Recombinant Human IL-15 Protein

Images

 
1 µg/lane of Recombinant Human IL-15 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a major band at 10 kDa.
Recombinant Human IL-15 (Catalog #247-ILB) stimulates cell proliferation in the MO7e human megakaryocytic leukemic cell line. The ED50 for this effect is 0.3-2.6 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-15 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is 0.3-2.6 ng/mL.
The specific activity of Recombinant Human IL-15 is approximately 4.5 x 105 U/μg, which is calibrated against recombinant human IL-15 WHO International Standard (NIBSC code: 95/554).
Source
E. coli-derived human IL-15 protein
Asn49-Ser162
Accession #
N-terminal Sequence
Asn49
Protein/Peptide Type
Recombinant Proteins
Gene
IL15
Purity
>97%, by SDS-PAGE with silver staining.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Theoretical MW
13 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
SDS-PAGE
10 kDa, reducing conditions
Publications
Read Publications using
247-ILB in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE with silver staining.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-15 Protein

  • IL15
  • IL-15
  • IL-15MGC9721
  • interleukin 15
  • interleukin-15

Background

Interleukin 15 (IL-15) is a widely expressed 14 kDa cytokine that is structurally and functionally related to IL-2 and plays an important role in many immunological diseases (1, 2). Mature human IL-15 shares 70% amino acid sequence identity with mouse and rat IL-15. Alternative splicing generates isoforms of IL-15 with either a long or short signal peptide (LSP or SSP), and the SSP isoform is retained intracellularly (3). IL-15 binds with high affinity to IL-15 R alpha (4). It binds with lower affinity to a complex of IL-2 R beta and the common gamma chain ( gamma c) which are also subunits of the IL-2 receptor complex (5). IL-15 associates with IL-15 R alpha in the endoplasmic reticulum, and this complex is expressed on the cell surface (6). The dominant mechanism of IL-15 action is known as transpresentation in which IL-15 and IL-15 R alpha are coordinately expressed on the surface of one cell and interact with complexes of IL-2 R beta / gamma c on adjacent cells (7). This enables cells to respond to IL-15 even if they do not express IL-15 R alpha (6). In human and mouse, soluble IL-15-binding forms of IL-15 R alpha can be generated by proteolytic shedding and bind up nearly all the IL-15 in circulation (8-10). Soluble IL-15 R alpha functions as an inhibitor that limits IL-15 action (4, 9). Ligation of membrane-associated IL-15/IL-15 R alpha complexes also induces reverse signaling that promotes activation of the IL-15/IL-15 R alpha expressing cells (11). IL-15 induces or enhances the differentiation, maintenance, or activation of multiple T cell subsets including NK, NKT, Th17, Treg, and CD8+ memory cells (12 - 16). An important component of these functions is the ability of
IL-15 to induce dendritic cell differentiation and inflammatory activation (11, 14). IL-15 exhibits anti-tumor activity independent of its actions on NK cells or CD8+ T cells (17). It also inhibits the deposition of lipid in adipocytes, and its circulating levels are decreased in obesity (18).
  1. De Sabatino, A. et al. (2011) Cytokine Growth Factor Rev. 22:19.
  2. Grabstein, K. et al. (1994) Science 264:965.
  3. Tagaya, Y. et al. (1997) Proc. Natl. Acad. Sci. USA 94:14444.
  4. Giri, J.G. et al. (1995) EMBO J. 14:3654.
  5. Giri, J. et al. (1994) EMBO J. 13:2822.
  6. Dubois, S. et al. (2002) Immunity 17:537.
  7. Castillo, E.F. and K.S. Schluns (2012) Cytokine 59:479.
  8. Budagian, V. et al. (2004) J. Biol. Chem. 279:40368.
  9. Mortier, E. et al. (2004) J. Immunol. 173:1681.
  10. Bergamaschi, C. et al. (2012) Blood 120:e1.
  11. Budagian, V. et al. (2004) J. Biol. Chem. 279:42192.
  12. Mortier, E. et al. (2003) J. Exp. Med. 205:1213.
  13. Gordy, L.E. et al. (2011) J. Immunol. 187:6335.
  14. Harris, K.M. (2011) J. Leukoc. Biol. 90:727.
  15. Xia, J. et al. (2010) Clin. Immunol. 134:130.
  16. Schluns, K.S. et al. (2002) J. Immunol. 168:4827.
  17. Davies, E. et al. (2010) J. Leukoc. Biol. 88:529.
  18. Barra, N.G. et al. (2010) Obesity 18:1601.

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Publications for IL-15 (247-ILB)(74)

We have publications tested in 5 confirmed species: Human, Mouse, Bovine, N/A, Primate - Macaca mulatta (Rhesus Macaque).

We have publications tested in 5 applications: Bioassay, Cell Culture Substrate, ELISA Standard, In Vivo, WB Ctrl.


