Recombinant Human IGF-II/IGF2 Protein, CF


Recombinant Human IGF-II/IGF2 (Catalog # 292‑G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 1.5‑6 ng/mL.
1 μg/lane of Recombinant Human IGF‑II/IGF2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.

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Recombinant Human IGF-II/IGF2 Protein, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 1.5‑6 ng/mL.
E. coli-derived human IGF-II/IGF2 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


  • Bioactivity
Theoretical MW
7.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
292-G2 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-II/IGF2 Protein, CF

  • C11orf43
  • chromosome 11 open reading frame 43
  • FLJ22066
  • FLJ44734
  • GRDF
  • IGF2
  • IGF-2
  • IGF-II
  • insulin-like growth factor 2 (somatomedin A)
  • insulin-like growth factor II
  • insulin-like growth factor type 2
  • MSA
  • PEG2
  • PP9974
  • somatomedin-A


Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine and porcine proteins) and exhibit cross-species activity.

IGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development.

Two specific cell surface receptors that bind IGF-I and IGF-II have been identified. The type I IGF receptor that participates in IGF signaling is structurally related to the insulin receptor. It is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain. Type I IGF receptor binds IGF-I with higher affinity than IGF-II. The type II IGF receptor which binds IGF-II with much higher affinity than IGF-I is also the cation-independent mannose 6-phosphate receptor. At the present time, it is not known if the type II IGF receptor participates in the IGF signaling pathway. An additional unknown receptor which mediates IGF‑II signaling has also been proposed. Circulating IGFs exist in complexes bound to IGF binding proteins. Currently, at least six high affinity binding proteins have been identified.

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Publications for IGF-II/IGF2 (292-G2)(43)

We have publications tested in 7 confirmed species: Human, Mouse, Rat, Bovine, Chicken, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque).

We have publications tested in 10 applications: Binding Assay, Bioassay, Cell Culture, ELISA (Capture), ELISA (Standard), ELISA Capture, Enzyme Assay Substrate, In Vivo, Library Screening, Western Blot.

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Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
All Species
Showing Publications 1 - 10 of 43. Show All 43 Publications.
Publications using 292-G2 Applications Species
CA Conover, LK Bale Senescence induces proteolytically-active PAPP-A secretion and association with extracellular vesicles in human pre-adipocytes Experimental gerontology, 2022-12-20;172(0):112070. 2022-12-20 [PMID: 36549546] (Bioassay, Human) Bioassay Human
JS Lee, MW Lero, J Mercado-Ma, S Zhu, M Jo, CE Tocheny, JS Morgan, LM Shaw The insulin and IGF signaling pathway sustains breast cancer stem cells by IRS2/PI3K-mediated regulation of MYC Cell Reports, 2022-12-06;41(10):111759. 2022-12-06 [PMID: 36476848] (Bioassay, Human) Bioassay Human
S Funakoshi, I Fernandes, O Mastikhina, D Wilkinson, T Tran, W Dhahri, A Mazine, D Yang, B Burnett, J Lee, S Protze, GD Bader, SS Nunes, M Laflamme, G Keller Generation of mature compact ventricular cardiomyocytes from human pluripotent stem cells Nature Communications, 2021-05-26;12(1):3155. 2021-05-26 [PMID: 34039977] (Bioassay, Human) Bioassay Human
CM Hammerle, I Sandovici, GV Brierley, NM Smith, WE Zimmer, I Zvetkova, HM Prosser, Y Sekita, BYH Lam, M Ma, WN Cooper, A Vidal-Puig, SE Ozanne, G Medina-Góm, M Constância Mesenchyme-derived IGF2 is a major paracrine regulator of pancreatic growth and function PLoS Genet, 2020-10-15;16(10):e1009069. 2020-10-15 [PMID: 33057429] (Western Blot, Mouse) Western Blot Mouse
L Mercurio, D Lulli, F Mascia, E Dellambra, C Scarponi, M Morelli, C Valente, ML Carbone, S Pallotta, G Girolomoni, C Albanesi, S Pastore, S Madonna Intracellular Insulin-like growth factor binding protein 2 (IGFBP2) contributes to the senescence of keratinocytes in psoriasis by stabilizing cytoplasmic p21 Aging (Albany NY), 2020-04-17;12(8):6823-6851. 2020-04-17 [PMID: 32302288] (Cell Culture, Human) Cell Culture Human
R Wang, X Qi, P Schmiege, E Coutavas, X Li Marked structural rearrangement of mannose 6-phosphate/IGF2 receptor at different pH environments Sci Adv, 2020-02-12;6(7):eaaz1466. 2020-02-12 [PMID: 32095534] (Binding Assay, Human) Binding Assay Human
I Rooda, K Hensen, B Kaselt, S Kasvandik, M Pook, A Kurg, A Salumets, A Velthut-Me Target prediction and validation of microRNAs expressed from FSHR and aromatase genes in human ovarian granulosa cells Sci Rep, 2020-02-10;10(1):2300. 2020-02-10 [PMID: 32042028] (Bioassay, Human) Bioassay Human
SM Garrett, E Hsu, JM Thomas, JM Pilewski, C Feghali-Bo Insulin-like growth factor (IGF)-II- mediated fibrosis in pathogenic lung conditions PLoS ONE, 2019-11-25;14(11):e0225422. 2019-11-25 [PMID: 31765403] (Bioassay, Human) Bioassay Human
C Fredolini, S Byström, L Sanchez-Ri, M Ioannou, D Tamburro, F Pontén, RM Branca, P Nilsson, J Lehtiö, JM Schwenk Systematic assessment of antibody selectivity in plasma based on a resource of enrichment profiles Sci Rep, 2019-06-06;9(1):8324. 2019-06-06 [PMID: 31171813] (ELISA Capture, Human) ELISA Capture Human
CA Conover, LK Bale, RL Frye, HV Schaff Cellular characterization of human epicardial adipose tissue: highly expressed PAPP-A regulates insulin-like growth factor I signaling in human cardiomyocytes Physiol Rep, 2019-02-01;7(4):e14006. 2019-02-01 [PMID: 30809969] (Bioassay, Human) Bioassay Human
Show All 43 Publications.

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Gene Symbol IGF2