Human IL-1ra/IL-1F3 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Catalog# & Conjugate Size Price

Human IL-1ra/IL-1F3 Quantikine ELISA Kit Summary

Background
The Quantikine Human IL-1ra Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-1ra in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human IL-1ra as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-1ra. Results obtained using natural human IL-1r...a showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1ra.
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Specificity
Natural and recombinant human IL-1ra
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL1RN

Applications/Dilutions

Dilutions
  • ELISA
Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using DRA00B.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-1ra/IL-1F3 Quantikine ELISA Kit

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4

Background

Interleukin-1 receptor antagonist (IL-1ra; also known as IL-1F3) is a 22-25 kDa member of the IL-1 family of cytokines. Currently, there are 11 family members (IL-1F1-F11), nine of which form an IL-1 gene cluster on human Ch2 (1-3). Each IL-1 family member contains an IL-1 fold. This fold is generated by 12 packed beta -sheets that interact to form a beta -trefoil structure. Little amino acid (aa) homology is required to achieve this structure, and this explains the low aa identity among IL-1 family members. IL-1ra is a pure cytokine receptor antagonist that has no signal transduction-initiating activity (4). It is an acute phase protein that exists to dampen inflammation. IL-1( beta ) is initially produced by monocytes in response to a variety of stimuli. Circulating IL-1 then binds to widely expressed IL-1 type I receptors (IL-1 RI) and initiates a number of pro-inflammatory events. On endothelial cells (EC), IL-1 induces PGE2 and IL-6 release, generating fever, thrombocytosis, and hepatic acute phase protein production. In synovial joints, IL-1 induces chondrocyte NO production, an event that leads to reduced collagen synthesis and chondrocyte apoptosis. Finally, IL-1 increases neutrophil counts, both in blood and tissue, and thus is able to promote a pro-inflammatory environment in multiple locations (5-8). IL-1ra blocks IL-1 action through competitive inhibition. More correctly, although IL-1ra fills the IL-1 binding site in IL-1 RI, it is also unable to orchestrate the creation of a signal-transducing IL-1 RI:IL-1 R Accessory protein (IL-1 R AcP) heterodimer complex. Effective IL-1ra concentrations are generally 100-fold greater than local IL-1 concentrations. This is because the IL-1ra half-life is but 6 minutes, and very few IL-1 type I receptors need to be engaged by IL-1 to elicit a cellular response (5, 7, 9). 
Human IL-1ra is synthesized as a 177 aa precursor that contains a 25 aa signal sequence and a 152 aa mature region (10, 11). Although it contains an IL-1 cytokine fold, it apparently lacks two structural motifs that allow for activation of the IL-1 receptor heterodimer. First, and following binding to IL-1 RI, the presence of Ile 51-His 54 and Lys 145 of the mature molecule preclude recruitment of IL-1 R AcP. Second, there is no identifiable C-terminal lectin segment that is hypothesized to help recruit an accessory signaling component (1, 12, 13). Mature human IL-1ra is 77% and 82% aa identical to mouse and canine IL-1ra, respectively, and human IL-1ra inhibits IL-1 activity on mouse cells (10). A number of cell types express IL-1ra, including monocytes (11), Sertoli cells (14), hepatocytes (15), adipocytes (16), synovial fibroblasts (17), mast cells (18), pancreatic beta -cells (19), and intestinal epithelial cells (20). There are at least three intracellular IL-1ra isoforms (icIL-1ra1, 2, and 3). All show N-terminal variation, and all contain amino acids 35-177 of the secreted precursor (21-23). Intracellular IL-1ra1 is of particular interest, because it is reported to be "secreted" by endothelial cells and binds to the IL-1 RI in an antagonist fashion (23-25). Intracellular IL-1ra1 is 159 aa in length and shows a 3 aa substitution for the first 21 aa's of the signal sequence of IL-1ra (21).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-1ra/IL-1F3/IL1RN (DRA00B)(87)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Ovine, Primate, Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio hamadryas (Hamadyras Baboon).


