After August 17, 2026, Novus Biologicals products and services will no longer be available on this website; you will access all products and services on rndsystems.com. Create your R&D Systems online account today.
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_lightbox" target="_blank">
NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_lightbox" target="_blank">
MAB24471) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGF-II R/IGF2R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2447) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
CD222 is a 250kDa transmembrane protein with a short cytoplasmic tail containing an internalization signal. CD222 was originally identified as a receptor for IGFII and M6P-containing proteins (e.g. lysosomal hydrolases). Lysosomal enzymes are sorted to lysosomes via CD222 either from the Golgi, where the enzymes acquire M6P, or from the extracellular space. The majority of CD222 molecules (approximately 90-95%) are located intracellularly, only 5-10% is present on the cell membrane. The internalization rate seems to be enhanced by ligand induced dimerization of CD222 as well as by phosphorylation of its cytoplasmic serine. CD222 is also a receptor for TGFbeta latency associated peptide (LAP), proliferin and may bind several molecules independently of M6P, including plasminogen, CD87 or retinoic acid. It is involved in activation of latent TGFbeta [PROW].
![Flow Cytometry IGF-II R/IGF2R Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IGF-II_R_AF2447_Flow_Cytometry_8177.jpg)
![N/A IGF-II R/IGF2R [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IGFII_R_DY2447_ELISA_1831.jpg)
![Immunocytochemistry IGF-II R/IGF2R Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IGFII_R_AF2447_Immunocytochemistry__Immunofluorescence_24176.jpg)
![Immunocytochemistry IGF-II R/IGF2R Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IGFII_R_AF2447_Immunocytochemistry__Immunofluorescence_24177.jpg)
![Immunohistochemistry IGF-II R/IGF2R Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/IGF-II_R_AF2447_Immunohistochemistry_6750.jpg)
![Western Blot IGF-II R/IGF2R Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af2447_human-igf-ii-r-affinity-purified-polyclonal-ab-western-blot-12122025549917.jpg)
![ELISA IGF-II R/IGF2R Antibody [Unconjugated]](http://images.novusbio.com/images/af2447_human-igf-ii-r-igf2r-affinity-purified-polyclonal-ab-elisa-639201852917443929.jpg)
![Binding Activity IGF-II R/IGF2R [Unconjugated]](http://images.novusbio.com/images/11782-gr_recombinant-human-igf-ii-r-igf2r-fc-chimera-protein-cf-binding-activity-2332026173522.jpg)
![SDS-Page IGF-II R/IGF2R [Unconjugated]](http://images.novusbio.com/fullsize/11782-gr_recombinant-human-igf-ii-r-igf2r-fc-chimera-protein-cf-sds-page-2332026175553.jpg)
![Binding Activity IGF-II R/IGF2R [Unconjugated]](http://images.novusbio.com/images/11783-gr_recombinant-human-igf-ii-r-igf2r-his-tag-protein-cf-binding-activity-303202615554.jpg)
![SDS-Page IGF-II R/IGF2R [Unconjugated]](http://images.novusbio.com/fullsize/11783-gr_recombinant-human-igf-ii-r-igf2r-his-tag-protein-cf-sds-page-303202615140.jpg)
