IL-1 beta/IL-1F2 Antibody [Biotin]

Images

 
Western Blot: IL-1 beta/IL-1F2 Antibody [Biotin] [NBP1-42768] - Lane 1: Mouse IL-1Beta Recombinant Protein. Load: 50 ng per lane. Primary antibody: Rabbit anti-Mouse IL-1Beta Antibody Biotin Conjugated at 1:1,000 ...read more
Western Blot: IL-1 beta/IL-1F2 Antibody [Biotin] [NBP1-42768] - Lane 1: Mouse IL-1 beta Recombinant Protein. Load: 50 ng per lane. Used at a dilution of 1:1,000 overnight at 4C.
Western Blot: IL-1 beta/IL-1F2 Antibody [Biotin] [NBP1-42768] - Lane 1: Mouse IL-1Beta Recombinant Protein. Load: 50 ng per lane. Primary antibody: Rabbit anti-Mouse IL-1Beta Antibody Biotin Conjugated at 1:1,000 ...read more

Product Details

Summary
Reactivity Mu, RtSpecies Glossary
Applications WB, ELISA, Flow, IHC, IHC-P
Clonality
Polyclonal
Host
Rabbit
Conjugate
Biotin
Concentration
LYOPH

Order Details

View Available Conjugates
Catalog# & Conjugate Size Price

IL-1 beta/IL-1F2 Antibody [Biotin] Summary

Immunogen
This antibody was prepared by repeated immunizations with recombinant mouse IL-1 beta produced in E.coli. The MW of recombinant mouse IL-1 beta was 17 kDa.
Specificity
This antibody is primarily directed against mature, 17,000 MW mouse IL-1 beta and is useful in determining its presence in various assays. The antibody does not recognize human IL-1 beta or mouse IL-1a. In ELISA formats and other immunoreactive assays, reactivity occurs with rat IL-1 beta. This antibody will recognize 10% of the non-denatured (native) precursor 31,000 MW mouse IL-1 beta containing samples but will primarily detect all of the 17,000 MW mature molecule. However, in immunoblot analysis, the usual procedure of heating the sample in SDS with or without reducing agents will facilitate denaturing of the 31,000 MW IL- 1 beta precursor molecule. Denatured 31,000 precursor IL-1 beta will be recognized by this antibody.
Isotype
IgG
Clonality
Polyclonal
Host
Rabbit
Gene
IL1B
Purity
Ion exchange chromatography
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.

Applications/Dilutions

Dilutions
  • Western Blot 1:2000 - 1:10000
  • ELISA 1:20000-1:100000
  • Flow Cytometry 1:10-1:1000
  • Immunohistochemistry 1:1000-1:5000
  • Immunohistochemistry-Paraffin 1:10-1:500
Application Notes
This antibody recognizes the 17,000 MW mature IL-1Beta. For immunoblots, typically, IL-1Beta is detected from supernatants or lysates of 2 x 10E6 endotoxin-stimulated peripheral blood mononuclear cells. PBMC are stimulated for 24 hours with 1% serum plus 10 ng/mL E.coli LPS. For immunoprecipitation pre-clearing the preparation with a non-specific Rabbit IgG - reduce background is suggested. For immunohistochemistry either paraffin fixation or cryofixation can be used for sample preparation - stain intracellular IL-1Beta. For ELISA use HRP Conjugated Anti-Rabbit IgG [H&L] for detection. In ELISA formats this antibody is best used as the second antibody in combination with a monoclonal antibody as a capture antibody. This antibody is also useful for neutralization of mouse and rat IL-1Beta activity in bioassays. It does not neutralize the biological activity IL-1alpha. It does not neutralize the biological activity of human or primate IL-1Beta. For neutralization, it is recommended - incubate the sample with a dilution of the antibody for at least 4 hours before being tested. A control of similarly diluted normal rabbit IgG is recommended. This antibody can be used f. Caution should be exhibited as the F domain of the rabbit IgG molecule may interact with cells non-specifically.Flow Cytometry was reported in scientific literature.
Theoretical MW
17 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Readout System
Reviewed Applications
Read 1 Review rated 5
using
NBP1-42768 in the following applications:

Publications
Read Publication using
NBP1-42768 in the following applications:

Packaging, Storage & Formulations

Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Buffer
0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2, 10 mg/mL Bovine Serum Albumin (BSA) - Immunoglobulin and Protease free
Preservative
0.01% Sodium Azide
Concentration
LYOPH
Purity
Ion exchange chromatography
Reconstitution Instructions
Reconstitute with 0.1 ml deionized water.

