Recombinant Human IL-1 alpha/IL-1F1 Protein


1 μg/lane of Recombinant Human IL-1 alpha /IL-1F1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.
Recombinant Human IL-1 alpha /IL-1F1 (Catalog # 200-LA) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 1-6 pg/mL.

Product Details

Reactivity HuSpecies Glossary
Applications Bioactivity

Order Details

View Available Formulations
Catalog# & Formulation Size Price

Recombinant Human IL-1 alpha/IL-1F1 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 1‑6 pg/mL.
E. coli-derived human IL-1 alpha/IL-1F1 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
200-LA in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 alpha/IL-1F1 Protein

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha


Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta, which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha. The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Customers Who Viewed This Item Also Viewed...

Species: Hu
Species: Hu
Applications: WB, Simple Western, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
Species: Hu
Applications: WB, Flow, ICC/IF
Species: Hu, Mu, Rt, Po, Sh
Applications: WB, Flow, ICC/IF, IHC, IHC-P, PEP-ELISA
Species: Hu
Applications: Bioactivity

Publications for IL-1 alpha/IL-1F1 (200-LA)(43)

We have publications tested in 5 confirmed species: Human, Mouse, Rat, Bovine, N/A.

We have publications tested in 5 applications: Bioassay, Control, ELISA (Standard), In Vivo, Western Blot.

Filter By Application
ELISA (Standard)
In Vivo
Western Blot
All Applications
Filter By Species
All Species
Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using 200-LA Applications Species
CJ Cunningham, R Wong, J Barrington, S Tamburrano, E Pinteaux, SM Allan Systemic conditioned medium treatment from interleukin-1 primed mesenchymal stem cells promotes recovery after stroke Stem Cell Res Ther, 2020;11(1):32. 2020 [PMID: 31964413] (Bioassay, Human) Bioassay Human
NE Powers, B Swartzwelt, C Marchetti, DM de Graaf, A Lerchner, M Schlapschy, R Datar, U Binder, CK Edwards, A Skerra, CA Dinarello PASylation of IL-1 Receptor antagonist (IL-1Ra) retains IL-1 blockade and extends its duration in mouse urate crystal-induced peritonitis J. Biol. Chem., 2019;0(0):. 2019 [PMID: 31819009] (Bioassay, Human) Bioassay Human
N Piippo, E Korhonen, M Hytti, K Kinnunen, K Kaarnirant, A Kauppinen Oxidative Stress is the Principal Contributor to Inflammasome Activation in Retinal Pigment Epithelium Cells with Defunct Proteasomes and Autophagy Cell. Physiol. Biochem., 2018;49(1):359-367. 2018 [PMID: 30138927] (Bioassay, Human) Bioassay Human
KC Ravindra, CC Ahrens, Y Wang, JY Ramseier, JS Wishnok, LG Griffith, AJ Grodzinsky, SR Tannenbaum Chemoproteomics of matrix metalloproteases in a model of cartilage degeneration suggests functional biomarkers associated with posttraumatic osteoarthritis J. Biol. Chem., 2018;0(0):. 2018 [PMID: 29794029] (Bioassay, Bovine) Bioassay Bovine
E Couderc, F Morel, P Levillain, A Buffière-M, M Camus, C Paquier, C Bodet, JF Jégou, M Pohin, L Favot, M Garcia, V Huguier, J Mcheik, C Lacombe, H Yssel, G Guillet, FX Bernard, JC Lecron Interleukin-17A-induced production of acute serum amyloid A by keratinocytes contributes to psoriasis pathogenesis PLoS ONE, 2017;12(7):e0181486. 2017 [PMID: 28708859] (Bioassay, Human) Bioassay Human
JH von Steman, AS Rigas, LW Thørner, DGK Rasmussen, OB Pedersen, K Rostgaard, C Erikstrup, H Ullum, MB Hansen Prevalence and correlation of cytokine-specific autoantibodies with epidemiological factors and C-reactive protein in 8,972 healthy individuals: Results from the Danish Blood Donor Study PLoS ONE, 2017;12(6):e0179981. 2017 [PMID: 28665954] (Control, Human) Control Human
B Tian, A Al-Moujahe, P Bouzika, Y Hu, S Notomi, P Tsoka, JW Miller, H Lin, DG Vavvas Atorvastatin Promotes Phagocytosis and Attenuates Pro-Inflammatory Response in Human Retinal Pigment Epithelial Cells Sci Rep, 2017;7(1):2329. 2017 [PMID: 28539592] (Bioassay, Human) Bioassay Human
E Redondo-Ca, C Cunningham, J Miller, L Martuscell, S Aoulad-Ali, NJ Rothwell, CM Kielty, SM Allan, E Pinteaux Interleukin-1 primes human mesenchymal stem cells towards an anti-inflammatory and pro-trophic phenotype in vitro Stem Cell Res Ther, 2017;8(1):79. 2017 [PMID: 28412968] (Bioassay, Human) Bioassay Human
KM Perrott, CD Wiley, PY Desprez, J Campisi Apigenin suppresses the senescence-associated secretory phenotype and paracrine effects on breast cancer cells Geroscience, 2017;39(2):161-173. 2017 [PMID: 28378188] (Bioassay, Human) Bioassay Human
Sojoong Choi Inflammatory hypoxia induces syndecan-2 expression through IL-1?-mediated FOXO3a activation in colonic epithelia FASEB J, 2016;0(0):. 2016 [PMID: 28031321] (Bioassay, Human) Bioassay Human
Show All 43 Publications.

Reviews for IL-1 alpha/IL-1F1 (200-LA) (0)

There are no reviews for IL-1 alpha/IL-1F1 (200-LA). By submitting a review you will receive an Amazon e-Gift Card or Novus Product Discount.
  • Review with no image -- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen
  • Review with an image -- $25/€18/£15/$25 CAD/¥150 Yuan/¥2500 Yen

FAQs for IL-1 alpha/IL-1F1 (200-LA) (0)

There are no specific FAQs related to this product. Read our general customer & technical service FAQs.

Other Available Formats

Additional IL-1 alpha/IL-1F1 Products

Bioinformatics Tool for IL-1 alpha/IL-1F1 (200-LA)

Discover related pathways, diseases and genes to IL-1 alpha/IL-1F1 (200-LA). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-1 alpha/IL-1F1 (200-LA)

Discover more about diseases related to IL-1 alpha/IL-1F1 (200-LA).

Pathways for IL-1 alpha/IL-1F1 (200-LA)

View related products by pathway.

PTMs for IL-1 alpha/IL-1F1 (200-LA)

Learn more about PTMs related to IL-1 alpha/IL-1F1 (200-LA).

Blogs on IL-1 alpha/IL-1F1

There are no specific blogs for IL-1 alpha/IL-1F1, but you can read our latest blog posts.

Customers Who Bought This Also Bought

Contact Information

Product PDFs


Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.


Review this Product

Be the first to review our Recombinant Human IL-1 alpha/IL-1F1 Protein and receive a gift card or discount.


Gene Symbol IL1A