IL-17/IL-17A Antibody [Biotin]

• Reactivity: Mu
• Applications: WB, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

IL-17/IL-17A Antibody [Biotin] Summary

• Immunogen: E. coli-derived recombinant mouse IL‑17 (R&D Systems, Catalog # 421-ML)
  Thr22-Ala158
  Accession # Q62386
• Specificity: Detects mouse IL-17 in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant human IL‑17 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: IL17A
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse IL-17 Antibody (Catalog # MAB721)
  ELISA Detection: Mouse IL-17 Biotinylated Antibody (Catalog # BAF421)
  Standard: Recombinant Mouse IL-17 (Catalog # 421-ML)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IL-17/IL-17A Antibody [Biotin]

• CTLA8
• CTLA-8
• CTLA8cytotoxic T-lymphocyte-associated serine esterase 8
• Cytotoxic T-lymphocyte-associated antigen 8
• IL17
• IL-17
• IL17A
• IL-17A
• IL-17Acytotoxic T-lymphocyte-associated protein 8
• IL-17CTLA-8
• IL17interleukin-17A
• interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)
• interleukin 17A

Background

Interleukin 17 (also known as CTLA-8) is a T cell-expressed pleiotropic cytokine that exhibits a high degree of homology to a protein encoded by the ORF13 gene of herpesvirus Saimiri. cDNA clones encoding IL-17 have been isolated from activated rat, mouse and human T cells. Mouse IL-17 cDNA encodes a 158 amino acid (aa) residue precursor protein with a 21 amino acid residue signal peptide that is cleaved to yield the 137 aa residue mature  IL-17. Both recombinant and natural IL-17 have been shown to exist as disulfide linked homodimers. At the amino acid level, mouse IL-17 shows 57% and 87% sequence identity with herpes virus and rat IL-17, respectively. An IL-17 specific mouse cell surface receptor (IL-17 R) has been cloned. While the expression of IL-17 mRNA is restricted to activated alpha beta TCR⁺CD4^-CD8^- T cells, the expression of mouse IL-17 R mRNA has been detected in virtually all cells and tissues tested. IL-17 exhibits multiple biological activities on a variety of cells including: the induction of IL-6 and IL-8 production in fibroblasts; the enhancement of surface expression of ICAM-1 in fibroblasts; activation of NF-kappa B and costimulation of T cell proliferation.

1. Kennedy, J. et al. (1996) J. Interferon Cytokine Res. 16:611.
2. Yao, Z. et al. (1995) J. Immunol. 155:5483.
3. Yao, Z. et al. (1995) Immunity 3:811.
4. Rouvier, E. et al. (1993) J. Immunol. 150:5445.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IL-17/IL-17A Antibody (BAF421)(23)

