Recombinant Human IL-1 alpha/IL-1F1 Protein

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Recombinant Human IL-1 alpha /IL-1F1 (Catalog # 200-LA) stimulates cell proliferation of the D10.G4.1 mouse helper T cell line. The ED50 for this effect is 1-6 pg/mL.
1 μg/lane of Recombinant Human IL-1 alpha /IL-1F1 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 18 kDa.

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Reactivity HuSpecies Glossary
Applications Bioactivity

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Recombinant Human IL-1 alpha/IL-1F1 Protein Summary

Details of Functionality
Measured in a cell proliferation assay using D10.G4.1 mouse helper T cells. Symons, J.A. et al. (1987) in Lymphokines and Interferons, a Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 272. The ED50 for this effect is 1‑6 pg/mL.
Source
E. coli-derived human IL-1 alpha/IL-1F1 protein
Ser113-Ala271
Accession #
N-terminal Sequence
Ser113
Protein/Peptide Type
Recombinant Proteins
Gene
IL1A
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.

Applications/Dilutions

Dilutions
  • Bioactivity
Theoretical MW
18 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Publications
Read Publications using
200-LA in the following applications:

Packaging, Storage & Formulations

Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Buffer
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Purity
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain
Reconstitution Instructions
Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IL-1 alpha/IL-1F1 Protein

  • BAF
  • Hematopoietin-1
  • IL1 alpha
  • IL-1 alpha
  • IL1
  • IL1A
  • IL-1A
  • IL1-ALPHA
  • IL1F1
  • IL-1F1
  • IL1F1hematopoietin-1
  • interleukin 1, alpha
  • interleukin-1 alpha
  • LAF
  • LEM
  • preinterleukin 1 alpha
  • pro-interleukin-1-alpha

Background

Interleukin 1 (IL-1) is a name that designates two proteins, IL-1 alpha and IL-1 beta , which are the products of distinct genes, but which show approximately 25% amino acid sequence identity and which recognize the same cell surface receptors. Although IL-1 production is generally considered to be a consequence of inflammation, recent evidence suggests that IL-1 is also temporarily upregulated during bone formation and the menstrual cycle and can be induced in response to nervous system stimulation. In response to classic stimuli produced by inflammatory agents, infections or microbial endotoxins, a dramatic increase in the production of IL-1 by macrophages and various other cells is seen. Cells in particular known to produce IL-1 include osteoblasts, monocytes, macrophages, keratinocytes, Kupffer cells, hepatocytes, thymic and salivary gland epithelium, Schwann cells, fibroblasts and glia (oligodendroglia, astrocytes and microglia).

IL-1 alpha and IL-1 beta are both synthesized as 31 kDa precursors that are subsequently cleaved into proteins with molecular weights of approximately 17,000 Da. Neither precursor contains a typical hydrophobic signal peptide sequence and most of the precursor form of IL-1 alpha remains in the cytosol of cells, although there is evidence for a membrane-bound form of the precursor form of IL-1 alpha . The IL-1 alpha precursor reportedly shows full biological activity in the EL-4 assay. Among various species, the amino acid sequence of mature IL-1 alpha is conserved 60% to 70% and human IL-1 has been found to be biologically active on murine cell lines. Both forms of IL-1 bind to the same receptors, designated type I and type II. Evidence suggests that only the type I receptor is capable of signal transduction and that the type II receptor may function as a decoy, binding IL-1 and thus preventing binding of IL-1 to the type I receptor.

Publications for IL-1 alpha/IL-1F1 (200-LA)(61)

We have publications tested in 4 confirmed species: Human, Mouse, Rat, Bovine.

We have publications tested in 6 applications: Bioassay, Cell Culture, Control, ELISA (Standard), In Vivo, Western Blot.


