Recombinant Human IGF-II/IGF2 Protein, CF


1 μg/lane of Recombinant Human IGF‑II/IGF2 was resolved with SDS-PAGE under reducing (R) conditions and visualized by silver staining, showing a single band at 7 kDa.
Recombinant Human IGF-II/IGF2 (Catalog # 292‑G2) stimulates cell proliferation in the MCF-7 human breast cancer cell line. The ED50 for this effect is 1.5‑6 ng/mL.

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Recombinant Human IGF-II/IGF2 Protein, CF Summary

Details of Functionality
Measured in a serum-free cell proliferation assay using MCF‑7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is 1.5‑6 ng/mL.
E. coli-derived human IGF-II/IGF2 protein
Accession #
N-terminal Sequence
Protein/Peptide Type
Recombinant Proteins
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Endotoxin Note
<0.10 EU per 1 μg of the protein by the LAL method.


Theoretical MW
7.5 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Read Publications using
292-G2 in the following applications:

Packaging, Storage & Formulations

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 3 months, -20 to -70 °C under sterile conditions after reconstitution.
Lyophilized from a 0.2 μm filtered solution in Acetic Acid.
>97%, by SDS-PAGE under reducing conditions and visualized by silver stain.
Reconstitution Instructions
Reconstitute at 100 μg/mL in sterile PBS.


This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for Recombinant Human IGF-II/IGF2 Protein, CF

  • C11orf43
  • chromosome 11 open reading frame 43
  • FLJ22066
  • FLJ44734
  • GRDF
  • IGF2
  • IGF-2
  • IGF-II
  • insulin-like growth factor 2 (somatomedin A)
  • insulin-like growth factor II
  • insulin-like growth factor type 2
  • MSA
  • PEG2
  • PP9974
  • somatomedin-A


Insulin-like growth factor I (also known as somatomedin C and somatomedin A) and insulin-like growth factor II (multiplication stimulating activity or MSA) belong to the family of insulin-like growth factors that are structurally homologous to proinsulin. Mature IGF-I and IGF-II share approximately 70% sequence identity. Both IGF-I and IGF-II are expressed in many tissues and cell types and may have autocrine, paracrine and endocrine functions. Mature IGF-I and IGF-II are highly conserved (100% identity between human, bovine and porcine proteins) and exhibit cross-species activity.

IGF-II is a potent mitogenic growth factor. However, unlike IGF-I which has important postnatal roles, the growth-promoting function of IGF-II is limited to embryonic development.

Two specific cell surface receptors that bind IGF-I and IGF-II have been identified. The type I IGF receptor that participates in IGF signaling is structurally related to the insulin receptor. It is a disulfide-linked heterotetrameric transmembrane glycoprotein with an intracellular tyrosine kinase domain. Type I IGF receptor binds IGF-I with higher affinity than IGF-II. The type II IGF receptor which binds IGF-II with much higher affinity than IGF-I is also the cation-independent mannose 6-phosphate receptor. At the present time, it is not known if the type II IGF receptor participates in the IGF signaling pathway. An additional unknown receptor which mediates IGF‑II signaling has also been proposed. Circulating IGFs exist in complexes bound to IGF binding proteins. Currently, at least six high affinity binding proteins have been identified.

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Publications for IGF-II/IGF2 (292-G2)(36)

We have publications tested in 8 confirmed species: Human, Mouse, Rat, Bovine, Chicken, N/A, Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque), Primate - Macaca nemestrina (Southern Pig-tailed Macaque).

We have publications tested in 7 applications: Bioassay, Cell Culture, ELISA (Capture), ELISA (Standard), Enzyme Assay Substrate, In Vivo, Library Screening.