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Bioassay
(66)
Cell Culture Substrate
(2)
ELISA Standard
(3)
In Vivo
(2)
WB Ctrl
(1)
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Human
(62)
Mouse
(5)
Bovine
(1)
N/A
(4)
Primate - Macaca mulatta (Rhesus Macaque)
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Showing Publications 1 - 10 of 74. Show All 74 Publications.
Publications using 247-ILB Applications Species
SR Kim, JM Lewis, BM Cyrenne, PF Monico, FN Mirza, KR Carlson, FM Foss, M Girardi BET inhibition in advanced cutaneous T cell lymphoma is synergistically potentiated by BCL2 inhibition or HDAC inhibition Oncotarget, 2018;9(49):29193-29207. 2018 [PMID: 30018745] (Bioassay, Human) Bioassay Human
X Zhao, S Sankaran, J Yap, CT Too, ZZ Ho, G Dolton, M Legut, EC Ren, AK Sewell, A Bertoletti, PA MacAry, J Brzostek, NRJ Gascoigne Nonstimulatory peptide-MHC enhances human T-cell antigen-specific responses by amplifying proximal TCR signaling Nat Commun, 2018;9(1):2716. 2018 [PMID: 30006605] (Bioassay, Human) Bioassay Human
Mongini P, Gupta R, Boyle E, Nieto J, Lee H, Stein J, Bandovic J, Stankovic T, Barrientos J, Kolitz J, Allen S, Rai K, Chu C, Chiorazzi N TLR-9 and IL-15 Synergy Promotes the In Vitro Clonal Expansion of Chronic Lymphocytic Leukemia B Cells. J Immunol, 2015;195(3):901-23. 2015 [PMID: 26136429] (Bioassay, Human) Bioassay Human
Yokota S, Yoshida O, Dou L, Spadaro A, Isse K, Ross M, Stolz D, Kimura S, Du Q, Demetris A, Thomson A, Geller D IRF-1 promotes liver transplant ischemia/reperfusion injury via hepatocyte IL-15/IL-15Ralpha production. J Immunol, 2015;194(12):6045-56. 2015 [PMID: 25964490] (In Vivo, Mouse) In Vivo Mouse
Sharei A, Trifonova R, Jhunjhunwala S, Hartoularos G, Eyerman A, Lytton-Jean A, Angin M, Sharma S, Poceviciute R, Mao S, Heimann M, Liu S, Talkar T, Khan O, Addo M, von Andrian U, Anderson D, Langer R, Lieberman J, Jensen K Ex vivo cytosolic delivery of functional macromolecules to immune cells. PLoS ONE, 2015;10(4):e0118803. 2015 [PMID: 25875117] (Bioassay, Human) Bioassay Human
Broux B, Mizee M, Vanheusden M, van der Pol S, van Horssen J, Van Wijmeersch B, Somers V, de Vries H, Stinissen P, Hellings N IL-15 amplifies the pathogenic properties of CD4+CD28- T cells in multiple sclerosis. J Immunol, 2015;194(5):2099-109. 2015 [PMID: 25617471] (Bioassay, Human) Bioassay Human
Li H, Richert-Spuhler L, Evans T, Gillis J, Connole M, Estes J, Keele B, Klatt N, Reeves R Hypercytotoxicity and rapid loss of NKp44+ innate lymphoid cells during acute SIV infection. PLoS Pathog, 2014;10(12):e1004551. 2014 [PMID: 25503264] (Bioassay, Primate - Macaca mulatta (Rhesus Macaque)) Bioassay Primate - Macaca mulatta (Rhesus Macaque)
Arber C, Feng X, Abhyankar H, Romero E, Wu M, Heslop H, Barth P, Dotti G, Savoldo B Survivin-specific T cell receptor targets tumor but not T cells. J Clin Invest, 2015;125(1):157-68. 2015 [PMID: 25415440] (Bioassay, Human) Bioassay Human
Nair S, Fang M, Sigal L The natural killer cell dysfunction of aged mice is due to the bone marrow stroma and is not restored by IL-15/IL-15Ralpha treatment. Aging Cell, 2015;14(2):180-90. 2015 [PMID: 25399821] (In Vivo, Mouse) In Vivo Mouse
Lam, Sharon, Sung, Julia, Cruz, Conrad, Castillo-Caro, Paul, Ngo, Minhtran, Garrido, Carolina, Kuruc, Joann, Archin, Nancie, Rooney, Cliona, Margolis, David, Bollard, Catherin Broadly-specific cytotoxic T cells targeting multiple HIV antigens are expanded from HIV+ patients: implications for immunotherapy. Mol Ther, 2015;23(2):387-95. 2015 [PMID: 25366030] (Bioassay, Human) Bioassay Human
Show All 74 Publications.

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Bioinformatics

Gene Symbol IL15
Uniprot