Human IL-1ra/IL-1F3 Quantikine ELISA Kit

Product Details

Summary
Reactivity HuSpecies Glossary
Applications ELISA
Conjugate
HRP

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Catalog# & Conjugate Size Price

Human IL-1ra/IL-1F3 Quantikine ELISA Kit Summary

Background
The Quantikine Human IL-1ra Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-1ra in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human IL-1ra as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-1ra. Results obtained using natural human IL-1r...a showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1ra.
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Specificity
Natural and recombinant human IL-1ra
Source
N/A
Inter-Assay
See PDF Datasheet for details
Intra-Assay
See PDF Datasheet for details
Spike Recovery
See PDF Datasheet for details
Sample Volume
See PDF Datasheet for details
Gene
IL1RN

Applications/Dilutions

Application Notes
Interference observed with 1 or more available related molecules.
Publications
Read Publications using DRA00B.

Packaging, Storage & Formulations

Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-1ra/IL-1F3 Quantikine ELISA Kit

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4

Background

Interleukin-1 receptor antagonist (IL-1ra; also known as IL-1F3) is a 22-25 kDa member of the IL-1 family of cytokines. Currently, there are 11 family members (IL-1F1-F11), nine of which form an IL-1 gene cluster on human Ch2 (1-3). Each IL-1 family member contains an IL-1 fold. This fold is generated by 12 packed beta -sheets that interact to form a beta -trefoil structure. Little amino acid (aa) homology is required to achieve this structure, and this explains the low aa identity among IL-1 family members. IL-1ra is a pure cytokine receptor antagonist that has no signal transduction-initiating activity (4). It is an acute phase protein that exists to dampen inflammation. IL-1( beta ) is initially produced by monocytes in response to a variety of stimuli. Circulating IL-1 then binds to widely expressed IL-1 type I receptors (IL-1 RI) and initiates a number of pro-inflammatory events. On endothelial cells (EC), IL-1 induces PGE2 and IL-6 release, generating fever, thrombocytosis, and hepatic acute phase protein production. In synovial joints, IL-1 induces chondrocyte NO production, an event that leads to reduced collagen synthesis and chondrocyte apoptosis. Finally, IL-1 increases neutrophil counts, both in blood and tissue, and thus is able to promote a pro-inflammatory environment in multiple locations (5-8). IL-1ra blocks IL-1 action through competitive inhibition. More correctly, although IL-1ra fills the IL-1 binding site in IL-1 RI, it is also unable to orchestrate the creation of a signal-transducing IL-1 RI:IL-1 R Accessory protein (IL-1 R AcP) heterodimer complex. Effective IL-1ra concentrations are generally 100-fold greater than local IL-1 concentrations. This is because the IL-1ra half-life is but 6 minutes, and very few IL-1 type I receptors need to be engaged by IL-1 to elicit a cellular response (5, 7, 9). 
Human IL-1ra is synthesized as a 177 aa precursor that contains a 25 aa signal sequence and a 152 aa mature region (10, 11). Although it contains an IL-1 cytokine fold, it apparently lacks two structural motifs that allow for activation of the IL-1 receptor heterodimer. First, and following binding to IL-1 RI, the presence of Ile 51-His 54 and Lys 145 of the mature molecule preclude recruitment of IL-1 R AcP. Second, there is no identifiable C-terminal lectin segment that is hypothesized to help recruit an accessory signaling component (1, 12, 13). Mature human IL-1ra is 77% and 82% aa identical to mouse and canine IL-1ra, respectively, and human IL-1ra inhibits IL-1 activity on mouse cells (10). A number of cell types express IL-1ra, including monocytes (11), Sertoli cells (14), hepatocytes (15), adipocytes (16), synovial fibroblasts (17), mast cells (18), pancreatic beta -cells (19), and intestinal epithelial cells (20). There are at least three intracellular IL-1ra isoforms (icIL-1ra1, 2, and 3). All show N-terminal variation, and all contain amino acids 35-177 of the secreted precursor (21-23). Intracellular IL-1ra1 is of particular interest, because it is reported to be "secreted" by endothelial cells and binds to the IL-1 RI in an antagonist fashion (23-25). Intracellular IL-1ra1 is 159 aa in length and shows a 3 aa substitution for the first 21 aa's of the signal sequence of IL-1ra (21).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Publications for IL-1ra/IL-1F3/IL1RN (DRA00B)(67)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Ovine, Primate, Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio hamadryas (Hamadyras Baboon).