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Primate - Macaca fascicularis (Crab-eating Monkey or Cynomolgus Macaque)
Primate - Macaca nemestrina (Southern Pig-tailed Macaque)
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Showing Publications 1 - 10 of 36. Show All 36 Publications.
Publications using 292-G2 Applications Species
L Mercurio, D Lulli, F Mascia, E Dellambra, C Scarponi, M Morelli, C Valente, ML Carbone, S Pallotta, G Girolomoni, C Albanesi, S Pastore, S Madonna Intracellular Insulin-like growth factor binding protein 2 (IGFBP2) contributes to the senescence of keratinocytes in psoriasis by stabilizing cytoplasmic p21 Aging (Albany NY), 2020;12(8):6823-6851. 2020 [PMID: 32302288] (Cell Culture, Human) Cell Culture Human
SM Garrett, E Hsu, JM Thomas, JM Pilewski, C Feghali-Bo Insulin-like growth factor (IGF)-II- mediated fibrosis in pathogenic lung conditions PLoS ONE, 2019;14(11):e0225422. 2019 [PMID: 31765403] (Bioassay, Human) Bioassay Human
CA Conover, LK Bale, RL Frye, HV Schaff Cellular characterization of human epicardial adipose tissue: highly expressed PAPP-A regulates insulin-like growth factor I signaling in human cardiomyocytes Physiol Rep, 2019;7(4):e14006. 2019 [PMID: 30809969] (Bioassay, Human) Bioassay Human
C De Giovann, P Nanni, L Landuzzi, ML Ianzano, G Nicoletti, S Croci, A Palladini, PL Lollini Immune targeting of autocrine IGF2 hampers rhabdomyosarcoma growth and metastasis BMC Cancer, 2019;19(1):126. 2019 [PMID: 30732578] (ELISA (Capture), Human) ELISA (Capture) Human
Z Chen, J Liu, D Chu, Y Shan, G Ma, H Zhang, XD Zhang, P Wang, Q Chen, C Deng, W Chen, DS Dimitrov, Q Zhao A dual-specific IGF-I/II human engineered antibody domain inhibits IGF signaling in breast cancer cells Int. J. Biol. Sci., 2018;14(7):799-806. 2018 [PMID: 29910690] (Library Screening, Human) Library Screening Human
R Sambathkum, R Akkerman, S Dastidar, P Roelandt, M Kumar, M Bajaj, AR Mestre Ros, N Helsen, V Vanslembro, E Kalo, S Khurana, J Laureys, C Gysemans, MM Faas, P de Vos, CM Verfaillie Generation of hepatocyte- and endocrine pancreatic-like cells from human induced endodermal progenitor cells PLoS ONE, 2018;13(5):e0197046. 2018 [PMID: 29750821] (Bioassay, Human) Bioassay Human
R Jalili, J Horecka, JR Swartz, RW Davis, HHJ Persson Streamlined circular proximity ligation assay provides high stringency and compatibility with low-affinity antibodies Proc. Natl. Acad. Sci. U.S.A., 2018;0(0):. 2018 [PMID: 29339495] (Bioassay) Bioassay
SP Guan, ATL Lam, JP Newman, KLM Chua, CYL Kok, ST Chong, MLK Chua, PYP Lam Matrix metalloproteinase-1 facilitates MSC migration via cleavage of IGF-2/IGFBP2 complex FEBS Open Bio, 2018;8(1):15-26. 2018 [PMID: 29321953] (Bioassay, Human) Bioassay Human
Y Liu, C Yu, Y Wu, X Sun, Q Su, C You, H Xin CD44(+) fibroblasts increases breast cancer cell survival and drug resistance via IGF2BP3-CD44-IGF2 signalling J. Cell. Mol. Med., 2017;0(0):. 2017 [PMID: 28523716] (ELISA (Standard), Human) ELISA (Standard) Human
Amber N Stratman Mural-Endothelial cell-cell interactions stabilize the developing zebrafish dorsal aorta Development, 2016;0(0):. 2016 [PMID: 27913637] (Bioassay, Human) Bioassay Human
Show All 36 Publications.

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Gene Symbol IGF2