ICAM-1/CD54 Antibody [Biotin]

ICAM-1/CD54 was detected in immersion fixed paraffin-embedded sections of human kidney using Goat Anti-Mouse ICAM-1/CD54 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF796) at 5 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to cell membrane in convoluted tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

• Reactivity: Mu
• Applications: WB, IHC, ELISA(Det)
• Clonality: Polyclonal
• Host: Goat
• Conjugate: Biotin
• Concentration: LYOPH

ICAM-1/CD54 Antibody [Biotin] Summary

• Immunogen: Mouse myeloma cell line NS0-derived recombinant mouse ICAM‑1/CD54
  Gln28-Asn485
  Accession # Q3U8M7
• Specificity: Detects mouse ICAM-1/CD54 in ELISAs and Western blots. In sandwich ELISAs, less than 0.3% cross-reactivity with recombinant human ICAM-1, recombinant rat ICAM-1, and recombinant mouse ICAM-2 is observed.
• Source: N/A
• Isotype: IgG
• Clonality: Polyclonal
• Host: Goat
• Gene: ICAM1
• Purity Statement: Antigen Affinity-purified

Applications/Dilutions

• Dilutions:
  • ELISA Capture (Matched Antibody Pair) 2-8 ug/mL
  • ELISA Detection (Matched Antibody Pair) 0.1-0.4 ug/mL
  • ELISA Standard (Matched Pair)
  • Immunohistochemistry 2-10 ug/mL
  • Western Blot 0.1 ug/mL
• Application Notes: ELISA Capture: Mouse ICAM-1/CD54 Antibody (Catalog # MAB796) ELISA Detection: Mouse ICAM-1/CD54 Biotinylated Antibody (Catalog # BAF796) Standard: Recombinant Mouse ICAM-1/CD54 Fc Chimera (Catalog # 796-IC)
• Readout System:
  • Streptavidin Native Protein NBP2-29367
  • Streptavidin Native Protein NBP2-29370
  • Streptavidin Native Protein NBP2-29371

Packaging, Storage & Formulations

• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 degreesC as supplied. 1 month, 2 to 8 degreesC under sterile conditions after reconstitution. 6 months, -20 to -70 degreesC under sterile conditions after reconstitution.
• Buffer: Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
• Preservative: No Preservative
• Concentration: LYOPH
• Reconstitution Instructions: Reconstitute at 0.2 mg/mL in sterile PBS.

Notes

This product is produced by and ships from R&D Systems, Inc., a Bio-Techne brand.

Alternate Names for ICAM-1/CD54 Antibody [Biotin]

• BB2
• CD54 antigen
• CD54
• cell surface glycoprotein P3.58
• human rhinovirus receptor
• ICAM1
• ICAM-1
• intercellular adhesion molecule 1 (CD54), human rhinovirus receptor
• intercellular adhesion molecule 1
• Major group rhinovirus receptor
• P3.58

Background

Intercellular Adhesion Molecule-1 (ICAM-1, CD54) binds the leukocyte integrins LFA-1 and Mac-1. ICAM-1 expression is weak on leukocytes, epithelial and resting endothelial cells, as well as some other cell types, but expression can be stimulated by IFN-gamma , TNF-alpha , IL-1 beta and LPS. Mouse and human ICAM-1 share approximately 54% amino acid identity. Soluble ICAM-1 is found in a biologically active form in serum, probably as a result of proteolytic cleavage from the cell surface, and is elevated in patients with various inflammatory syndromes such as septic shock, leukocyte adhesion deficiency syndrome (LAD), cancer and transplantation.

1. Pigott, R. and C. Power (1993) in The Adhesion Molecule Facts Book. Academic Press, p. 74.
2. Siu, G. et al. (1989) J. Immunol. 143:3813.
3. Ballantyne, C.M. et al. (1989) Nuc. Acid. Res. 17:5853.

Limitations

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Publications for ICAM-1/CD54 Antibody (BAF796)(3)

Publications using BAF796

• Mastrogiacomo L, Werstuck GH Investigating the Role of Endothelial Glycogen Synthase Kinase3?/? in Atherogenesis in Low Density Lipoprotein Receptor Knockout Mice International journal of molecular sciences 2022-11-26 [PMID: 36499109]
  
  Details:
  Dilutions: 1:50
• Trey E. Gilpin, Fruzsina R. Walter, Melinda Herbath, Matyas Sandor, Zsuzsanna Fabry Mycobacterium bovis Bacillus Calmette–Guérin–Infected Dendritic Cells Induce TNF-alpha –Dependent Cell Cluster Formation That Promotes Bacterial Dissemination through an In Vitro Model of the Blood–Brain Barrier The Journal of Immunology 2021-08-15 [PMID: 34321229]
• Zhang M, Yin L, Zhang K, Sun W, Yang S, Zhang B, Salzman P, Wang W, Liu C, Vidyasagar S, Zhang L, Ju S, Okunieff P, Zhang L Response patterns of cytokines/chemokines in two murine strains after irradiation. Cytokine, 2012-01-25;58(2):169-77. 2012-01-25 [PMID: 22277799] (Luminex Development, Mouse)

Reviews for ICAM-1/CD54 Antibody (BAF796) (3)

Average Rating: 4.3

(Based on 3 reviews)

We have 3 reviews tested in 1 species: Mouse.

reviewed by: Verified Customer IF Mouse 01/15/2015

Summary

• Application: Immunofluorescence
• Sample Tested: See PMID: 22198574
• Species: Mouse

reviewed by: Verified Customer IHC-P Mouse 01/15/2015

Summary

• Application: Immunohistochemistry-Paraffin
• Sample Tested: See PMID: 22687510
• Species: Mouse

reviewed by: Verified Customer IHC-Fr Mouse 01/15/2015

Summary

• Application: Immunohistochemistry-Frozen
• Sample Tested: See PMID: 22198574
• Species: Mouse

Bioinformatics

• Gene Symbol: ICAM1
• Uniprot:
  • Q3U8M7()