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CTS008) and counterstained with hematoxylin (blue). Specific staining was localized to plasma membrane in Leydig cells. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections." class="big_lightbox" target="_blank">
796-IC) was serially diluted and captured by Rat Anti-Mouse ICAM‑1/CD54 Monoclonal Antibody (Catalog # MAB796) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Mouse ICAM‑1/CD54 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF796) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)" class="big_lightbox" target="_blank">
HAF018). A specific band was detected for ICAM‑1/CD54 at approximately 85 kDa (as indicated). This experiment was conducted under non-reducing conditions and using Western Blot Buffer Group 1." class="big_lightbox" target="_blank">
NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surface and cytoplasm." class="big_lightbox" target="_blank">
MAB002) followed by anti-Mouse IgG PE-conjugated Secondary Antibody (F0102B) and Mouse Anti-Human CD14 APC-conjugated Monoclonal Antibody (FAB3832A). Staining was performed using our Staining Membrane-associated Proteins protocol." class="big_lightbox" target="_blank">
ICAM1 is a 85-110 kDa single chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. It shares considerable amino acid sequence homology with ICAM3 and with ICAM2. ICAM1 is expressed by activated endothelial cells. It is detected on cells of many other lineages (e.g. epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils), with lower levels that increase in inflammation. ICAM1 is also detected in some carcinoma and melanoma cells. Soluble ICAM1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes. It is the receptor for rhinoviruses and malaria.
![Immunohistochemistry ICAM-1/CD54 Antibody [Unconjugated]](http://images.novusbio.com/images/antibody/ICAM1_AF796_Immunohistochemistry_17354.jpg)
![Western Blot ICAM-1/CD54 Antibody (BBIG-I1) [Unconjugated]](http://images.novusbio.com/images/antibody/bba3_human-icam-1-cd54-mab-clone-bbig-i1-western-blot-194202263529.jpg)
![Sandwich ELISA ICAM-1/CD54 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79672-img0001.jpg)
![Sandwich ELISA ICAM-1/CD54 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79620-img0001.jpg)
![Sandwich ELISA ICAM-1/CD54 Antibody Pair [HRP]](http://images.novusbio.com/fullsize/ICAM-1-CD54-Antibody-Pair-[HRP]-Sandwich-ELISA-NBP2-79586-img0001.jpg)
![N/A ICAM-1/CD54 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_ICAM1_DCD540_ELISA_622.jpg)
![N/A ICAM-1/CD54 [Biotin]](http://images.novusbio.com/fullsize/elisa/DATA_ICAM1_DY720_ELISA_2193.jpg)
![N/A ICAM-1/CD54 [HRP]](http://images.novusbio.com/fullsize/elisa/DATA_ICAM1_MIC100_ELISA_986.jpg)
![SDS-Page ICAM-1/CD54 [Unconjugated]](http://images.novusbio.com/fullsize/protein/ICAM-1_720-IC_764.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-4120241048112.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-41202410481112.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-4120241049436.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-41202410481192.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-812025555325.jpg)
![Western Blot ICAM-1/CD54 Antibody [Unconjugated]](http://images.novusbio.com/fullsize/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-western-blot-12122025925915.jpg)
![ELISA ICAM-1/CD54 Antibody [Unconjugated]](http://images.novusbio.com/images/af796_mouse-icam-1-cd54-affinity-purified-polyclonal-ab-elisa-639201852915851164.jpg)
![Immunocytochemistry ICAM-1/CD54 Antibody (BBIG-I1) [Unconjugated]](http://images.novusbio.com/images/antibody/bba3_human-icam-1-cd54-mab-clone-bbig-i1-immunocytochemistry-1242022102751.jpg)
![Flow Cytometry ICAM-1/CD54 Antibody (BBIG-I1) [Unconjugated]](http://images.novusbio.com/images/antibody/bba3_human-icam-1-cd54-mab-clone-bbig-i1-flow-cytometry-184202217820.jpg)
![Immunohistochemistry ICAM-1/CD54 Antibody (BBIG-I1) [Unconjugated]](http://images.novusbio.com/fullsize/bba3_human-icam-1-cd54-mab-clone-bbig-i1-immunohistochemistry-12122025748134.jpg)
![Immunohistochemistry ICAM-1/CD54 Antibody (BBIG-I1) [Unconjugated]](http://images.novusbio.com/fullsize/bba3_human-icam-1-cd54-mab-clone-bbig-i1-immunohistochemistry-121220257453112.jpg)
![Immunocytochemistry/ Immunofluorescence ICAM-1/CD54 Antibody (BBIG-I1) [Unconjugated]](http://images.novusbio.com/fullsize/bba3_human-icam-1-cd54-mab-clone-bbig-i1-immunocytochemistry-immunofluorescence-121220255555514.jpg)
![Western Blot ICAM-1/CD54 Antibody (BBIG-I1) [Unconjugated]](http://images.novusbio.com/fullsize/bba3_human-icam-1-cd54-mab-clone-bbig-i1-western-blot-12122025817120.jpg)
![Western Blot ICAM-1/CD54 Antibody (BBIG-I1) [Unconjugated]](http://images.novusbio.com/fullsize/bba3_human-icam-1-cd54-mab-clone-bbig-i1-western-blot-121220258222415.jpg)
![N/A ICAM-1/CD54 [HRP]](http://images.novusbio.com/fullsize/elisa/ICAM-1_DCD540_ELISA_58.jpg)
![N/A ICAM-1/CD54 [HRP]](http://images.novusbio.com/fullsize/elisa/ICAM-1_MIC100_ELISA_279.jpg)