Publications using BAF421

• K Kasahara, N Sasaki, HZ Amin, T Tanaka, S Horibe, T Yamashita, KI Hirata, Y Rikitake Depletion of Foxp3+ regulatory T cells augments CD4+ T cell immune responses in atherosclerosis-prone hypercholesterolemic mice Heliyon, 2022-07-19;8(7):e09981. 2022-07-19 [PMID: 35898604] (ELISA Detection, Mouse)
• SH Lee, JY Kwon, SY Kim, K Jung, ML Cho Interferon-gamma regulates inflammatory cell death by targeting necroptosis in experimental autoimmune arthritis Sci Rep, 2017-08-31;7(1):10133. 2017-08-31 [PMID: 28860618] (ELISA Development (Detection), Mouse)
• R Lv, J Zhao, M Lei, D Xiao, Y Yu, J Xie IL-33 Attenuates Sepsis by Inhibiting IL-17 Receptor Signaling through Upregulation of SOCS3 Cell. Physiol. Biochem., 2017-08-09;42(5):1961-1972. 2017-08-09 [PMID: 28793286] (ELISA Development (Detection), Mouse)
• AC Yau, J Tuncel, R Holmdahl The Major Histocompatibility Complex Class III Haplotype Ltab-Ncr3 Regulates Adjuvant-Induced but Not Antigen-Induced Autoimmunity Am. J. Pathol, 2017-03-18;0(0):. 2017-03-18 [PMID: 28315676] (ELISA Development (Detection), Rat)
• MHC class II alleles associated with Th1 rather than Th17 type immunity drive the onset of early arthritis in a rat model of rheumatoid arthritis Eur. J. Immunol, 2017-02-14;0(0):. 2017-02-14 [PMID: 28012172] (ELISA Development (Detection), Rat)
• Jung-Yeon Lim Enhanced immunoregulation of mesenchymal stem cells by IL-10-producing type 1 regulatory T cells in collagen-induced arthritis Sci Rep, 2016-06-01;6(0):26851. 2016-06-01 [PMID: 27246365] (ELISA Development (Detection), Mouse)
• Haag S, Tuncel J, Thordardottir S, Mason D, Yau A, Dobritzsch D, Backlund J, Peters E, Holmdahl R Positional identification of RT1-B (HLA-DQ) as susceptibility locus for autoimmune arthritis. J Immunol, 2015-02-11;194(6):2539-50. 2015-02-11 [PMID: 25672758] (ELISA Development (Detection), Rat)
• Long Hang, Arthur M. Blum, Tommy Setiawan, Joseph P. Urban, Korynn M. Stoyanoff, Joel V. Weinstock Heligmosomoides polygyrus bakeri Infection Activates Colonic Foxp3+ T Cells Enhancing Their Capacity To Prevent Colitis The Journal of Immunology 2013-08-15 [PMID: 23851695] (ELISA Capture, Mouse)
• Lee J, Lee J, Park M, Lim M, Park E, Kim E, Yang E, Lee S, Jhun J, Park S, Kim H, Cho M Interferon gamma suppresses collagen-induced arthritis by regulation of Th17 through the induction of indoleamine-2,3-deoxygenase. PLoS ONE, 2013-04-16;8(4):e60900. 2013-04-16 [PMID: 23613752] (ELISA Development (Detection), Mouse)
• Costa MF, Bornstein VU, Candea AL, Henriques-Pons A, Henriques MG, Penido C CCL25 induces alpha 4 beta 7 integrin-dependent migration of IL-17+ gamma/delta T lymphocytes during an allergic reaction. Eur. J. Immunol., 2012-05-01;42(5):1250-60. 2012-05-01 [PMID: 22539297] (ELISA Development, Mouse)
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)
• John Leung, Long Hang, Arthur Blum, Tommy Setiawan, Korynn Stoyanoff, Joel Weinstock Heligmosomoides Polygyrus Abrogates Antigen-Specific Gut Injury in a Murine Model of Inflammatory Bowel Disease Inflammatory Bowel Diseases 2012-08-01 [PMID: 22223533]
• Martin Beinborn, Arthur Blum, Long Hang, Tommy Setiawan, Jonathan C. Schroeder, Korynn Stoyanoff et al. TGF-beta regulates T-cell neurokinin-1 receptor internalization and function Proceedings of the National Academy of Sciences 2010-03-02 [PMID: 20160079]
• Lemos HP, Grespan R, Vieira SM, Cunha TM, Verri WA, Fernandes KS, Souto FO, McInnes IB, Ferreira SH, Liew FY, Cunha FQ Prostaglandin mediates IL-23/IL-17-induced neutrophil migration in inflammation by inhibiting IL-12 and IFNgamma production. Proc. Natl. Acad. Sci. U.S.A., 2009-03-16;106(14):5954-9. 2009-03-16 [PMID: 19289819] (ELISA Development, Mouse)
• Sawa Y, Arima Y, Ogura H, Kitabayashi C, Jiang JJ, Fukushima T, Kamimura D, Hirano T, Murakami M Hepatic interleukin-7 expression regulates T cell responses. Immunity, 2009-03-12;30(3):447-57. 2009-03-12 [PMID: 19285437] (Western Blot, Mouse)
• Yang H, Gumucio DL, Teitelbaum DH Intestinal specific overexpression of interleukin-7 attenuates the alternation of intestinal intraepithelial lymphocytes after total parenteral nutrition administration. Ann. Surg., 2008-11-01;248(5):849-56. 2008-11-01 [PMID: 18948814] (Western Blot, Mouse)
• Muranski P, Boni A, Antony PA, Cassard L, Irvine KR, Kaiser A, Paulos CM, Palmer DC, Touloukian CE, Ptak K, Gattinoni L, Wrzesinski C, Hinrichs CS, Kerstann KW, Feigenbaum L, Chan CC, Restifo NP Tumor-specific Th17-polarized cells eradicate large established melanoma. Blood, 2008-03-19;112(2):362-73. 2008-03-19 [PMID: 18354038] (ELISA Development, Mouse)
• O'Toole M, Legault H, Ramsey R, Wynn TA, Kasaian MT A novel and sensitive ELISA reveals that the soluble form of IL-13R-alpha2 is not expressed in plasma of healthy or asthmatic subjects. Clin. Exp. Allergy, 2008-02-25;38(4):594-601. 2008-02-25 [PMID: 18307523] (ELISA Development, Human)
• Sheibanie AF, Yen JH, Khayrullina T, Emig F, Zhang M, Tuma R, Ganea D The proinflammatory effect of prostaglandin E2 in experimental inflammatory bowel disease is mediated through the IL-23-->IL-17 axis. J. Immunol., 2007-06-15;178(12):8138-47. 2007-06-15 [PMID: 17548652] (ELISA Development, Mouse)
• Lockhart E, Green AM, Flynn JL IL-17 production is dominated by gammadelta T cells rather than CD4 T cells during Mycobacterium tuberculosis infection. J. Immunol., 2006-10-01;177(7):4662-9. 2006-10-01 [PMID: 16982905] (ELISpot Development, Mouse)
• Spencer C. Liang, Xiang-Yang Tan, Deborah P. Luxenberg, Riyez Karim, Kyriaki Dunussi-Joannopoulos, Mary Collins et al. Interleukin (IL)-22 and IL-17 are coexpressed by Th17 cells and cooperatively enhance expression of antimicrobial peptides The Journal of Experimental Medicine 2006-10-02 [PMID: 16982811]
• Miyamoto M, Prause O, Sjostrand M, Laan M, Lotvall J, Linden A Endogenous IL-17 as a mediator of neutrophil recruitment caused by endotoxin exposure in mouse airways. J. Immunol., 2003-05-01;170(9):4665-72. 2003-05-01 [PMID: 12707345] (ELISpot Development, Mouse)
• Lubberts E, Joosten LA, Oppers B, van den Bersselaar L, Coenen-de Roo CJ, Kolls JK, Schwarzenberger P, van de Loo FA, van den Berg WB IL-1-independent role of IL-17 in synovial inflammation and joint destruction during collagen-induced arthritis. J. Immunol., 2001-07-15;167(2):1004-13. 2001-07-15 [PMID: 11441109] (ELISA Development, Mouse)

