Recombinant Human IL-7 Protein

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Recombinant Human IL-7 (207-IL) stimulates proliferation of PHA-activated human peripheral blood lymphocytes in a dose-dependent manner. The ED50 for this effect is 0.1-0.5 ng/mL.

Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-7 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.1-0.5 ng/mL.
The specific activity of Recombinant Human IL-7 is approximately 4.4 x 105 IU/μg, which is calibrated against human IL-7 WHO International Standard (NIBSC code: 90/530).   Specific activity is for reference purposes only and is not routinely tested.  
Source
E. coli-derived human IL-7 protein
Asp26-His177, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL7
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
207-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein. *1 mg pack size (01M) is supplied as a 0.2 µm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-7 Protein

  • IL7
  • IL-7
  • IL-7interleukin-7
  • interleukin 7
  • Lymphopoietin-1
  • PBGF

Background

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1‑4). Human IL‑7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide (3). Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, porcine, feline and canine IL-7. Human and mouse IL-7 exhibit cross-species activity (2, 3). IL-7 protein is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL-7 protein is limiting in healthy animals, but increases during lymphopenia (1, 6). IL-7 signals through a complex of the IL-7 Receptor alpha subunit (IL-7 R alpha , also known as CD127) with the common gamma chain ( gamma c) (1). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21 (1). IL-7 R alpha is expressed on double negative (CD4-CD8-) and CD4+ or CD8+ single positive naïve and memory T cells, but undergoes IL-7-mediated down‑regulation and shedding during antigen-driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6-11). IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2 (9-11). It is required for optimal T cell-dendritic cell interaction (6). IL-7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL-7 functions in both mouse and human pro-B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).

  1. Sasson, S.C. et al. (2006) Curr. Drug Targets 7:1571.
  2. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  3. Goodwin, R.G. et al. (1990) Proc. Natl. Acad. Sci. USA 86:302.
  4. Namen, A.E. et al. (1988) Nature 333:571.
  5. Shalapour, S. et al. (2012) PLoS ONE 7: e31939.
  6. Saini, M. et al. (2009) Blood 113:5793.
  7. Park, J.H. et al. (2004) Immunity 21:289.
  8. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  9. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
  10. Seddon, B. et al. (2003) Nat. Immunol. 4:680.
  11. Schluns, K.S. et al. (2000) Nat. Immunol. 5:426.
  12. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  13. Pribyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
  14. Johnson, K. et al. (2012) J. Immunol. 188:6084.
  15. Nodland, S.E. et al. (2011) Blood 118:2116.

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Publications for IL-7 (207-IL)(139)

We have publications tested in 6 confirmed species: Human, Mouse, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon).

We have publications tested in 6 applications: Bioassay, CAR-T (Bioassay), CAR-T (Differentiation), Cell Culture, ELISA (Standard), In Vivo.


