Recombinant Human IL-7 Protein

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Product Details

Summary
Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

Recombinant Human IL-7 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). Yokota, T. et al. (1986) Proc. Natl. Acad. Sci. USA 83:5894. The ED50 for this effect is 0.1-0.5 ng/mL.
Source
E. coli-derived human IL-7 protein
Asp26-His177, with an N-terminal Met
Accession #
N-terminal Sequence
Met
Protein/Peptide Type
Recombinant Proteins
Gene
IL7
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.01 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
207-IL in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 50 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-7 Protein

  • IL7
  • IL-7
  • IL-7interleukin-7
  • interleukin 7
  • Lymphopoietin-1
  • PBGF

Background

IL-7 (interleukin-7) is a 25 kDa cytokine of the hemopoietin family that plays important roles in lymphocyte differentiation, proliferation, and survival (1-4). Human IL‑7 cDNA encodes 177 amino acids (aa) that include a 25 aa signal peptide (3). Human IL-7 shares approximately 60-63% aa sequence identity with mouse, rat, canine and feline IL-7, and 72-76% with equine, bovine, ovine, and porcine IL-7. Human and mouse IL-7 exhibit cross-species activity (2, 3).

IL-7 is produced by a wide variety of cells in primary and secondary lymphoid tissues, including stromal epithelial cells of the thymus, bone marrow, and intestines (1, 2, 5). Circulating IL-7 is limiting in healthy animals, but increases during lymphopenia (1, 6). IL-7 signals through a complex of the IL-7 Receptor alpha subunit (IL-7 R alpha , also known as CD127) with the common gamma chain ( gamma c) (1). The gamma c is also a subunit of the receptors for IL-2, -4, -9, -15, and -21 (1).

IL-7 R alpha is expressed on double negative (CD4-CD8-) and single positive (CD4+ or CD8+) naïve and memory T cells, but undergoes IL-7-mediated down‑regulation and shedding during antigen-driven T cell proliferation, and is absent on regulatory T cells (1, 2, 6-11). IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2 (9-11). It is required for optimal T cell-dendritic cell interaction (6). IL-7 is expressed early in B cell development prior to the appearance of surface IgM (1, 5, 9). In mouse, IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development, while in humans, it is required for T cell but not for B cell development (4, 9, 12, 13). However, IL-7 functions in both mouse and human pro-B cells to suppress premature Ig light chain recombination during proliferative growth (14, 15).

Like other common gamma-chain cytokines like IL-2 and IL-15, IL-7 and its receptor, IL-7R, have been used in a variety of immunotherapy applications, often in fluid tumors and in some instances of solid tumor models (16). Sometimes use of recombinant IL-7 is preferential as current studies and early clinical trials of cancer have found less severe toxicity or side effects upon treatment with IL-7 in comparison to IL-15 or IL-2 (16).

In CAR-T cell therapies, enhanced expression and secretion of human IL-7 and CCL19 have enhanced the ability of T cells to expand and migrate in vitro (17). Engineered CAR T cells expressing IL-7 or a constitutively active IL-7R results in increased efficacy of CAR T anti-tumor effects (16, 18). IL-7 is also frequently used in combination with IL-15 as a supplement in cell culture of CAR T cells to support their expansion (19). Additionally, IL-7/IL-15 in the presence of cord blood-derived T cells helps to maintain their early differentiation state (20).

Monoclonal antibodies against IL-7R or small molecule inhibitors against the IL-7R signaling pathway are commonly used in circumstances of autoimmune diseases to delay disease progression (16).  Also due to its ability to stimulate both adaptive and innate immune cells, treatment with IL-7 has shown improved survival in patients with sepsis who are at risk of deadly secondary infections (21), providing evidence for IL-7 applications beyond cancer immunotherapy.

  1. Sasson, S.C. et al. (2006) Curr. Drug Targets 7:1571.
  2. Barata, J.T. et al. (2006) Exp. Hematol. 34:1133.
  3. Goodwin, R.G. et al. (1990) Proc. Natl. Acad. Sci. USA 86:302.
  4. Namen, A.E. et al. (1988) Nature 333:571.
  5. Shalapour, S. et al. (2012) PLoS ONE 7: e31939.
  6. Saini, M. et al. (2009) Blood 113:5793.
  7. Park, J.H. et al. (2004) Immunity 21:289.
  8. Vranjkovic, A. et al. (2007) Int. Immunol. 19:1329.
  9. Sudo, T. et al. (1993) Proc. Natl. Acad. Sci. 90:9125.
  10. Seddon, B. et al. (2003) Nat. Immunol. 4:680.
  11. Schluns, K.S. et al. (2000) Nat. Immunol. 5:426.
  12. Peschon, J.J. et al. (1994) J. Exp. Med. 180:1955.
  13. Pribyl, J.A. and T.W. LeBien (1996) Proc. Natl. Acad. Sci. 93:10348.
  14. Johnson, K. et al. (2012) J. Immunol. 188:6084.
  15. Nodland, S.E. et al. (2011) Blood 118:2116.
  16. Wang, C. et al. (2022) Int. J. Mol. Sci. 23(18).
  17. Pang, N. et al. (2021) J Hematol Oncol. 14(118).
  18. Li, L. et al. (2022) Scientific Reports. 12(12506).
  19. Xu, Y. et al. (2014) Blood. 123(24): 3750-3759.
  20. Marton, C. et al. (2022) Cancer Gene Ther. 29(7).
  21. Winer, H. et al. (2022) Cytokine. 160(156049).

