Human IL-1ra/IL-1F3 Quantikine ELISA Kit

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Human IL-1ra/IL-1F3 Quantikine ELISA Kit Summary

The Quantikine Human IL-1ra Immunoassay is a 4.5 hour solid phase ELISA designed to measure IL-1ra in cell culture supernates, serum, and plasma. It contains E. coli-derived recombinant human IL-1ra as well as antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate recombinant human IL-1ra. Results obtained using natural human IL-1r...a showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for natural human IL-1ra.
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Natural and recombinant human IL-1ra
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Application Notes
Interference observed with 1 or more available related molecules.
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DRA00B in the following applications:

Packaging, Storage & Formulations

Store the unopened product at 2 - 8 °C. Do not use past expiration date.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Human IL-1ra/IL-1F3 Quantikine ELISA Kit

  • DIRA
  • ICIL-1ra
  • IL1F3
  • IL-1F3
  • IL1ra
  • IL-1ra
  • IL-1ra3
  • IL1RN
  • IL-1RN
  • interleukin 1 receptor antagonist
  • IRAP
  • MVCD4


Interleukin-1 receptor antagonist (IL-1ra; also known as IL-1F3) is a 22-25 kDa member of the IL-1 family of cytokines. Currently, there are 11 family members (IL-1F1-F11), nine of which form an IL-1 gene cluster on human Ch2 (1-3). Each IL-1 family member contains an IL-1 fold. This fold is generated by 12 packed beta -sheets that interact to form a beta -trefoil structure. Little amino acid (aa) homology is required to achieve this structure, and this explains the low aa identity among IL-1 family members. IL-1ra is a pure cytokine receptor antagonist that has no signal transduction-initiating activity (4). It is an acute phase protein that exists to dampen inflammation. IL-1( beta ) is initially produced by monocytes in response to a variety of stimuli. Circulating IL-1 then binds to widely expressed IL-1 type I receptors (IL-1 RI) and initiates a number of pro-inflammatory events. On endothelial cells (EC), IL-1 induces PGE2 and IL-6 release, generating fever, thrombocytosis, and hepatic acute phase protein production. In synovial joints, IL-1 induces chondrocyte NO production, an event that leads to reduced collagen synthesis and chondrocyte apoptosis. Finally, IL-1 increases neutrophil counts, both in blood and tissue, and thus is able to promote a pro-inflammatory environment in multiple locations (5-8). IL-1ra blocks IL-1 action through competitive inhibition. More correctly, although IL-1ra fills the IL-1 binding site in IL-1 RI, it is also unable to orchestrate the creation of a signal-transducing IL-1 RI:IL-1 R Accessory protein (IL-1 R AcP) heterodimer complex. Effective IL-1ra concentrations are generally 100-fold greater than local IL-1 concentrations. This is because the IL-1ra half-life is but 6 minutes, and very few IL-1 type I receptors need to be engaged by IL-1 to elicit a cellular response (5, 7, 9). 
Human IL-1ra is synthesized as a 177 aa precursor that contains a 25 aa signal sequence and a 152 aa mature region (10, 11). Although it contains an IL-1 cytokine fold, it apparently lacks two structural motifs that allow for activation of the IL-1 receptor heterodimer. First, and following binding to IL-1 RI, the presence of Ile 51-His 54 and Lys 145 of the mature molecule preclude recruitment of IL-1 R AcP. Second, there is no identifiable C-terminal lectin segment that is hypothesized to help recruit an accessory signaling component (1, 12, 13). Mature human IL-1ra is 77% and 82% aa identical to mouse and canine IL-1ra, respectively, and human IL-1ra inhibits IL-1 activity on mouse cells (10). A number of cell types express IL-1ra, including monocytes (11), Sertoli cells (14), hepatocytes (15), adipocytes (16), synovial fibroblasts (17), mast cells (18), pancreatic beta -cells (19), and intestinal epithelial cells (20). There are at least three intracellular IL-1ra isoforms (icIL-1ra1, 2, and 3). All show N-terminal variation, and all contain amino acids 35-177 of the secreted precursor (21-23). Intracellular IL-1ra1 is of particular interest, because it is reported to be "secreted" by endothelial cells and binds to the IL-1 RI in an antagonist fashion (23-25). Intracellular IL-1ra1 is 159 aa in length and shows a 3 aa substitution for the first 21 aa's of the signal sequence of IL-1ra (21).

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⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to

Publications for IL-1ra/IL-1F3/IL1RN (DRA00B)(94)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Ovine, Primate, Primate - Macaca mulatta (Rhesus Macaque), Primate - Papio hamadryas (Hamadyras Baboon).

