IGF-II R/IGF2R Antibody

Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR was performed by loading various amounts of HeLa and Jurkat whole cell lysates per lane.

Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm and nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left).

Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).

Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).

• Reactivity: Hu, Mu, Bv, Pl, Pm
• Applications: WB, DB, ICC/IF, NULL
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

IGF-II R/IGF2R Antibody Summary

• Description: Novus Biologicals Rabbit IGF-II R/IGF2R Antibody (NB100-74387) is a polyclonal antibody validated for use in WB and ICC/IF. Anti-IGF-II R/IGF2R Antibody: Cited in 1 publication. All Novus Biologicals antibodies are covered by our 100% guarantee.
• Immunogen: 300 kDa cation-independent receptor purified from adult bovine liver tissue.
• Marker: Late Endosome Marker
• Specificity: CI-MPR
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: IGF2R
• Purity: Unpurified

Applications/Dilutions

• Dilutions:
  • Dot Blot 1:1000
  • Immunocytochemistry/ Immunofluorescence 1:50 - 1:500
  • Tissue Culture Substratum
  • Western Blot 1:2000 - 1:3000
• Application Notes: In WB: Detects a 300 kDa protein representing Cl-MPR. May be useful in IP.

Reactivity Notes

Plant reactivity reported in scientific literature (PMID: 26053072).

Packaging, Storage & Formulations

• Storage: Store at -20C. Avoid freeze-thaw cycles.
• Buffer: Whole antisera
• Preservative: 0.05% Sodium Azide
• Purity: Unpurified

Alternate Names for IGF-II R/IGF2R Antibody

• 300 kDa mannose 6-phosphate receptor
• cation-independent mannose-6 phosphate receptor
• cation-independent mannose-6-phosphate receptor
• CD222 antigen
• CD222
• CI Man-6-P receptor
• CIMPR
• CI-MPR
• IGF2R
• IGF-II R
• IGF-II receptor
• IGFIIR
• IGF-IIR
• insulin-like growth factor 2 receptorM6P/IGF2R
• Insulin-like growth factor II receptor
• M6P/IGF2 receptor
• M6PR
• M6P-R
• MPR 300
• MPR1
• MPRI
• MPRIM6PR

Background

The Cation independent Mannose 6 Phosphate Receptor transports phosphorylated lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. Lysosomal enzymes bearing phosphomannosyl residues bind specifically to mannose 6 phosphate receptors in the Golgi apparatus and the resulting receptor ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. This receptor also binds IGF2.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for IGF-II R/IGF2R Antibody (NB100-74387)(1)

Publication using NB100-74387

• Su J, Sherman A, Doerfler PA et al. Oral delivery of Acid Alpha Glucosidase epitopes expressed in plant chloroplasts suppresses antibody formation in treatment of Pompe mice Plant Biotechnol. J. 2015-06-05 [PMID: 26053072] (WB, Plant)

FAQs for IGF-II R/IGF2R Antibody (NB100-74387). (Showing 1 - 1 of 1 FAQ).

1. What is your modified RIPA buffer recipe?
   • 25mM Tris-HCl (pH 7.6), 150mM NaCl, 1% NP-40, 1% sodium deoxycholate, 0.1% SDS

Bioinformatics

• Gene Symbol: IGF2R
• Entrez:
  • 281849(Bovine)
  • 3482(Human)
  • 16004(Mouse)
• Uniprot:
  • P08169(Bovine)