Alternate Names for IL-1 beta/IL-1F2 Antibody [Biotin]

  • catabolin
  • IL1 beta
  • IL-1 beta
  • IL-1
  • IL1B
  • IL-1b
  • IL1-BETA
  • IL-1F2
  • IL1F2IL-1 beta
  • interleukin 1, beta
  • interleukin-1 beta
  • preinterleukin 1 beta
  • pro-interleukin-1-beta

Background

IL-1 beta (also known as Interleukin-1 beta, IL-1beta and catabolin) is produced by activated macrophages. IL-1 stimulates thymocyte proliferation by inducing IL-2 release, B-cell maturation and proliferation, and fibroblast growth factor activity. IL-1 proteins are involved in the inflammatory response, being identified as endogenous pyrogens, and are reported to stimulate the release of prostaglandin and collagenase from synovial cells. IL-1beta is a monomeric secreted protein that may be released by damaged cells or is secreted by a mechanism differing from that used for other secretory proteins.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customers Who Viewed This Item Also Viewed...

MAB208
Species: Hu
Applications: WB, CyTOF-ready, ELISA(Cap), ELISA(Det), ICC, ICFlow, Neut, ELISA(Sta)
NB300-605
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
NBP2-29323
Species: Hu, Mu, Rt, Bv, Ca, Ch, Xp
NBP1-89522
Species: Hu, Mu, Rt
Applications: WB, ICC/IF, IHC, IHC-P
796-IC
Species: Mu
NBP1-42768
Species: Mu, Rt
Applications: WB, ELISA, Flow, IHC, IHC-P

Publications for IL-1 beta/IL-1F2 Antibody (NBP1-42768)(1)

We have publications tested in 1 confirmed species: Mouse.

We have publications tested in 1 application: FLOW.


Filter By Application
FLOW
(1)
All Applications
Filter By Species
Mouse
(1)
All Species

Review for IL-1 beta/IL-1F2 Antibody (NBP1-42768) (1) 51

Average Rating: 5
(Based on 1 review)
We have 1 review tested in 1 species: Mouse.
We have 1 review tested in 1 application: Flow.

Reviews using NBP1-42768:
Filter by Applications
Flow
(1)
All Applications
Filter by Species
Mouse
(1)
All Species
Images Ratings Applications Species Date Details
Flow Cytometry IL-1 beta/IL-1F2 NBP1-42768
Enlarge
reviewed by:
Jim Hsiao
Flow Mouse 02/27/2017
View

Summary

ApplicationFlow Cytometry
Sample TestedBone marrod derived cells
SpeciesMouse
Lot26217

Comments

CommentsSingle cell suspension was blocked with Fc block for 30 minutes, prior to the permeabilization with BD cytoperm/fix kit for an additional of 30 minutes. Cells were then washed and incubated with the biotinylated-conjugated IL1b antibody (1:100 in PERM buffer) for 30 minutes on ice. Cells were then briefly washed and analyzed by flow cytometry.

Product General Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

Video Protocols

WB Video Protocol

FAQs for IL-1 beta/IL-1F2 Antibody (NBP1-42768). (Showing 1 - 1 of 1 FAQ).

  1. I bought the product NBP1-42768 and I'm not sure about the reconstitution and the concentration. I have 100ug, so if I add 50ul, I will have a concentration of 2mg/ml, right?
    • Centrifuge vial prior to adding liquid. Add 100ul of deionized water and resuspend. The final concentration will be 1mg/ml.

Secondary Antibodies

 

Isotype Controls

Additional IL-1 beta/IL-1F2 Products

Bioinformatics Tool for IL-1 beta/IL-1F2 Antibody (NBP1-42768)

Discover related pathways, diseases and genes to IL-1 beta/IL-1F2 Antibody (NBP1-42768). Need help? Read the Bioinformatics Tool Guide for instructions on using this tool.
Visit Tool

Diseases for IL-1 beta/IL-1F2 Antibody (NBP1-42768)

Discover more about diseases related to IL-1 beta/IL-1F2 Antibody (NBP1-42768).
 

Pathways for IL-1 beta/IL-1F2 Antibody (NBP1-42768)

View related products by pathway.

PTMs for IL-1 beta/IL-1F2 Antibody (NBP1-42768)

Learn more about PTMs related to IL-1 beta/IL-1F2 Antibody (NBP1-42768).
 

Research Areas for IL-1 beta/IL-1F2 Antibody (NBP1-42768)

Find related products by research area.

Blogs on IL-1 beta/IL-1F2.

IL-1 beta (interleukin 1 beta, lymphocyte activating factor (LAF))
IL-1 was originally identified and cloned as a lymphocyte mitogen and much later, was found to be comprised of two closely related but distinct proteins, interleukin 1 alpha (IL-1 alpha) and interleukin 1 beta (IL-1 beta). Both these proteins bind...  Read full blog post.

Customers Who Bought This Also Bought

Contact Information

Product PDFs

Calculators

Concentration Calculator

The concentration calculator allows you to quickly calculate the volume, mass or concentration of your vial. Simply enter your mass, volume, or concentration values for your reagent and the calculator will determine the rest.

=
÷

Recent Reviews

5
5
1
4
0
3
0
2
0
1
0

Jim Hsiao
02/27/2017
Application: Flow
Species: Mouse

Bioinformatics

Gene Symbol IL1B
Entrez