, S Chu, P Hinderlie, L Bendzick, MA Geller, J Tolar, BR Blazar, JS Miller Continuous treatment with IL-15 exhausts human NK cells via a metabolic defect JCI Insight, 2018;3(3):. 2018 [PMID: 29415897] (Bioassay, Human) Bioassay Human
AH Turaj, KL Cox, CA Penfold, RR French, CI Mockridge, JE Willoughby, AL Tutt, J Griffiths, PWM Johnson, MJ Glennie, R Levy, MS Cragg, SH Lim Augmentation of CD134 (OX40)-dependent NK anti-tumour activity is dependent on antibody cross-linking Sci Rep, 2018;8(1):2278. 2018 [PMID: 29396470] (Bioassay, Human) Bioassay Human
C Gavegnano, JH Brehm, FP Dupuy, A Talla, SP Ribeiro, DA Kulpa, C Cameron, S Santos, SJ Hurwitz, VC Marconi, JP Routy, L Sabbagh, RF Schinazi, RP Sékaly Novel mechanisms to inhibit HIV reservoir seeding using Jak inhibitors PLoS Pathog., 2017;13(12):e1006740. 2017 [PMID: 29267399] (Bioassay, Human) Bioassay Human
E Kuca-Warna, W Kurowska, M Prochorec-, A Radzikowsk, T Burakowski, U Skalska, M Massalska, M Pleba?czyk, B Ma?dyk-Now, I S?owi?ska, R Gasik, W Ma?li?ski Rheumatoid arthritis bone marrow environment supports Th17 response Arthritis Res. Ther., 2017;19(1):274. 2017 [PMID: 29216915] (Bioassay, Human) Bioassay Human
JX Lin, N Du, P Li, M Kazemian, T Gebregiorg, R Spolski, WJ Leonard Critical functions for STAT5 tetramers in the maturation and survival of natural killer cells Nat Commun, 2017;8(1):1320. 2017 [PMID: 29105654] (Bioassay, Mouse) Bioassay Mouse
ML Clénet, F Gagnon, AC Moratalla, EC Viel, N Arbour Peripheral human CD4(+)CD8(+) T lymphocytes exhibit a memory phenotype and enhanced responses to IL-2, IL-7 and IL-15 Sci Rep, 2017;7(1):11612. 2017 [PMID: 28912605] (Bioassay, Human) Bioassay Human
W Li, Y Wu, D Kong, H Yang, Y Wang, J Shao, Y Feng, W Chen, L Ma, T Ying, DS Dimitrov One-domain CD4 Fused to Human Anti-CD16 Antibody Domain Mediates Effective Killing of HIV-1-Infected Cells Sci Rep, 2017;7(1):9130. 2017 [PMID: 28831040] (Bioassay, Human) Bioassay Human
CA Ramos, B Ballard, H Zhang, O Dakhova, AP Gee, Z Mei, M Bilgi, MF Wu, H Liu, B Grilley, CM Bollard, BH Chang, CM Rooney, MK Brenner, HE Heslop, G Dotti, B Savoldo Clinical and immunological responses after CD30-specific chimeric antigen receptor-redirected lymphocytes J. Clin. Invest., 2017;0(0):. 2017 [PMID: 28805662] (Bioassay, Human) Bioassay Human
Show All 103 Publications.

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Bioinformatics

Gene Symbol IL15
Entrez
Uniprot