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(1)
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(2)
Primate - Papio hamadryas (Hamadyras Baboon)
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Showing Publications 1 - 10 of 67. Show All 67 Publications.
Publications using DRA00B Applications Species
S Lucht, F Hennig, S Moebus, S Ohlwein, C Herder, B Kowall, KH Jöckel, B Hoffmann All-source and source-specific air pollution and 10-year diabetes Incidence: Total effect and mediation analyses in the Heinz Nixdorf recall study Environ Int, 2020;136(0):105493. 2020 [PMID: 31991234] (Human) Human
S Lucht, F Hennig, S Moebus, D Führer-Sak, C Herder, KH Jöckel, B Hoffmann, Air pollution and diabetes-related biomarkers in non-diabetic adults: A pathway to impaired glucose metabolism? Environ Int, 2019;124(0):370-392. 2019 [PMID: 30660850] (Human) Human
W Coryell, H Wilcox, SJ Evans, GN Pandey, L Jones-Bran, F Dickerson, R Yolken Aggression, impulsivity and inflammatory markers as risk factors for suicidal behavior J Psychiatr Res, 2018;106(0):38-42. 2018 [PMID: 30261413] (Human) Human
DT Skelly, ÉW Griffin, CL Murray, S Harney, C O'Boyle, E Hennessy, MA Dansereau, A Nazmi, L Tortorelli, JN Rawlins, DM Bannerman, C Cunningham Acute transient cognitive dysfunction and acute brain injury induced by systemic inflammation occur by dissociable IL-1-dependent mechanisms Mol. Psychiatry, 2019;24(10):1533-1548. 2019 [PMID: 29875474] (Mouse) Mouse
KC El Kasmi, PM Vue, AL Anderson, MW Devereaux, S Ghosh, N Balasubram, SA Fillon, C Dahrenmoel, A Allawzi, C Woods, S McKenna, CJ Wright, L Johnson, A D'Alessand, JA Reisz, E Nozik-Gray, FJ Suchy, RJ Sokol Macrophage-derived IL-1?/NF-?B signaling mediates parenteral nutrition-associated cholestasis Nat Commun, 2018;9(1):1393. 2018 [PMID: 29643332] (Human) Human
P Presicce, CW Park, P Senthamara, S Bhattachar, C Jackson, F Kong, CM Rueda, E DeFranco, LA Miller, DA Hildeman, N Salomonis, CA Chougnet, AH Jobe, SG Kallapur IL-1 signaling mediates intrauterine inflammation and chorio-decidua neutrophil recruitment and activation JCI Insight, 2018;3(6):. 2018 [PMID: 29563340] (Primate - Macaca mulatta (Rhesus Macaque)) Primate - Macaca mulatta (Rhesus Macaque)
C Herder, A Schmitt, F Budden, A Reimer, B Kulzer, M Roden, T Haak, N Hermanns Association between pro- and anti-inflammatory cytokines and depressive symptoms in patients with diabetes-potential differences by diabetes type and depression scores Transl Psychiatry, 2018;7(11):1. 2018 [PMID: 29520075] (Human) Human
PM Macedo, R Almeida-Pa, MA Almeida, RA Coelho, MA Oliveira F, DM Medeiros, A Gomes-Silv, JR Lima, AM Da-Cruz, RM Zancopé-Ol, ACFD Valle Fatal septic shock caused by Paracoccidioides brasiliensis phylogenetic species S1 in a young immunocompetent patient: a case report Rev. Soc. Bras. Med. Trop., 2018;51(1):111-114. 2018 [PMID: 29513831] (Human) Human
N Schofer, S Ludwig, N Rübsamen, R Schnabel, KJ Lackner, HJ Ruprecht, C Bickel, U Landmesser, S Blankenber, T Zeller Prognostic impact of Interleukin-1 receptor antagonist in patients with documented coronary artery disease Int. J. Cardiol., 2018;0(0):. 2018 [PMID: 29395365] (Human) Human
A Abrahamsso, A Capodanno, A Rzepecka, C Dabrosin Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women Oncotarget, 2017;8(54):92134-92142. 2017 [PMID: 29190903] (Human) Human
Show All 67 Publications.

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Product General Protocols

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FAQs for IL-1ra/IL-1F3/IL1RN (DRA00B). (Showing 1 - 1 of 1 FAQs).

  1. wondering what the difference is between your quantikine and duo set elisas?
    • Usually the duosets do not have the entire kit such as plates and buffers, whereas the other kits are complete.

Other Available Formats

HRP DRA00B
HRP DRA00B
HRP DRA00B

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Bioinformatics

Gene Symbol IL1RN