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Publications for IL-7 (207-IL)(154)

We have publications tested in 5 confirmed species: Human, Mouse, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio cynocephalus (Yellow Baboon).

We have publications tested in 7 applications: Bioassay, CAR-T (Bioassay), CAR-T (Differentiation), Cell Culture, ELISA (Standard), In Vivo, Whole Cells.


Filter By Application
Bioassay
(125)
CAR-T (Bioassay)
(3)
CAR-T (Differentiation)
(1)
Cell Culture
(16)
ELISA (Standard)
(4)
In Vivo
(2)
Whole Cells
(1)
All Applications
Filter By Species
Human
(142)
Mouse
(6)
Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
(1)
Primate - Macaca mulatta (Rhesus Macaque)
(1)
Primate - Papio cynocephalus (Yellow Baboon)
(1)
All Species
Showing Publications 1 - 10 of 154. Show All 154 Publications.
Publications using 207-IL Applications Species
Huang, Y;Zhu, L;Cheng, S;Dai, R;Huang, C;Song, Y;Peng, B;Li, X;Wen, J;Gong, Y;Hu, Y;Qian, L;Zhu, L;Zhang, F;Yu, L;Yi, C;Gu, W;Ling, Z;Ma, L;Tang, W;Peng, L;Shi, G;Zhang, Y;Sun, B; Solar ultraviolet B radiation promotes ?-MSH secretion to attenuate the function of ILC2s via the pituitary-lung axis Nature communications 2023-09-12 [PMID: 37699899] (Bioassay, Mouse) Bioassay Mouse
MP Cabral-Pic, L Papagno, X Lahaye, F Perdomo-Ce, S Volant, E White, V Monceaux, S Llewellyn-, R Fromentin, DA Price, N Chomont, N Manel, A Saez-Cirio, V Appay Primary role of type I interferons for the induction of functionally optimal antigen-specific CD8+ T cells in HIV infection EBioMedicine, 2023-04-12;91(0):104557. 2023-04-12 [PMID: 37058769] (Bioassay, Human) Bioassay Human
S Li, CS Wang, A Montel-Hag, HC Chen, S Lopez, O Zhou, K Dai, S Tsai, W Satyadi, C Botero, C Wong, D Casero, GM Crooks, CS Seet Strength of CAR signaling determines T�cell versus ILC differentiation from pluripotent stem cells Cell Reports, 2023-03-11;42(3):112241. 2023-03-11 [PMID: 36906850] (Bioassay, Human) Bioassay Human
T Tate, S Matsumoto, K Nemoto, M Leisegang, S Nagayama, K Obama, Y Nakamura, K Kiyotani Identification of T Cell Receptors Targeting a Neoantigen Derived from Recurrently Mutated FGFR3 Cancers, 2023-02-06;15(4):. 2023-02-06 [PMID: 36831375] (Bioassay, Human) Bioassay Human
D Proietto, B Dallan, E Gallerani, V Albanese, S Llewellyn-, DA Price, V Appay, S Pacifico, A Caputo, F Nicoli, R Gavioli Ageing Curtails the Diversity and Functionality of Nascent CD8+ T Cell Responses against SARS-CoV-2 Vaccines, 2023-01-11;11(1):. 2023-01-11 [PMID: 36679999] (Cell Culture, Human) Cell Culture Human
Y Kitamura, YI Koma, K Tanigawa, S Tsukamoto, Y Azumi, S Miyako, S Urakami, T Kodama, M Nishio, M Shigeoka, Y Kakeji, H Yokozaki Roles of IL-7R Induced by Interactions between Cancer Cells and Macrophages in the Progression of Esophageal Squamous Cell Carcinoma Cancers, 2023-01-06;15(2):. 2023-01-06 [PMID: 36672342] (Bioassay, Human) Bioassay Human
YS Michaels, JM Edgar, MC Major, EL Castle, C Zimmerman, T Yin, A Hagner, C Lau, HH Hsu, MI Ibañez-Rio, LJ Durland, DJHF Knapp, PW Zandstra DLL4 and VCAM1 enhance the emergence of T cell-competent hematopoietic progenitors from human pluripotent stem cells Science Advances, 2022-08-24;8(34):eabn5522. 2022-08-24 [PMID: 36001668] (Bioassay, Human) Bioassay Human
R Jing, I Scarfo, MA Najia, E Lummertz d, A Han, M Sanborn, T Bingham, C Kubaczka, DK Jha, M Falchetti, TM Schlaeger, TE North, MV Maus, GQ Daley EZH1 repression generates mature iPSC-derived CAR T�cells with enhanced antitumor activity Cell Stem Cell, 2022-08-04;29(8):1181-1196.e6. 2022-08-04 [PMID: 35931029] (Bioassay, Human) Bioassay Human
P Sekhri, DK Ledezma, A Shukla, EE Sweeney, R Fernandes The Thermal Dose of Photothermal Therapy Generates Differential Immunogenicity in Human Neuroblastoma Cells Cancers, 2022-03-11;14(6):. 2022-03-11 [PMID: 35326601] (Whole Cells, Human) Whole Cells Human
GR Campbell, J Zhuang, G Zhang, I Landa, LJ Kubiatowic, D Dehaini, RH Fang, L Zhang, SA Spector CD4+ T cell-mimicking nanoparticles encapsulating DIABLO/SMAC mimetics broadly neutralize HIV-1 and selectively kill HIV-1-infected cells Theranostics, 2021-08-25;11(18):9009-9021. 2021-08-25 [PMID: 34522224] (Bioassay, Human) Bioassay Human
Show All 154 Publications.

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Bioinformatics

Gene Symbol IL7
Uniprot