IL-1 beta/IL-1F2 Antibody [Biotin]


Western Blot: IL-1 beta/IL-1F2 Antibody [Biotin] [NBP1-42768] - Lane 1: Mouse IL-1Beta Recombinant Protein. Load: 50 ng per lane. Primary antibody: Rabbit anti-Mouse IL-1Beta Antibody Biotin Conjugated at 1:1,000 more
Western Blot: IL-1 beta/IL-1F2 Antibody [Biotin] [NBP1-42768] - Lane 1: Mouse IL-1 beta Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.
Western Blot: IL-1 beta/IL-1F2 Antibody [Biotin] [NBP1-42768] - Lane 1: Mouse IL-1Beta Recombinant Protein. Load: 50 ng per lane. Primary antibody: Rabbit anti-Mouse IL-1Beta Antibody Biotin Conjugated at 1:1,000 more

Product Details

Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
LYOPH mg/ml

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Available Conjugates
Catalog# & Conjugate Size Price

IL-1 beta/IL-1F2 Antibody [Biotin] Summary

This antibody was prepared by repeated immunizations with recombinant mouse IL-1 beta produced in E.coli. The MW of recombinant mouse IL-1 beta was 17 kDa.
This antibody is primarily directed against mature, 17,000 MW mouse IL-1 beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1 beta or mouse IL-1a. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1 beta. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody.
Ion exchange chromatography
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Packaging, Storage & Formulations

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Biotin (A=N/A, E=N/A)
0.02 M Potassium Phosphate (pH 7.2), and 0.15 M NaCl
0.01% Sodium Azide
LYOPH mg/ml
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.


Application Notes
This antibody recognizes the 17,000 MW mature IL-1Beta. For immunoblots, typically, IL-1Beta is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells. PBMC are stimulated for 24 hours with 1% serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG - reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation - stain intracellular IL-1Beta. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of mouse and rat IL-1Beta activity in bioassays. It does not neutralize the biological activity IL-1alpha. It does not neutralize the biological activity of human or primate IL-1Beta. For neutralization, it is recommended - incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used f. Caution should be exhibited as the F domain of the rabbit IgG molecule may interact with cells non-specifically.Flow Cytometry was reported in scientific literature.
Theoretical MW
17 kDa
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NBP1-42768 in the following applications:

Alternate Names for IL-1 beta/IL-1F2 Antibody [Biotin]

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1B
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta


IL-1 beta (also known as Interleukin-1 beta, IL-1beta and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IL-1 beta/IL-1F2 Antibody (NBP1-42768)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.

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Product General Protocols

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WB Video Protocol

FAQs for IL-1 beta/IL-1F2 Antibody (NBP1-42768). (Showing 1 - 1 of 1 FAQ).

  1. I bought the product NBP1-42768 and I'm not sure about the reconstitution and the concentration. I have 100ug, so if I add 50ul, I will have a concentration of 2mg/ml, right?
    • Centrifuge vial prior to adding liquid. Add 100ul of deionized water and resuspend. The final concentration will be 1mg/ml.

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Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

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Gene Symbol IL1B

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