IGF-I R/IGF1R Antibody (33255) [Unconjugated]


Western blot shows lysates of NTera‑2 human testicular embryonic carcinoma cell line, SK‑Mel‑28 human malignant melanoma cell line, and G361 human melanoma cell line. PVDF membrane was probed with 1 µg/mL of ...read more
MCF‑7 human breast cancer cell line was stained with Mouse Anti-Human IGF‑I R/IGF1R Monoclonal Antibody (Catalog # MAB391, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram) ...read more
IGF-I R/IGF1R was detected in immersion fixed MCF‑7 human breast cancer cell line (positive staining; left panel) and HDLM human Hodgkin's lymphoma cell line (negative staining; right panel) using Mouse ...read more
IGF-I R/IGF1R was detected in perfusion fixed paraffin-embedded sections of mouse heart using Mouse Anti-Human/Mouse IGF-I R/IGF1R Monoclonal Antibody (Catalog # MAB391) at 15 µg/mL for 1 hour at room temperature ...read more
Recombinant Human (rh) IGF‑I/IGF-1 (Catalog # 291-G1) stimulates proliferation in the MCF‑7 human breast cancer cell line in a dose-dependent manner (orange line). Proliferation elicited by rhIGF‑I/IGF-1 (6 ng/mL) ...read more

Product Details

Reactivity Hu, MuSpecies Glossary
Applications WB, Flow, IHC, B/N, ELISA(Cap), ICC/IF

Order Details

IGF-I R/IGF1R Antibody (33255) [Unconjugated] Summary

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IGF-I R/IGF1R
Accession # P08069
Detects human IGF-I R/IGF1R in sandwich ELISAs and Western blots. Detects mouse IGF-I R/IGF1R in Immunohistochemistry. In sandwich immunoassays, less than 0.15% cross-reactivity or interference was observed with recombinant human (rh) IGF-I, rhIGF‑II, rhIL‑3 R alpha , rhIL‑9 R, and rhTGF‑ beta  RII.
Purity Statement
Protein A or G purified from hybridoma culture supernatant
Endotoxin Note
<0.10 EU per 1 μg of the antibody by the LAL method.
Innovator's Reward
Test in a species/application not listed above to receive a full credit towards a future purchase.


  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Flow Cytometry 0.25 ug/10^6 cells
  • Immunocytochemistry 3-25 ug/mL
  • Immunohistochemistry 5-25 ug/mL
  • Neutralization
  • Western Blot 1 ug/mL
Application Notes
ELISA Detection: Human IGF-I R Biotinylated Antibody (Catalog number BAF391)
Standard: Recombinant Human IGF-I R (Catalog number 391-GR)
Read Publications using
MAB391 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
No Preservative
Reconstitution Instructions
Reconstitute at 0.5 mg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for IGF-I R/IGF1R Antibody (33255) [Unconjugated]

  • CD221 antigen
  • CD221
  • EC 2.7.10
  • EC
  • IGF1R
  • IGF-1R
  • IGF-I R
  • IGF-I receptor
  • IGF-IR
  • IGFR
  • insulin-like growth factor 1 receptor
  • Insulin-like growth factor I receptor
  • JTK13
  • MGC142170
  • MGC142172
  • MGC18216
  • soluble IGF1R variant 1
  • soluble IGF1R variant 2


Insulin-like growth factor I receptor (IGF-I R) is a disulfide-linked heterotetrameric transmembrane protein consisting of two alpha and two beta subunits. Both the alpha and beta subunits are encoded within a single receptor precursor cDNA. The proreceptor polypeptide is proteolytically cleaved and disulfide-linked to yield the mature heterotetrameric receptor. The alpha subunit of IGF-I R is extracellular while the beta subunit has an extracellular domain, a transmembrane domain and a cytoplasmic tyrosine kinase domain. IGF-I R is highly expressed in all cell types and tissues. Essentially all of the biological activities of IGF-I and -II have been shown to be mediated via IGF-I R.


This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Publications for IGF-I R/IGF1R Antibody (MAB391)(18)

We have publications tested in 3 confirmed species: Human, Mouse, Bovine.

We have publications tested in 7 applications: ELISA Capture, ELISA Development, Flow Cytometry, ICC, IHC-P, Neutralization, Western Blot.