Filter By Application
Bioassay
(49)
Cell Culture
(2)
Control
(1)
ELISA (Standard)
(5)
In Vivo
(2)
Western Blot
(1)
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Human
(49)
Mouse
(3)
Rat
(1)
Bovine
(2)
All Species
Showing Publications 1 - 10 of 61. Show All 61 Publications.
Publications using 200-LA Applications Species
Farias-Jofre, M;Romero, R;Galaz, J;Xu, Y;Miller, D;Garcia-Flores, V;Arenas-Hernandez, M;Winters, AD;Berkowitz, BA;Podolsky, RH;Shen, Y;Kanninen, T;Panaitescu, B;Glazier, CR;Pique-Regi, R;Theis, KR;Gomez-Lopez, N; Blockade of IL-6R prevents preterm birth and adverse neonatal outcomes EBioMedicine 2023-11-07 [PMID: 37944273] (In Vivo, Mouse) In Vivo Mouse
Grabarczyk, M;Ksiazek-Winiarek, D;Glabinski, A;Szpakowski, P; Dietary Polyphenols Decrease Chemokine Release by Human Primary Astrocytes Responding to Pro-Inflammatory Cytokines Pharmaceutics 2023-09-07 [PMID: 37765263] (Bioassay, Human) Bioassay Human
Karema-Jokinen, V;Koskela, A;Hytti, M;Hongisto, H;Viheri�l�, T;Liukkonen, M;Torsti, T;Skottman, H;Kauppinen, A;Nymark, S;Kaarniranta, K; Crosstalk of protein clearance, inflammasome, and Ca2+ channels in retinal pigment epithelium derived from age-related macular degeneration patients The Journal of biological chemistry 2023-05-01 [PMID: 37137441] (Bioassay, Human) Bioassay Human
P Szpakowski, D Ksiazek-Wi, M Turniak-Ku, I Pacan, A Glabinski Human Primary Astrocytes Differently Respond to Pro- and Anti-Inflammatory Stimuli Biomedicines, 2022-07-22;10(8):. 2022-07-22 [PMID: 35892669] (Bioassay, Human) Bioassay Human
MC Ebeling, CR Fisher, RJ Kapphahn, MR Stahl, S Shen, J Qu, SR Montezuma, DA Ferrington Inflammasome Activation in Retinal Pigment Epithelium from Human Donors with Age-Related Macular Degeneration Cells, 2022-06-30;11(13):. 2022-06-30 [PMID: 35805159] (Bioassay, Human) Bioassay Human
E Filidou, L Kandilogia, G Tarapatzi, M Spathakis, P Steiropoul, D Mikroulis, K Arvanitidi, V Paspaliari, G Kolios Anti-Inflammatory and Anti-Fibrotic Effect of Immortalized Mesenchymal-Stem-Cell-Derived Conditioned Medium on Human Lung Myofibroblasts and Epithelial Cells International Journal of Molecular Sciences, 2022-04-20;23(9):. 2022-04-20 [PMID: 35562961] (Bioassay, Human) Bioassay Human
Y Zhu, LGPL Prata, EOW Gerdes, JME Netto, T Pirtskhala, N Giorgadze, U Tripathi, CL Inman, KO Johnson, A Xue, AK Palmer, T Chen, K Schaefer, JN Justice, AM Nambiar, N Musi, SB Kritchevsk, J Chen, S Khosla, D Jurk, MJ Schafer, T Tchkonia, JL Kirkland Orally-active, clinically-translatable senolytics restore alpha-Klotho in mice and humans EBioMedicine, 2022-03-13;0(0):103912. 2022-03-13 [PMID: 35292270] (Cell Culture, Human) Cell Culture Human
M McAleavy, Q Zhang, PJ Ehmann, J Xu, MF Wipperman, D Ajithdoss, L Pan, M Wakai, R Simonson, A Gadi, A Oyejide, SC Hamon, A Boyapati, LG Morton, T Shavlakadz, CA Kyratsous, DJ Glass The Activin/FLRG pathway associates with poor COVID-19 outcomes in hospitalized patients Molecular and Cellular Biology, 2021-11-01;0(0):MCB0046721. 2021-11-01 [PMID: 34723652] (Bioassay, Human) Bioassay Human
H Sheldon, J Alexander, E Bridges, L Moreira, S Reilly, KH Ang, D Wang, S Lin, S Haider, AH Banham, AL Harris ELTD1 Activation Induces an Endothelial-EMT Transition to a Myofibroblast Phenotype International Journal of Molecular Sciences, 2021-10-19;22(20):. 2021-10-19 [PMID: 34681953] (Bioassay, Human) Bioassay Human
R Biavasco, E Lettera, K Giannetti, D Gilioli, S Beretta, A Conti, S Scala, D Cesana, P Gallina, M Norelli, L Basso-Ricc, A Bondanza, G Cavalli, M Ponzoni, L Dagna, C Doglioni, A Aiuti, I Merelli, R Di Micco, E Montini Oncogene-induced senescence in hematopoietic progenitors features myeloid restricted hematopoiesis, chronic inflammation and histiocytosis Nature Communications, 2021-07-27;12(1):4559. 2021-07-27 [PMID: 34315896] (Bioassay, Human) Bioassay Human
Show All 61 Publications.

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Bioinformatics

Gene Symbol IL1A
Uniprot