Filter By Application
Bioassay
(109)
CAR-T (Bioassay)
(3)
CAR-T (Differentiation)
(1)
Cell Culture
(15)
ELISA (Standard)
(4)
In Vivo
(1)
All Applications
Filter By Species
Human
(126)
Mouse
(4)
N/A
(3)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Primate - Papio cynocephalus (Yellow Baboon)
(1)
All Species
Showing Publications 1 - 10 of 139. Show All 139 Publications.
Publications using 207-IL Applications Species
J Boustani, ELM Joseph, E Martin, S Benhmida, B Lecoester, F Tochet, C Mirjolet, C Chevalier, D Thibouw, N Vulquin, S Servagi, X Sun, O Adotévi Cisplatin-based chemoradiation decreases telomerase-specific CD4 TH1 response but increases immune suppressive cells in peripheral blood BMC immunology, 2021;22(1):38. 2021 [PMID: 34144673] (Bioassay, Human) Bioassay Human
F Nicoli, S Pacifico, E Gallerani, E Marzola, V Albanese, V Finessi, S Llewellyn-, DA Price, V Appay, P Marconi, R Guerrini, A Caputo, R Gavioli Use of a Novel Peptide Welding Technology Platform for the Development of B- and T-Cell Epitope-Based Vaccines Vaccines, 2021;9(5):. 2021 [PMID: 34069535] (Bioassay, Human) Bioassay Human
S Jung, E Appleton, M Ali, GM Church, A Del Sol A computer-guided design tool to increase the efficiency of cellular conversions Nature Communications, 2021;12(1):1659. 2021 [PMID: 33712564] (Bioassay, Human) Bioassay Human
E Kidzeru, MA Gasper, D Shao, PT Edlefsen, N Lejarcegui, E Havyariman, K Urdahl, S Gantt, H Horton, H Jaspan, A Gervassi Myeloid-derived suppressor cells and their association with vaccine immunogenicity in South African infants Journal of leukocyte biology, 2021;0(0):. 2021 [PMID: 33477200] (Bioassay, Human) Bioassay Human
S Nagel, C Pommerenke, C Meyer, RAF MacLeod, HG Drexler Aberrant expression of NKL homeobox genes HMX2 and HMX3 interferes with cell differentiation in acute myeloid leukemia PLoS ONE, 2020;15(10):e0240120. 2020 [PMID: 33048949] (Cell Culture, Human) Cell Culture Human
M Matsushita, S Saito, S Yokoe, D Ichikawa, Y Hattori Characteristics of a Novel Target Antigen Against Myeloma Cells for Immunotherapy Vaccines (Basel), 2020;8(4):. 2020 [PMID: 33023190] (Bioassay, Human) Bioassay Human
CR Maldini, K Gayout, RS Leibman, DL Dopkin, JP Mills, X Shan, JA Glover, JL Riley HIV-Resistant and HIV-Specific CAR-Modified CD4+ T Cells Mitigate HIV Disease Progression and Confer CD4+ T Cell Help In�Vivo Mol. Ther., 2020;0(0):. 2020 [PMID: 32454027] (CAR-T (Differentiation), Human) CAR-T (Differentiation) Human
F Hsiao, J Frouard, A Gramatica, G Xie, S Telwatte, GQ Lee, P Roychoudhu, R Schwarzer, X Luo, SA Yukl, S Lee, R Hoh, SG Deeks, RB Jones, M Cavrois, WC Greene, NR Roan Tissue memory CD4+ T cells expressing IL-7 receptor-alpha (CD127) preferentially support latent HIV-1 infection PLoS Pathog., 2020;16(4):e1008450. 2020 [PMID: 32353080] (Cell Culture, Human) Cell Culture Human
A Mitra, MC Andrews, W Roh, MP De Macedo, CW Hudgens, F Carapeto, S Singh, A Reuben, F Wang, X Mao, X Song, K Wani, S Tippen, KS Ng, A Schalck, DA Sakellario, E Chen, SM Reddy, CN Spencer, D Wiesnoski, LD Little, C Gumbs, ZA Cooper, EM Burton, P Hwu, MA Davies, J Zhang, C Bernatchez, N Navin, P Sharma, JP Allison, JA Wargo, C Yee, MT Tetzlaff, WJ Hwu, AJ Lazar, PA Futreal Spatially resolved analyses link genomic and immune diversity and reveal unfavorable neutrophil activation in melanoma Nat Commun, 2020;11(1):1839. 2020 [PMID: 32296058] (Cell Culture, Human) Cell Culture Human
DM Tufa, T Shank, AM Yingst, GD Trahan, S Shim, J Lake, R Woods, K Jones, MR Verneris Prolactin Acts on Myeloid Progenitors to Modulate SMAD7 Expression and Enhance Hematopoietic Stem Cell Differentiation into the NK Cell Lineage Sci Rep, 2020;10(1):6335. 2020 [PMID: 32286456] (Bioassay, Human) Bioassay Human
Show All 139 Publications.

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Bioinformatics

Gene Symbol IL7
Uniprot