p57 Kip2 Antibody

Immunohistochemistry-Paraffin: p57 Kip2 Antibody [NBP1-89917] - Staining in human placenta and colon tissues using anti-CDKN1C antibody. Corresponding CDKN1C RNA-seq data are presented for the same tissues.

Western Blot: p57 Kip2 Antibody [NBP1-89917] - Analysis in human placenta tissue.

Immunocytochemistry/Immunofluorescence: p57 Kip2 Antibody [NBP1-89917] - Staining of human cell line U-2 OS shows localization to nucleoplasm, nuclear bodies & cytosol.

Immunohistochemistry-Paraffin: p57 Kip2 Antibody [NBP1-89917] - Staining of human colon shows low expression as expected.

Immunohistochemistry-Paraffin: p57 Kip2 Antibody [NBP1-89917] - Staining of human placenta shows high expression.

• Reactivity: Hu
• Applications: WB, ICC/IF, IHC
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

p57 Kip2 Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: TMERLVARGTFPVLVRTSACRSLFGPVDHEELSRELQARLAELNAEDQNRWDYDFQQDMPLRGPGRLQWTEVDSDSVPAFYRETVQVGRCRLLLAPRPVAVAVAVSPPLEPAAESLDGLEEAPEQLPSVPVP
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: CDKN1C
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Immunohistochemistry 1:50 - 1:200
  • Immunohistochemistry-Paraffin 1:50 - 1:200
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.
• Control Peptide:

  p57 Kip2 Protein (NBP1-89917PEP)

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for p57 Kip2 Antibody

• Beckwith-Wiedemann syndrome
• BWS
• cyclin-dependent kinase inhibitor 1C (p57, Kip2)
• cyclin-dependent kinase inhibitor 1C
• Cyclin-dependent kinase inhibitor p57
• KIP2BWCR
• p57
• p57Kip2
• WBS

Background

p57kip2 is a tight-binding, strong inhibitor of several G1 cyclin/Cdk complexes and a negative regulator of cell proliferation. Mutations in this gene are implicated in sporadic cancers and Beckwith-Wiedemann syndorome, suggesting that this gene is a tumor suppressor candidate.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for p57 Kip2 Antibody (NBP1-89917)(1)

Publication using NBP1-89917

• Mochizuki M, Tamai K, Imai T et al. CD271 regulates the proliferation and motility of hypopharyngeal cancer cells. Sci Rep. 2016-07-29 [PMID: 27469492] (WB, Human)

Bioinformatics

• Gene Symbol: CDKN1C