MEST Antibody

Western Blot: MEST Antibody [NBP1-87299] - Analysis in control (vector only transfected HEK293T lysate) and MEST over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells, LY419353).

Immunocytochemistry/Immunofluorescence: MEST Antibody [NBP1-87299] - Immunofluorescent staining of human cell line A-431 shows positivity in golgi apparatus. Antibody staining in green.

• Reactivity: Hu, Mu
• Applications: WB, ICC/IF
• Clonality: Polyclonal
• Host: Rabbit
• Conjugate: Unconjugated

MEST Antibody Summary

• Immunogen: This antibody was developed against Recombinant Protein corresponding to amino acids: HSWKSSGKFFTYKGLRIFYQDSVGVVGSPEIVVLLHGFPTSSYDWYKIWEGLTLRFHRVIALDFLGFGFSDKPRPHHYSIFEQASIVEALLRHLGLQNRRINLLSHDYGDIVAQELLYRYKQNRSGRLTIKSLCLSNGGIFPETHRPL
• Predicted Species: Mouse (98%). Backed by our 100% Guarantee.
• Isotype: IgG
• Clonality: Polyclonal
• Host: Rabbit
• Gene: MEST
• Purity: Immunogen affinity purified

Applications/Dilutions

• Dilutions:
  • Immunocytochemistry/Immunofluorescence 0.25-2 ug/ml
  • Western Blot 0.04-0.4 ug/ml
• Application Notes: ICC/IF, Fixation Permeabilization: Use PFA/Triton X-100.

Reactivity Notes

Rat (89%).

Packaging, Storage & Formulations

• Storage: Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
• Buffer: PBS (pH 7.2) and 40% Glycerol
• Preservative: 0.02% Sodium Azide
• Purity: Immunogen affinity purified

Alternate Names for MEST Antibody

• DKFZp686L18234
• EC 3.-
• mesoderm specific transcript (mouse) homolog
• mesoderm specific transcript homolog (mouse)
• mesoderm-specific transcript homolog protein
• MGC111102
• MGC8703
• paternally expressed gene 1
• PEG1Paternally-expressed gene 1 protein

Background

MEST encodes a member of the [alpha]/[beta] hydrolase fold family and has isoform specific imprinting. The loss of imprinting of this gene has been linked to certain types of cancer and may be due to promotor switching. The encoded protein may play a role in development. Three transcript variants encoding two distinct isoforms have been identified for this gene. A pseudogene for this locus is located on chromosome 6. [provided by RefSeq]

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for MEST Antibody (NBP1-87299)(1)

Publication using NBP1-87299

• Itoh M, Tahimic CG, Ide S et al. Methyl CpG-binding protein isoform MeCP2_e2 is dispensable for Rett syndrome phenotypes but essential for embryo viability and placenta development. J Biol Chem 2012-04-01 [PMID: 22375006]

Bioinformatics

• Gene Symbol: MEST