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Human
(79)
Mouse
(1)
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(1)
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(1)
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(1)
Primate - Macaca mulatta (Rhesus Macaque)
(2)
Primate - Papio hamadryas (Hamadyras Baboon)
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Showing Publications 1 - 10 of 87. Show All 87 Publications.
Publications using DRA00B Applications Species
DB Rosen, EA Murphy, RS Gejman, A Capili, RL Friedlande, S Rand, KA Cagino, SM Glynn, KC Matthews, JM Kubiak, J Yee, M Prabhu, LE Riley, YJ Yang Cytokine response over the course of COVID-19 infection in pregnant women Cytokine, 2022;154(0):155894. 2022 [PMID: 35490452] (Human) Human
D Martins, O Dipasquale, K Davies, E Cooper, J Tibble, M Veronese, M Frigo, SCR Williams, F Turkheimer, M Cercignani, NA Harrison Transcriptomic and cellular decoding of functional brain connectivity changes reveal regional brain vulnerability to pro- and anti-inflammatory therapies Brain, Behavior, and Immunity, 2022;102(0):312-323. 2022 [PMID: 35259429] (Human) Human
M Archer, O Niemelä, M Hämäläinen, E Moilanen, E Leinonen, O Kampman The role of alcohol use and adiposity in serum levels of IL-1RA in depressed patients BMC Psychiatry, 2022;22(1):158. 2022 [PMID: 35232419] (Human) Human
C Gar, B Thorand, C Herder, C Sujana, M Heier, C Meisinger, A Peters, W Koenig, W Rathmann, M Roden, M Stumvoll, H Maalmi, T Meitinger, H Then, J Seissler, C Then Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study PLoS ONE, 2022;17(1):e0262330. 2022 [PMID: 34990470] (Human) Human
K Burrows, JL Stewart, R Kuplicki, L Figueroa-H, PA Spechler, H Zheng, SM Guinjoan, Tulsa 1000, JB Savitz, T Kent Teagu, MP Paulus Elevated peripheral inflammation is associated with attenuated striatal reward anticipation in major depressive disorder Brain, Behavior, and Immunity, 2021;0(0):. 2021 [PMID: 33508469] (Human) Human
S Frankova, M Lunova, H Gottfriedo, R Senkerikov, M Neroldova, J Kovac, E Kieslichov, V Lanska, P Urbanek, J Spicak, M Jirsa, J Sperl Liver stiffness measured by two-dimensional shear-wave elastography predicts hepatic vein pressure gradient at high values in liver transplant candidates with advanced liver cirrhosis PLoS ONE, 2021;16(1):e0244934. 2021 [PMID: 33411729] (Human) Human
N Kashiwagi, F Saito, H Maegawa, K Kaneda Granulocyte and monocyte adsorptive apheresis induces apoptosis of neutrophils and release of a novel chemoattractant for desensitization of interleukin-8 response - In vitro and in vivo results Cytokine, 2021;139(0):155410. 2021 [PMID: 33395614] (Human) Human
S Ballikaya, S Sadeghi, E Niebergall, L Nimtz, J Frindert, A Norrick, N Stemler, N Bauer, Y Rosche, V Kratzenber, J Pieper, T Ficek, MH Frank, C Ganss, J Esterlechn, MA Kluth Process data of allogeneic ex vivo-expanded ABCB5+ mesenchymal stromal cells for human use: off-the-shelf GMP-manufactured donor-independent ATMP Stem Cell Res Ther, 2020;11(1):482. 2020 [PMID: 33198791] (Human) Human
DM de Graaf, M Jaeger, ICL van den Mu, RT Horst, K Schraa, J Zwaag, M Kox, M Fujita, T Yamauchi, L Mercurio, S Madonna, JHW Rutten, J de Graaf, NP Riksen, FL van de Vee, MG Netea, LAB Joosten, CA Dinarello Reduced concentrations of the B cell cytokine Interleukin 38 are associated with cardiovascular disease risk in overweight subjects Eur J Immunol, 2020;0(0):. 2020 [PMID: 33125159]
T Peeters, S Martens, V D'Onofrio, MHT Stappers, JCH van der Hi, B Houben, R Achten, LAB Joosten, IC Gyssens An observational study of innate immune responses in patients with acute appendicitis Sci Rep, 2020;10(1):17352. 2020 [PMID: 33060696] (Human) Human
Show All 87 Publications.

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Bioinformatics

Gene Symbol IL1RN