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ELISA Development
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Showing Publications 1 - 10 of 18. Show All 18 Publications.
Publications using MAB391 Applications Species
Miyagawa, K;Tenshin, H;Mulcrone, PL;Delgado-Calle, J;Subler, MA;Windle, JJ;Chirgwin, JM;Roodman, GD;Kurihara, N; Osteoclast-derived IGF1 induces RANKL production in osteocytes and contributes to Pagetic lesion formation JCI insight 2023-06-20 [PMID: 37338990] (Neutralization, Mouse) Neutralization Mouse
T Khan, AM Seddon, AG Dalgleish, S Khelwatty, N Ioannou, S Mudan, H Modjtahedi Synergistic activity of agents targeting growth factor receptors, CDKs and downstream signaling molecules in a panel of pancreatic cancer cell lines and the identification of antagonistic combinations: Implications for future clinical trials in pancreatic cancer Oncol Rep, 2020-10-22;0(0):. 2020-10-22 [PMID: 33125153] (Flow Cytometry, Human) Flow Cytometry Human
J Li, E Weber, S Guth-Gunde, M Schuleit, A Kuttler, C Halleux, N Accart, A Doelemeyer, A Basler, B Tigani, K Wuersch, M Fornaro, M Kneissel, A Stafford, BR Freedman, DJ Mooney Tough Composite Hydrogels with High Loading and Local Release of Biological Drugs Adv Healthc Mater, 2018-02-14;0(0):. 2018-02-14 [PMID: 29441702] (ELISA Capture, Mouse) ELISA Capture Mouse
Y Neuzillet, E Chapeaubla, C Krucker, L De Koning, T Lebret, F Radvanyi, I Bernard-Pi IGF1R activation and the in vitro antiproliferative efficacy of IGF1R inhibitor are inversely correlated with IGFBP5 expression in bladder cancer BMC Cancer, 2017-09-07;17(1):636. 2017-09-07 [PMID: 28882129] (Neutralization, Human) Neutralization Human
Helmout Modjtahedi Impact of the putative cancer stem cell markers and growth factor receptor expression on the sensitivity of ovarian cancer cells to treatment with various forms of small molecule tyrosine kinase inhibitors and cytotoxic drugs Int. J. Oncol., 2016-09-05;49(5):1825-1838. 2016-09-05 [PMID: 27599579] (Flow Cytometry, Western Blot, Human) Flow Cytometry, Western Blot Human
M Gschweitl, A Ulbricht, CA Barnes, RI Enchev, I Stoffel-St, N Meyer-Scha, J Huotari, Y Yamauchi, UF Greber, A Helenius, M Peter A SPOPL/Cullin-3 ubiquitin ligase complex regulates endocytic trafficking by targeting EPS15 at endosomes Elife, 2016-03-23;5(0):. 2016-03-23 [PMID: 27008177] (Western Blot, Human) Western Blot Human
Garwood C, Ratcliffe L, Morgan S, Simpson J, Owens H, Vazquez-Villasenor I, Heath P, Romero I, Ince P, Wharton S Insulin and IGF1 signalling pathways in human astrocytes in vitro and in vivo; characterisation, subcellular localisation and modulation of the receptors. Mol Brain, 2015-08-22;8(0):51. 2015-08-22 [PMID: 26297026] (Neutralization, Human) Neutralization Human
Cardillo T, Trisal P, Arrojo R, Goldenberg D, Chang C Targeting both IGF-1R and mTOR synergistically inhibits growth of renal cell carcinoma in vitro. BMC Cancer, 2013-04-01;13(0):170. 2013-04-01 [PMID: 23548153] (Flow Cytometry, Human) Flow Cytometry Human
Suh H, Zhao M, Derico L, Choi N, Lee S Insulin-like growth factor 1 and 2 (IGF1, IGF2) expression in human microglia: differential regulation by inflammatory mediators. J Neuroinflammation, 2013-03-12;10(0):37. 2013-03-12 [PMID: 23497056] (ICC, Human) ICC Human
Jacques C, Holzenberger M, Mladenovic Z Proinflammatory actions of visfatin/nicotinamide phosphoribosyltransferase (Nampt) involve regulation of insulin signaling pathway and Nampt enzymatic activity. J. Biol. Chem., 2012-03-07;287(18):15100-8. 2012-03-07 [PMID: 22399297] (Neutralization, Human) Neutralization Human
Show All 18 Publications.

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Gene Symbol IGF1R