IL-1 beta/IL-1F2 Antibody

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Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Detects 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1Beta containing samples but will primarily detect all of the 17,000 MW mature molecule. ...read more
Immunocytochemistry/ Immunofluorescence: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Staining of mouse carotid artery. A) No Ab staining of WT uninjured Ms. B) IL-1 beta staining of cells after surgical injury of tissue. ...read more
Immunohistochemistry: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis of Mouse Embryonic Kidney Tissue: Mouse Embryonic Kidney Fixation: FFPE buffered formalin 10% conc Ag Retrieval: Heat, Citrate pH 6.2. Pressure ...read more
Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the Biotin conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1beta Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 ...read more
Western Blot: IL-1 beta/IL-1F2 Antibody [NBP1-42767] - Analysis using the HRP conjugate of NBP1-42767. Detection of Lane 1: Mouse IL-1 Beta Load: 50 ug per Detection of Lane Secondary antibody: IL1 beta Antibody ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP
Clonality
Polyclonal
Host
Rabbit
Conjugate
Unconjugated
Concentration
LYOPH

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IL-1 beta/IL-1F2 Antibody Summary

Description
This is an IgG preparation of whole rabbit serum purified by DEAE fractionation.
Immunogen
This antibody was prepared by repeated immunizations with recombinant mouse IL-1 beta produced in E.coli. The MW of recombinant mouse IL-1 beta was 17 kDa.
Specificity
This antibody is primarily directed against mature, 17,000 MW mouse IL-1 beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1 beta or mouse IL-1a. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1 beta. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL1B
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:500-1:2000
  • ELISA 1:1000-1:5000
  • Flow Cytometry 1:10-1:1000
  • Immunocytochemistry/Immunofluorescence 1:50-1:250
  • Immunohistochemistry 1:50-1:250
  • Immunohistochemistry-Paraffin 1:10-1:500
  • Immunoprecipitation 1:200-1:800
Application Notes
This antibody recognizes the 17,000 MW mature IL-1Beta. For immunoblots, typically, IL-1Beta is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells. PBMC are stimulated for 24 hours with 1% serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG - reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation - stain intracellular IL-1Beta. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of mouse and rat IL-1Beta activity in bioassays. It does not neutralize the biological activity IL-1alpha. It does not neutralize the biological activity of human or primate IL-1Beta. For neutralization, it is recommended - incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used f. Caution should be exhibited as the F domain of the rabbit IgG molecule may interact with cells non-specifically.
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Reviewed Applications
Read 1 Review rated 5
using
NBP1-42767 in the following applications:

Publications
Read Publications using
NBP1-42767 in the following applications:

  • 1 publication
  • 1 publication
  • 1 publication
  • WB
    1 publication

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
Preservative
No Preservative
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Alternate Names for IL-1 beta/IL-1F2 Antibody

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 beta (also known as Interleukin-1 beta, IL-1 beta and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1 beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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NBP1-42767
Species: Mu, Rt
Applications: WB, ELISA, Flow, ICC/IF, IHC, IHC-P, IP

Publications for IL-1 beta/IL-1F2 Antibody (NBP1-42767)(6)

We have publications tested in 2 confirmed species: Mouse, Rat.

We have publications tested in 4 applications: FLOW, Flow, IHC-P, WB.


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Showing Publications 1 - 6 of 6.
Publications using NBP1-42767 Applications Species
Hardas SS, Sultana R, Warrier G et al. Rat hippocampal responses up to 90 days after a single nanoceria dose extends a hierarchical oxidative stress model for nanoparticle toxicity. Nanotoxicology 2013 Dec 18 [PMID: 24350865] (WB, Rat) WB Rat
Triantafilou K, Kar S, van Kuppeveld FJ, Triantafilou M. Rhinovirus-Induced Calcium Flux Triggers NLRP3 and NLRC5 Activation in Bronchial Cells. Am J Respir Cell Mol Biol 2013 Jul 1 [PMID: 23815151]
Fujisaka S, Usui I, Ikutani M et al. Adipose tissue hypoxia induces inflammatory M1 polarity of macrophages in an HIF-1alpha-dependent and HIF-1alpha-independent manner in obese mice Diabetologia 2013 Mar 15 [PMID: 23494472] (Flow, Mouse)

Details:
This citation used the Biotin version of this antibody.
Flow Mouse
Triantafilou K, Kar S, Vakakis E et al. Human respiratory syncytial virus viroporin SH: a viral recognition pathway used by the host to signal inflammasome activation Thorax 2013 Jan [PMID: 23229815]
Reed M. Requirement of autophagy proteins Beclin-1 and Map1-LC3b in the induction of innate and adaptive immune responses to respiratory syncytial virus infection Thesis. 2014 (IHC-P, Mouse) IHC-P Mouse
Fujisaka S, Usui I, Ikutani M et al. Adipose tissue hypoxia induces inflammatory M1 polarity of macrophages in an HIF-1alpha-dependent and HIF-1alpha-independent manner in obese mice Diabetologia (FLOW, Mouse)

Details:
This publication used the Biotin conjugated form of this antibody (Cat# NBP1-42768).
FLOW Mouse

Review for IL-1 beta/IL-1F2 Antibody (NBP1-42767) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow.

Reviews using NBP1-42767:
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Images Ratings Applications Species Date Details
Flow Cytometry IL-1 beta/IL-1F2 NBP1-42767
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reviewed by:
Jim Hsiao
Flow Mouse 01/23/2017
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Summary

ApplicationFlow Cytometry
Sample TestedLung tissue
SpeciesMouse
Lot34448

Comments

CommentsSingle cells were isolated from a mouse lung graft and stained with IL-1b ab (1:00 in FACS buffer) for 30 minutes. Cells were then washed and stained with Goat anti-rabbit FITC-conjugated secondary antibody for additional 30 minutes. Cells were then washed and analyzed by flow cytometry.

Product General Protocols

Video Protocols

WB Video Protocol
ICC/IF Video Protocol

FAQs for IL-1 beta/IL-1F2 Antibody (NBP1-42767). (Showing 1 - 2 of 2 FAQs).

  1. Do you have a protocol for guiding me how to prepare the solution of this antibody?
    • Our reconstitution instructions for the lyophilized antibody NBP1-42767 (IL1 beta Antibody) are 'restore with 100 ul deionized water (or equivalent)'.
  2. Can I distribute the solution as 10 ul per tube, 10 tubes in total, and store them in -80 degree Celsius refrigerator?
    • Yes, that should be okay, although we tend to recommend storing this antibody at -20 degrees Celsius. Aliquoting is a good idea because freeze-thaw cycles should be avoided.

Secondary Antibodies

 

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Bioinformatics Tool for IL-1 beta/IL-1F2 Antibody (NBP1-42767)

Discover related pathways, diseases and genes to IL-1 beta/IL-1F2 Antibody (NBP1-42767). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
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Diseases for IL-1 beta/IL-1F2 Antibody (NBP1-42767)

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Pathways for IL-1 beta/IL-1F2 Antibody (NBP1-42767)

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PTMs for IL-1 beta/IL-1F2 Antibody (NBP1-42767)

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Research Areas for IL-1 beta/IL-1F2 Antibody (NBP1-42767)

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Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Recent Reviews

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Jim Hsiao
01/23/2017
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol IL1B
Entrez
Uniprot