Reviews for IL-17/IL-17A Antibody (BAF421) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer ELISA Mouse 01/14/2015

Summary

• Application: ELISA
• Sample Tested: See PMID: 22491249
• Species: Mouse

reviewed by: Verified Customer ELISA Mouse 01/14/2015

Summary

• Application: ELISA
• Sample Tested: See PMID: 21469121
• Species: Mouse

reviewed by: Verified Customer ELISA Mouse 01/14/2015

Summary

• Application: ELISA
• Sample Tested: See PMID: 21398615
• Species: Mouse

FAQs for IL-17/IL-17A Antibody (BAF421). (Showing 1 - 2 of 2 FAQs).

1. I want to stain some T cell subsets (Th17 and Th1), do you have antibodies for that? I need for flow cytometer in sheep or goat.
   • Unfortunately, we have neither a CD4 antibody nor an IL-17 antibody that has been validated in sheep or goat, although if you would like to try one you would again be eligible for our Innovators Reward Program. Please contact us at innovators@novusbio.com with any questions regarding this program.
2. I'm looking to establish an ELISA for IL-17 and want your recommendation on which antibody pairs to buy. Least expensive ones, please. Also, I'd like the detection antibody to be fluorescently labeled.
   • Thank you for your patience while I reviewed our ELISA validated products for IL17. I was not sure what species you were going to be investigating, so I looked up our data for the human protein. If this is incorrect, just let me know and I can look up products for the species you will be working with. As for antibody pairs, we only had one set that have been tested together: Capture: NBP2-22531 Detection: NBP2-22532. The detection product is only available currently with a biotin conjugate. We can however offer custom conjugation for this product to any of our available conjugates. Please see the this link to see the available fluors. The typical fee for custom conjugation is usually about $80 additional. Once you know which fluor you would like, I can provide you with a quote. We do also offer several ELISA kits for this target (they typically employ HRP or Biotin detection systems, so I am not sure if they will work for your needs).

Bioinformatics

• Gene Symbol: IL17A
• Uniprot:
  • Q62386()