| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NB300-514SS | |||
| Azide and BSA Free | NBP2-80785 | |||
AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." alt="Human whole blood monocytes were stained with Goat Anti-Human IGF-II R/IGF2R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2447, filled histogram) or control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107)." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | BAF2447 | |||
| Carrier Free | FAB2447F | |||
| Carrier Free | AF2447-SP | |||
NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." alt="IGF-II R/IGF2R was detected in immersion fixed A172 human glioblastoma cell line using Mouse Anti-Human IGF-II R/IGF2R Monoclonal Antibody (Catalog # MAB2447) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips." class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB2447-SP | |||
DY990). Goat Anti-Human IGF-II R/IGF2R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2447) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" alt="Human IGF-II R was serially diluted and captured by Mouse Anti-Human IGF-II R/IGF2R Monoclonal Antibody (Catalog # MAB24471) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human IGF-II R/IGF2R Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2447) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_thumb" />
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| Carrier Free | MAB24471-SP | |||
| Formulation | Catalog # | Availability | Price | |
|---|---|---|---|---|
| BSA Free | NBP1-84598-25ul | |||
![Immunocytochemistry/Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-IGF-II R/IGF2R Antibody (2G11) NB300-514 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.](http://images.novusbio.com/fullsize/IGF-II-R-IGF2R-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NB300-514-img0014.jpg "Immunocytochemistry/Immunofluorescence: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - HeLa cells were fixed in 4% paraformaldehyde for 10 minutes and permeabilized in 0.05% Triton X-100 in PBS for 5 minutes. The cells were incubated with anti-IGF-II R/IGF2R Antibody (2G11) NB300-514 at 1 ug/ml overnight at 4C and detected with an anti-mouse Dylight 488 (Green) at a 1:1000 dilution for 60 minutes. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 100X objective and digitally deconvolved.")
![Immunohistochemistry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining of human testis using NB300-514 antibody at 1:100.](http://images.novusbio.com/fullsize/Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-2G11-Immunohistochemistry-NB300-514-img0011.jpg)
![Flow Cytometry: IGF-II R/IGF2R Antibody (2G11) [NB300-514] - An intracellular stain was performed on MCF7 cells with IGF-II R/IGF2R [2G11] Antibody NB300-514AF647 (blue) and a matched isotype control (orange). Cells were fixed with 4% PFA and then permeabilized with 0.1% saponin. Cells were incubated in an antibody dilution of 5 ug/mL for 30 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 647.](http://images.novusbio.com/fullsize/IGF-II-R-IGF2R-Antibody-2G11-Flow-Cytometry-NB300-514-img0013.jpg)
![Immunocytochemistry/Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with or an antibody recognizing Mannose 6-Phosphate Receptor at a dilution of 1:20 over night at 4C, washed with PBS and incubated with a DyLight-488 conjugated.](http://images.novusbio.com/fullsize/Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NB300-514-img0006.jpg)
![Immunocytochemistry/Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - HMVEC Cells](http://images.novusbio.com/fullsize/Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NB300-514-img0007.jpg)
![Immunocytochemistry/Immunofluorescence: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Staining in Hela Cells. Mannose 6-Phosphate Receptor staining (green), F-Actin staining with Phalloidin (red) and nuclei with DAPI (blue) is shown. Cells were grown on chamber slides and fixed with formaldehyde prior to staining. Cells were probed without (control) or with an antibody recognizing Mannose 6-Phosphate Receptor at a dilution of 1:20 over night at 4 C, washed with PBS and incubated with a DyLight-488 conjugated secondary antibody.](http://images.novusbio.com/fullsize/Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NB300-514-img0008.jpg)
![Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Mannose 6 Phosphate Receptor (Cation independent) antibody (2G11) was tested at (1:250) in HeLa cells with Dylight 488 (green). Nuclei and alpha-tubulin were counterstained with DAPI (blue) and DyLight-550 (red).](http://images.novusbio.com/fullsize/Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-2G11-Immunocytochemistry-Immunofluorescence-NB300-514-img0010.jpg)
![Flow Cytometry: Mannose 6 Phosphate Receptor (Cation independent) Antibody (2G11) [NB300-514] - Intracellular flow cytometric staining of 1 x 10^6 MCF-7 cells using Mannose 6 Phosphate Receptor antibody (dark blue). Isotype control shown in orange. An antibody concentration of 1 ug/1x10^6 cells was used.](http://images.novusbio.com/fullsize/Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-2G11-Flow-Cytometry-NB300-514-img0012.jpg)
NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for 
NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm. View our protocol for 
CTS008) and counterstained with hematoxylin (blue). View our protocol for 
MAB24471) coated on a Clear Polystyrene Microplate (Catalog # ![Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR was performed by loading various amounts of HeLa and Jurkat whole cell lysates per lane.](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-Western-Blot-NB100-74387-img0012.jpg "Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR was performed by loading various amounts of HeLa and Jurkat whole cell lysates per lane.")
![Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm and nucleus of MCF-7 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74387-img0011.jpg)
![Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm of NIH-3T3 cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74387-img0009.jpg)
![Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NB100-74387] - Analysis of CI-MPR (green) showing staining in the cytoplasm of Hela cells (right) compared to a negative control without primary antibody (left).](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-Immunocytochemistry-Immunofluorescence-NB100-74387-img0010.jpg)
![Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Fig. 1. Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line. Lane 1: immunostaining with anti-CD222 (MEM-238) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-MEM-238-Western-Blot-NB500-464-img0001.jpg "Western Blot: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Fig. 1. Western Blotting analysis (non-reducing conditions) of CD222 in whole cell lysate of JURKAT human peripheral blood T cell leukemia cell line. Lane 1: immunostaining with anti-CD222 (MEM-238) Lane 2: immunostaining with Isotype mouse IgG1 control (PPV-06).")
![Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [FITC] [NB100-63599] - Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238)[NB100-63599]- Staining human peripheral blood monocytes following permeablisation with Leucoperm (Fix & Perm) (BUF09). Image using the FITC form of this antibody.](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-MEM-238-Flow-Cytometry-NB500-464-img0004.jpg)
![Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Intracellular staining of human peripheral blood with anti-CD222 (MEM-238) PE.](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-MEM-238-Flow-Cytometry-NB500-464-img0002.jpg)
![Flow Cytometry: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody (MEM-238) [NB500-464] - Surface staining of human peripheral blood with anti-CD222 (MEM-238) biotin, streptavidin-APC](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-MEM-238-Flow-Cytometry-NB500-464-img0003.jpg)
![N/A IGF-II R/IGF2R [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_IGFII_R_DY2447_ELISA_1831.jpg "N/A IGF-II R/IGF2R [Biotin]")
![Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NBP1-84598] - Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-84598-img0004.jpg "Immunocytochemistry/Immunofluorescence: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NBP1-84598] - Immunofluorescent staining of human cell line U-251 MG shows localization to the Golgi apparatus.")
![Immunohistochemistry-Paraffin: IGF-II R/Mannose 6 Phosphate Receptor (Cation independent) Antibody [NBP1-84598] - Staining of human placenta shows strong cytoplasmic positivity in trophoblastic cells.](http://images.novusbio.com/fullsize/IGF-II-R-Mannose-6-Phosphate-Receptor-Cation-independent-Antibody-Immunohistochemistry-Paraffin-NBP1-84598-img0005.jpg)
![Western Blot: IGF-II R/IGF2R Antibody (4J4A3) [NBP3-16270] - Western blot analysis of extracts of A-549 cells, using Cation-independent IGF-II R/IGF2R (IGF2R) Rabbit mAb (NBP3-16270) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](http://images.novusbio.com/fullsize/IGF-II-R-IGF2R-Antibody-4J4A3-Western-Blot-NBP3-16270-img0003.jpg "Western Blot: IGF-II R/IGF2R Antibody (4J4A3) [NBP3-16270] - Western blot analysis of extracts of A-549 cells, using Cation-independent IGF-II R/IGF2R (IGF2R) Rabbit mAb (NBP3-16270) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunocytochemistry/Immunofluorescence: IGF-II R/IGF2R Antibody (4J4A3) [NBP3-16270] - NIH/3T3 cells using Cation-independent M6PR (IGF2R) Rabbit mAb (dilution 1:100)(Red). The cells were counterstained with alpha -Tubulin Mouse mAb (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.](http://images.novusbio.com/fullsize/nbp3-16270_rabbit-igf-ii-r-igf2r-mab-4j4a3-immunocytochemistry-immunofluorescence-6102023112511..jpg)
![Western Blot: IGF-II R/IGF2R Antibody (4J4A3) [NBP3-16270] - Analysis of extracts of Mouse heart, using Cation-independent Cation-independent M6PR (IGF2R) (IGF2R) Rabbit mAb at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit. Exposure time: 3min.](http://images.novusbio.com/fullsize/nbp3-16270_rabbit-igf-ii-r-igf2r-mab-4j4a3-western-blot-6102023112732..jpg)
![Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Detection of CD222 in human peripheral blood with anti-CD222 (MEM-240) purified, GAM-APC.](http://images.novusbio.com/fullsize/IGF-II-R-IGF2R-Antibody-MEM-240-Flow-Cytometry-NBP3-09145-img0003.jpg "Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Detection of CD222 in human peripheral blood with anti-CD222 (MEM-240) purified, GAM-APC.")
![Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/IGF-II-R-IGF2R-Antibody-MEM-240-Flow-Cytometry-NBP3-09145-img0001.jpg)
![Flow Cytometry: IGF-II R/IGF2R Antibody (MEM-240) [NBP3-09145] - Separation of human neutrophil granulocytes (red-filled) from lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD222 (MEM-240) purified antibody (concentration in sample 3 ug/ml) GAM APC.](http://images.novusbio.com/fullsize/IGF-II-R-IGF2R-Antibody-MEM-240-Flow-Cytometry-NBP3-09145-img0002.jpg)