We have publications tested in 1 application: ELISA Capture.

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Primate - Macaca mulatta (Rhesus Macaque)
Primate - Papio hamadryas (Hamadyras Baboon)
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Showing Publications 1 - 10 of 94. Show All 94 Publications.
Publications using DRA00B Applications Species
Burrows, K;Figueroa-Hall, L;Stewart, J;Alarbi, A;Kuplicki, R;Hannafon, B;Tan, C;Risbrough, V;McKinney, B;Ramesh, R;Victor, T;Aupperle, R;Savitz, J;Teague, K;Khalsa, S;Paulus, M; Exploring the role of neuronal-enriched extracellular vesicle miR-93 and interoception in major depressive disorder Research square 2023-06-16 [PMID: 37398092] (Human) Human
Buchheit, T;Huh, Y;Breglio, A;Bang, S;Xu, J;Matsuoka, Y;Guo, R;Bortsov, A;Reinecke, J;Wehling, P;Jun Huang, T;Ji, RR; Intrathecal Administration of Conditioned Serum from Different Species Resolves Chemotherapy-Induced Neuropathic Pain in Mice via Secretory Exosomes Brain, behavior, and immunity 2023-05-05 [PMID: 37150265] (Human) Human
LH Willems, LMC Jacobs, LA Groh, H Ten Cate, HMH Spronk, B Wilson-Sto, G Hannink, SMJ van Kuijk, C Ghossein-D, M Nagy, DHJ Thijssen, AS van Peters, MC Warlé Vascular Function, Systemic Inflammation, and Coagulation Activation 18 Months after COVID-19 Infection: An Observational Cohort Study Journal of Clinical Medicine, 2023-02-10;12(4):. 2023-02-10 [PMID: 36835948] (Human) Human
H Hallihan, P Tsai, N Lv, L Xiao, B Peñalver B, Y Wu, GN Pandey, LM Williams, OA Ajilore, J Ma Affective neural circuits and inflammatory markers linked to depression and anxiety symptoms in patients with comorbid obesity Journal of psychiatric research, 2023-02-03;160(0):9-18. 2023-02-03 [PMID: 36764197] (Human) Human
G Della Came, T Liu, W Yang, Y Li, VF Puntes, S Gioria, P Italiani, D Boraschi Induction of Innate Memory in Human Monocytes Exposed to Mixtures of Bacterial Agents and Nanoparticles International Journal of Molecular Sciences, 2022-11-24;23(23):. 2022-11-24 [PMID: 36498992] (Human) Human
D Chaar, BL Dumont, B Vulesevic, PE Neagoe, A Räkel, M White, MG Sirois Neutrophils and Circulating Inflammatory Biomarkers in Diabetes Mellitus and Heart Failure With Preserved Ejection Fraction The American journal of cardiology, 2022-07-08;0(0):. 2022-07-08 [PMID: 35811144] (Human) Human
DB Rosen, EA Murphy, RS Gejman, A Capili, RL Friedlande, S Rand, KA Cagino, SM Glynn, KC Matthews, JM Kubiak, J Yee, M Prabhu, LE Riley, YJ Yang Cytokine response over the course of COVID-19 infection in pregnant women Cytokine, 2022-04-25;154(0):155894. 2022-04-25 [PMID: 35490452] (Human) Human
D Martins, O Dipasquale, K Davies, E Cooper, J Tibble, M Veronese, M Frigo, SCR Williams, F Turkheimer, M Cercignani, NA Harrison Transcriptomic and cellular decoding of functional brain connectivity changes reveal regional brain vulnerability to pro- and anti-inflammatory therapies Brain, Behavior, and Immunity, 2022-03-05;102(0):312-323. 2022-03-05 [PMID: 35259429] (Human) Human
M Archer, O Niemelä, M Hämäläinen, E Moilanen, E Leinonen, O Kampman The role of alcohol use and adiposity in serum levels of IL-1RA in depressed patients BMC Psychiatry, 2022-03-02;22(1):158. 2022-03-02 [PMID: 35232419] (Human) Human
C Gar, B Thorand, C Herder, C Sujana, M Heier, C Meisinger, A Peters, W Koenig, W Rathmann, M Roden, M Stumvoll, H Maalmi, T Meitinger, H Then, J Seissler, C Then Association of circulating MR-proADM with all-cause and cardiovascular mortality in the general population: Results from the KORA F4 cohort study PLoS ONE, 2022-01-06;17(1):e0262330. 2022-01-06 [PMID: 34990470] (Human) Human
Show All 94 Publications.

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Gene Symbol IL1RN